A5019672015- Pharmacogenetics and Drug Metabolism
- Estrogen and related hormone effects
- Glycosylation and Glycoproteins Research
- Drug Transport and Resistance Mechanisms
- Muscle Physiology and Disorders
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Neonatal Health and Biochemistry
- Prostate Cancer Treatment and Research
- Genomics and Chromatin Dynamics
- Corneal Surgery and Treatments
- Biochemical and Molecular Research
- Ocular Surface and Contact Lens
- Inflammatory mediators and NSAID effects
- RNA Research and Splicing
- MicroRNA in disease regulation
- Cancer therapeutics and mechanisms
- Erythrocyte Function and Pathophysiology
- Proteoglycans and glycosaminoglycans research
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Diet, Metabolism, and Disease
- Ubiquitin and proteasome pathways
Flinders University
2016-2025
Flinders Medical Centre
2012-2022
Scripps Research Institute
1999-2010
Neurosciences Institute
2000-2009
Ashok Leyland (India)
2009
Ashoka
2009
John Jay College of Criminal Justice
2005
Children's Hospital of Philadelphia
2001
University of Pennsylvania
2001
University of Colorado Health
2001
Manipulation of the interaction a morphogen with extracellular matrix changes its biological activities by altering diffusion.
Aims Demonstrate the presence of cytochrome P450 (CYP) and UDP‐glucuronosyltransferase (UGT) proteins mRNAs in isolated human plasma exosomes evaluate capacity for exosome‐derived biomarkers to characterize variability CYP3A4 activity. Methods The CYP UGT protein mRNA from HepaRG cell culture medium was determined by mass spectrometry reverse transcription–polymerase chain reaction, respectively. concordance between midazolam apparent oral clearance (CL/F) evaluated a small proof‐of‐concept...
UDP-glucuronosyltransferases (UGTs) comprise an important enzyme system in mammals that is involved detoxification of a variety small hydrophobic compounds both endogenous and exogenous origin. Some evidence suggests these enzymes may function as oligomers; however, little known about the domain interaction or mechanism oligomerization. In this work, for functional dimerization between UGTs provided by studies on mutated forms UGT2B1. When two inactive UGT2B1 were co-expressed cell culture,...
The UDP glycosyltransferases (UGT) attach sugar residues to small lipophilic chemicals alter their biological properties and enhance elimination. Of the four families present in mammals, two families, UGT1 UGT2, use glucuronic acid glucuronidate bilirubin, steroids, bile acids, drugs, many other endogenous xenobiotics. UGT8, contrast, uses galactose galactosidate ceramide, an important step synthesis of glycosphingolipids cerebrosides. function fourth family, UGT3, is unknown. Here we report...
The lacrimal gland (LG) develops through branching morphogenesis and produces secretions, including tears, that lubricate protect the ocular surface. Despite prevalence of LG disorders such as dry eye, relatively little is known about regulation development. In this study, we show homeobox transcription factor Barx2 highly expressed in conjunctival epithelium, eyelids [lacrimal, harderian (HG), meibomian (MG)] glands necessary for normal eyelid Barx2–/– mice defective morphogenesis, absence...
The human UDP glycosyltransferase (UGT) 3A family is one of three families involved in the metabolism small lipophilic compounds. Members these catalyze addition sugar residues to chemicals, which enhances their excretion from body. UGT1 and UGT2 members primarily use glucuronic acid glucuronidate numerous compounds, such as steroids, bile acids, therapeutic drugs. We showed recently that UGT3A1, first member UGT3 be characterized, unusual using <i>N</i>-acetylglucosamine donor, rather than...
The human UDP glycosyltransferase (UGT) superfamily comprises four families of enzymes that catalyze the addition sugar residues to small lipophilic chemicals. UGT1 and UGT2 use UDP-glucuronic acid, UGT3 UDP-N-acetylglucosamine, UDP-glucose, UDP-xylose conjugate xenobiotics, including drugs endobiotics such as metabolic byproducts, hormones, signaling molecules. This metabolism renders substrate more polar readily excreted from body and/or functionally inactive. fourth UGT family, called...
Abstract Glucuronidation is an enzymatic process that terminally inactivates steroid hormones, including estrogens and androgens, thereby influencing carcinogenesis in hormone-dependent cancers. While drive breast via the estrogen receptor alpha (ERα), androgens play a critical role as prohormones for biosynthesis ligands androgen (AR). In this study, expression regulation of two androgen-inactivating enzymes, UDP-glucuronosyltransferases UGT2B15 UGT2B17, was assessed cancer. large clinical...
Introduction We previously reported that alveolar macrophages from patients with chronic obstructive pulmonary disease (COPD) are defective in their ability to phagocytose apoptotic cells, a similar defect response cigarette smoke. The exact mechanisms for this unknown. Sphingolipids including ceramide, sphingosine and sphingosine-1-phosphate (S1P) involved diverse cellular processes we hypothesised comprehensive analysis of system COPD may help delineate the reasons phagocytic function....
Abstract In humans, the lacrimal gland (LG) is primary contributor to aqueous layer of tear film. Production tears in insufficient quantity or inadequate quality may lead aqueous-deficiency dry eye (ADDE). Currently there no cure for ADDE. The development strategies reliably isolate LG stem/progenitor cells from tissue brings great promise design cell replacement therapies patients with We analyzed therapeutic potential epithelial progenitor (EPCPs) isolated adult wild-type mouse LGs by...
Genes involved in drug absorption, distribution, metabolism, and excretion (ADME) are called ADME genes. Currently, 298 genes that encode phase I II metabolizing enzymes, transporters, modifiers designated as by the PharmaADME Consortium. highly expressed liver their levels can be influenced diseases such hepatocellular carcinoma (HCC). In this study, we obtained RNA-sequencing microRNA (miRNA)-sequencing data from 371 HCC patients via The Cancer Genome Atlas project performed gene–targeted...
Introduction Sex and gender influence pharmacotherapy outcomes, including adverse drug effects which are nearly twice as common in women. differences responses involve factors diverse body composition, physiology, prescribing patterns. Many drugs show higher exposure women, can be partly attributed to sex-differences processes that control disposition such metabolism transport.
The cell adhesion molecule L1 regulates axonal guidance and fasciculation during development. We previously identified the regulatory region of gene showed that it was sufficient for establishing neural pattern expression in transgenic mice. In present study, we characterize a DNA element within this called HPD contains binding motifs both homeodomain Pax proteins responds to signals from bone morphogenetic (BMPs). An ATTA sequence core required protein Barx2 while separate paired domain...
Among the many factors involved in regulation of chondrogenesis, bone morphogenetic proteins (BMPs) and members Sox homeobox transcription factor families have been shown to crucial roles. Of these regulators, that function during chondrogenesis least well defined. We show here Barx2 is expressed primary mesenchymal condensations, digital rays, developing joints articular cartilage limb, suggesting it plays a role chondrogenesis. Using retroviruses antisense oligonucleotides manipulate...
ABSTRACT Background and objective We previously showed that alveolar macrophages from COPD patients are defective in their ability to phagocytose apoptotic cells (‘efferocytosis’) this defect is potentially linked the sphingosine‐1 phosphate ( S1P ) system, particular receptor 5 S1PR5 ). In patients, mRNA expression levels increased were correlated with both lung function efferocytosis. However, it us unknown whether these changes under epigenetic control via DNA methylation or directly...
Fibroblast growth factor 10 (FGF10) is involved in eye, meibomian, and lacrimal gland (LG) development, but its function adult eye structures remains unknown. This study aimed to characterize the role of FGF10 homeostasis regeneration LG corneal epithelium proliferation.Quantitative reverse transcription PCR was used for analysis expression both early postnatal mouse LG, RNA sequencing analyze gene during inflammation. injected into two models Sjögren's syndrome healthy controls. Flow...
Prx1 and Prx2 are homeobox transcription factors expressed during vasculogenesis. To begin to elucidate how regulated function in the adult vasculature, situ hybridization studies were performed. mRNAs not detected normal rat pulmonary arteries; however, both genes induced with vascular disease, colocalizing sites of tenascin-C (TN-C) expression. Because catabolism extracellular matrix (ECM) is a critical step development we investigated whether changes smooth muscle cell (SMC)–ECM...