A5033251507- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Nanopore and Nanochannel Transport Studies
- Advanced biosensing and bioanalysis techniques
- Advanced Fluorescence Microscopy Techniques
- Immunotherapy and Immune Responses
- Advanced Electron Microscopy Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Electrostatics and Colloid Interactions
- Advanced Physical and Chemical Molecular Interactions
- DNA and Nucleic Acid Chemistry
- Force Microscopy Techniques and Applications
Canada's Michael Smith Genome Sciences Centre
2021-2025
University of British Columbia
2021-2025
McGill University
2016-2021
Nanoparticles are a promising solution for delivery of wide range medicines and vaccines. Optimizing their design depends on being able to resolve, understand, predict biophysical therapeutic properties, as function parameters. While existing tools have made great progress, gaps in understanding remain because the inability make detailed measurements multiple correlated properties. Typically, an average measurement is across heterogeneous population, obscuring potentially important...
Lipid nanoparticles (LNPs) have proven to be promising delivery vehicles for RNA-based vaccines and therapeutics, particularly in LNP formulations containing ionizable cationic lipids that undergo protonation/deprotonation response buffer pH changes. These are typically formulated using a rapid mixing technique at low pH, followed by return physiological triggers LNP-LNP fusion. A detailed understanding of these dynamic processes is crucial optimize the overall performance efficiency LNPs....
Lipid nanoparticles (LNPs) are widely used for delivering therapeutic nucleic acids, yet the relationship between their internal structure and intracellular behavior, particularly before RNA release, remains unclear. Here, we elucidate how lipid-siRNA organization within LNPs can modulate delivery dynamics. We use cryo-electron microscopy photochemical assays to reveal that increased siRNA loading reduce helper lipids' distribution LNP surface, while consistently localizes near surface....
We directly measure the free energy of confinement for semiflexible polymers from nanoscale to bulk regimes in slit-like confinement. use convex lens-induced (CLiC) microscopy DNA count molecules at equilibrium a single chamber smoothly increasing height. Our data, acquired across continuum regimes, provide bridge with which connect scaling theories established qualitatively different regimes. present new experimental data and simulations that Odijk theory describing sub-persistence-length...