A5063584593- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Rabies epidemiology and control
- Electrocatalysts for Energy Conversion
- Mosquito-borne diseases and control
- Plant Virus Research Studies
University of Toronto
2019-2024
Hospital for Sick Children
2024
National Institute of Technology Hamirpur
2020
University of Saskatchewan
2018-2020
CRISPR-Cas9 systems provide powerful tools for genome editing. However, optimal employment of this technology will require control Cas9 activity so that the timing, tissue specificity, and accuracy editing may be precisely modulated. Anti-CRISPR proteins, which are small, naturally occurring inhibitors CRISPR-Cas systems, well suited purpose. A number anti-CRISPR proteins have been shown to potently inhibit subgroups but their maximal inhibitory is generally restricted specific homologs....
Abstract Phages and other mobile genetic elements express anti-CRISPR proteins (Acrs) to protect their genomes from destruction by CRISPR–Cas systems. Acrs usually block the ability of systems bind or cleave nucleic acid substrates. Here, we investigate an unusual Acr, AcrIF9, that induces a gain-of-function type I-F (Csy) complex, causing it strongly DNA lacks both PAM sequence complementarity. We show specific non-specific dsDNA compete for same site on Csy:AcrIF9 complex with rapid...
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) proteins serve as a formidable defense mechanism for bacteria against foreign DNA; on the other hand, some bacteriophages (phages) mobile genetic elements have evolved anti-CRISPR (Acr) to counteract CRISPR-Cas systems ensure their own survival. Because Acr provide phages with fitness advantage relative that they infect, accurately identifying inhibit has potential significantly positively impact...
Total genomic (g)DNA from 100 American dog ticks (Dermacentor variabilis) collected humans, dogs, raccoons, and skunks near Minnedosa (Manitoba, Canada) in 2005 was tested for the presence of Moellerella wisconsensis (Gammaproteobacteria: Enterobacteriales) using PCR. Although two gDNA samples derived attached to striped (Mephitis mephitis) contained M. DNA, it is unlikely that D. variabilis a vector this bacterium. Genomic DNA prepared washes external surfaces these (i.e., before extraction...
CRISPR-Cas9 systems provide powerful tools for genome editing. However, optimal employment of this technology will require control Cas9 activity so that the timing, tissue specificity, and accuracy editing may be precisely modulated. Anti-CRISPR proteins, which are small naturally-occurring inhibitors CRISPR-Cas systems, well-suited purpose. A number anti-CRISPR proteins have been shown to potently inhibit subgroups but their maximal inhibitory is generally restricted specific homologs....
ABSTRACT Phages and other mobile genetic elements express anti-CRISPR proteins (Acrs) to protect their genomes from destruction by CRISPR-Cas systems. Acrs usually block the ability of systems bind or cleave nucleic acid substrates. Here, we investigate an unusual Acr, AcrIF9, that induces a gain-of-function type I-F (Csy) complex, causing it strongly DNA lacks both PAM sequence complementarity. We show specific non-specific dsDNA compete for same site on Csy:AcrIF9 complex with rapid...