A5054040170- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- RNA Interference and Gene Delivery
- Cell death mechanisms and regulation
- Bacterial Genetics and Biotechnology
- Endoplasmic Reticulum Stress and Disease
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- DNA and Nucleic Acid Chemistry
- Metabolism and Genetic Disorders
- Signaling Pathways in Disease
- Cellular transport and secretion
- Sphingolipid Metabolism and Signaling
- Peptidase Inhibition and Analysis
- Photoreceptor and optogenetics research
- Conducting polymers and applications
- Photochromic and Fluorescence Chemistry
- Immunodeficiency and Autoimmune Disorders
- Electrochemical Analysis and Applications
- Porphyrin and Phthalocyanine Chemistry
- Fuel Cells and Related Materials
- Antibiotics Pharmacokinetics and Efficacy
University of Tübingen
2014-2022
University of the Basque Country
2010-2020
Fundación Biofísica Bizkaia
2018-2020
Instituto Biofisika
2017-2018
Max Planck Institute for Intelligent Systems
2014-2016
Max Planck Society
2013
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2010
Institute for Research in Biomedicine
2010
Instituto de Investigaciones Químicas
2010
Institute of Advanced Chemistry of Catalonia
2010
Article13 January 2022Open Access Source DataTransparent process DRP1 interacts directly with BAX to induce its activation and apoptosis Andreas Jenner orcid.org/0000-0002-6388-3053 Institute for Genetics, CECAD, University of Cologne, Germany Interfaculty Biochemistry, Tübingen, Contribution: Formal analysis (equal), Investigation Visualization Methodology Writing - review & editing (equal) Search more papers by this author Aida Peña-Blanco Raquel Salvador-Gallego...
Drp1 is a dynamin-like GTPase that mediates mitochondrial and peroxisomal division in process dependent on self-assembly coupled to GTP hydrolysis. Despite the link between malfunction human disease, molecular details of its membrane activity remain poorly understood. Here we reconstituted directly visualized giant unilamellar vesicles. We quantified effect lipid composition binding remodeling by fluorescence confocal microscopy flow cytometry. In contrast other dynamin relatives, bound both...
Abstract The proteins of the Bcl-2 family have a crucial role in mitochondrial outer membrane permeabilization during apoptosis and regulation dynamics. Current models consider that Bax forms toroidal pores at mitochondria are responsible for release cytochrome c, whereas Bcl-xL inhibits pore formation. However, how regulate fission fusion remains poorly understood. By using systematic analysis single vesicle level, we found cBid, able to remodel membranes different ways. cBid induced...
Oligonucleotide conjugates carrying carbohydrates at the 5′-end have been prepared. Glucose, fucose, and saccharides containing glucose nonreducing end were attached to DNA strands using classical phosphoramidite chemistry. Two types of spacers a dendron scaffold helped obtain diversity sugar presentations in conjugates. Cellular surface adsorption cellular uptake carbohydrate oligonucleotide antisense sequences measured flow cytometric analysis. Conjugates with moiety linked through long...
Dynamin-related protein 1 (Drp1), an 80 kDa mechanochemical GTPase of the dynamin superfamily, is required for mitochondrial division in mammals. Despite role Drp1 dysfunction human disease, its molecular mechanism remains poorly understood. Here, we examined effect on membrane curvature using tubes pulled from giant unilamellar vesicles (GUVs). We found that GTP promoted rapid rearrangement a uniform distribution to discrete foci, line with assembly scaffolds at multiple nucleation sites...
BFL1 is a relatively understudied member of the BCL2 protein family which has been implicated in pathogenesis and chemoresistance variety human cancers, including hematological malignancies solid tumours. generally considered to have an antiapoptotic function, although its precise mode action remains unclear. By quantitatively analyzing synthetic membrane models cells, we found that inhibits apoptosis through three distinct mechanisms are similar but not identical those BCLXL, paradigmatic...
Type IV Coupling Proteins (T4CPs) are essential elements in many type secretion systems (T4SSs). The members of this family display sequence, length, and domain architecture heterogeneity, being the conserved Nucleotide-Binding Domain motif that defines them. In addition, most T4CPs contain a Transmembrane (TMD) amino end an All-Alpha facing cytoplasm. Additionally, few present variable at carboxyl end. structural paradigm is TrwBR388, T4CP conjugative plasmid R388. This protein has been...
Photosensitive fluorophores, which emission can be controlled using light, are essential for advanced biological imaging, enabling precise spatiotemporal tracking of molecular features, and facilitating super-resolution microscopy techniques. While irreversibly photoactivatable fluorophores well established, reversible reporters re-activated multiple times remain scarce, only few have been applied in living cells generalizable protein labelling methods. To address these limitations, we...
Giza immunoeskasiaren birusa (GIBa) oldar sartzea nahiko berria izan arren, mundu mailako osasun-arazo publiko bihurtu da. WHO [1]-aren arabera, 2016ra arte heriotza-arrazoietan lehenengo hamarretako bat zen, eta nahiz haren agerpenak herrialde garatuen osasun-sistemetan erreakzio motela eragin, laster, gehien ikerturiko birusetakoa bilakatu zen. Gaur egun, biologiaren, bizi-zikloaren eragindako gaixotasuna den hartutako sindromeari (HIESari) buruzko fisiopatologiaren inguruko xehetasun...