A5100447009- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Immunotherapy and Immune Responses
- DNA and Nucleic Acid Chemistry
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Nanoplatforms for cancer theranostics
- Spectroscopy and Quantum Chemical Studies
- Orthopaedic implants and arthroplasty
- Traditional and Medicinal Uses of Annonaceae
- Protein Structure and Dynamics
- RNA Research and Splicing
- Metabolism, Diabetes, and Cancer
- Nuclear Receptors and Signaling
- Microbial Community Ecology and Physiology
- TGF-β signaling in diseases
- Heat shock proteins research
- Neonatal Health and Biochemistry
- Genomics and Phylogenetic Studies
- Phytochemistry and Bioactive Compounds
- Nanopore and Nanochannel Transport Studies
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- Signaling Pathways in Disease
Tianjin Medical University
2024
University of British Columbia
2022-2024
Canada's Michael Smith Genome Sciences Centre
2024
Stomatological Hospital of Chongqing Medical University
2022
Chongqing Medical University
2022
Guangzhou Medical University
2021
Jiangsu Province Hospital
2021
Nanjing Medical University
2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
Guangzhou First People's Hospital
2021
The transfection potency of lipid nanoparticle (LNP) mRNA systems is critically dependent on the ionizable cationic component. LNP composed optimized lipids often display distinctive mRNA-rich "bleb" structures. Here, it shown that such structures can also be induced for LNPs containing nominally less active by formulating them in presence high concentrations pH 4 buffers as sodium citrate, leading to improved potencies both vitro and vivo. Induction bleb structure type buffer employed, with...
Lipid nanoparticle (LNP) formulations are a proven method for the delivery of nucleic acids gene therapy as exemplified by worldwide rollout LNP-based RNAi therapeutics and mRNA vaccines. However, targeting specific tissues or cells is still major challenge. After LNP administration, LNPs interact with biological fluids (i.e., blood), components which adsorb onto surface forming layer biomolecules termed “biomolecular corona (BMC)” affects stability, biodistribution, tissue tropism. The...
Abstract Clinical deployment of oligonucleotides requires delivery technologies that improve stability, target tissue accumulation and cellular internalization. Exosomes show potential as ideal vehicles. However, an affordable generalizable system for efficient loading on exosomes remain lacking. Here, we identified Exosomal Anchor DNA Aptamer (EAA) via SELEX against immobilized with our proprietary CP05 peptides. EAA shows high binding affinity to different enables nucleic acid drugs...
Lipid nanoparticles (LNPs) have proven to be promising delivery vehicles for RNA-based vaccines and therapeutics, particularly in LNP formulations containing ionizable cationic lipids that undergo protonation/deprotonation response buffer pH changes. These are typically formulated using a rapid mixing technique at low pH, followed by return physiological triggers LNP-LNP fusion. A detailed understanding of these dynamic processes is crucial optimize the overall performance efficiency LNPs....
There are many opportunities to use macromolecules, such as peptides and oligonucleotides, for intracellular applications. Despite this, general methods delivering these molecules the cytosol in a safe efficient manner not available. Efforts develop variety of drug delivery systems viral vectors, lipoplexes, nanoparticles, amphiphilic have been made, but various challenges efficiency, toxicity, controllability remain. A central challenge is ability selectively perturb, destroy, membrane...
Nucleic acid therapeutics represent a major advance toward treating diseases at their root cause. However, nucleic acids are prone to degradation by serum endonucleases, clearance through the immune system, and rapid in complex medium. To overcome these barriers, frequently include chemical modifications improve stability or decrease responses. Lipid nanoparticles (LNPs) have enabled dramatic reduction dose required achieve therapeutic effect protecting improving intracellular delivery. It...
Abstract Phytochemical investigation on the stem bark of Aphanamixis grandifolia afforded five novel tirucallane‐type triterpenoids, (13 α ,14 β ,17 ,23 Z )‐25‐methoxy‐21,23‐epoxylanosta‐7,20(22),23‐triene‐3,21‐dione ( 1 ), )‐21,23‐epoxylanosta‐7,20(22),23,25‐tetraene‐3,21‐dione 2 (3 R ,5 , 9 ,10 ,13 S )‐17‐{(2 ,3 )‐5‐[(2 )‐3,3‐dimethyloxiran‐2‐yl]‐2,3,4,5‐tetrahydro‐2,5‐dimethoxyfuran‐3‐yl}‐4,4,10,13,14‐pentamethyl‐2,3,4,5,6,9,10,11,12,13,14,15,16,17‐tetradecahydro‐1 H ‐cyclopenta[ a...
Ribavirin and lamivudine are representatives of antiviral drugs that widely used to treat viral infections, especially chronic liver disease. To compare binding mechanism behavior with human serum albumin ( HSA ), we performed fluorescence spectroscopy X‐ray crystallography investigate the interactions ribavirin . Fluorescence showed inhibit affinity each other. Our results further demonstrated lamivdudine interaction could be affected by presence other compounds, including non‐steroidal...
The taxonomic position of a novel actinomycete, strain TRM 46250T, isolated from the sediment salt water beach at Baicheng, Xinjiang, China, was determined by polyphasic approach. Strain 46250T grew optimally in presence 2 % (w/v) NaCl and an optimum temperature range for growth 28-37 °C. whole-cell sugars were ribose, xylose, mannose galactose. diagnostic diamino acid meso-diaminopimelic acid. polar lipids phosphatidylinositol, phosphatidylcholine, phosphatidylethanolamine,...
Feline sarcoma-related protein (FER) is known to play a critical regulatory role in several carcinomas. However, the exact biological function of FER hepatocellular carcinoma (HCC) still needs be investigated. The primary objective this research was investigate unknown and molecular mechanisms HCC.The expression level HCC tissue samples cells examined by RT-qPCR, immunohistochemistry western blot. Cellular animal experiments were used explore effect on proliferative metastatic capacities...
Periodontal ligament mesenchymal stem cells (PDLSCs) are a promising cell resource for cell-based regenerative medicine in dentistry, but they inevitably acquire senescent phenotype after prolonged vitro expansion. The key regulators of PDLSCs during replicative senescence remain unclear. Here, we sought to elucidate the role metabolomic changes determining cellular PDLSCs. were cultured passages 4, 10 and 20. phenotypes detected, metabolomics analysis was performed. We found that manifested...
OBJECTIVES To study the effect of puromycin aminonucleoside (PAN) on apoptosis mouse podocyte clone 5 (MPC-5) and expression recombinant human Parkinson's disease 7 (Park7) to protective mechanism tacrolimus (FK506) against MPC-5 injury. METHODS cells were cultured in vitro then divided into three groups: blank control (control), PAN, FK506. The PAN group added with (with a concentration 50 mg/L) establish model injury, those FK506 mg/L). An inverted microscope was used observe morphology...