A5101406209- Estrogen and related hormone effects
- Prostate Cancer Treatment and Research
- Pharmacogenetics and Drug Metabolism
- FOXO transcription factor regulation
- Protease and Inhibitor Mechanisms
- Receptor Mechanisms and Signaling
- HER2/EGFR in Cancer Research
- Cancer Cells and Metastasis
- Neurotransmitter Receptor Influence on Behavior
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Cancer and Skin Lesions
- Family and Patient Care in Intensive Care Units
- Breast Lesions and Carcinomas
- Cancer, Hypoxia, and Metabolism
- Inflammatory mediators and NSAID effects
- Treatment of Major Depression
- Geriatric Care and Nursing Homes
- Prostate Cancer Diagnosis and Treatment
- Neuropeptides and Animal Physiology
- Interprofessional Education and Collaboration
- Cytokine Signaling Pathways and Interactions
- Computational Drug Discovery Methods
- Breast Cancer Treatment Studies
- Patient Dignity and Privacy
Mental Health Research UK
2022
The Alfred Hospital
2019
Cancer Research UK
2012-2018
University of Cambridge
2011-2018
Edgewood College
2017
Cancer Research UK Cambridge Center
2016
Australian Catholic University
2014
Mayo Clinic in Florida
2006-2012
Mayo Clinic
2006-2012
Sylvester Comprehensive Cancer Center
2012
Estrogen receptor-α (ER) is the driving transcription factor in most breast cancers, and its associated proteins can influence drug response, but direct methods for identifying interacting have been limited. We purified endogenous ER using an approach termed RIME (rapid immunoprecipitation mass spectrometry of proteins) discovered interactome under agonist- antagonist-liganded conditions cancer cells, revealing transcriptional networks cancer. The estrogen-enriched interactor GREB1, a...
Breast and prostate cancer research to date has largely been predicated on the use of cell lines in vitro or vivo . These limitations have led development more clinically relevant models, such as organoids murine xenografts that utilize patient‐derived material; however, issues related low take rate, long duration establishment, associated costs constrain these models. This study demonstrates ex culture freshly resected breast tumor specimens obtained from surgery, termed explants ( PDE s),...
Matrix metalloproteinase 10 (MMP-10, stromelysin-2) is a secreted with functions in skeletal development, wound healing, and vascular remodeling; its overexpression also implicated lung tumorigenesis tumor progression. To understand the regulation of MMP-10 by tissue inhibitors metalloproteinases (TIMPs), we have assessed equilibrium inhibition constants (Ki) putative physiological TIMP-1 TIMP-2 for active catalytic domain human (MMP-10cd) using multiple kinetic approaches. We find that...
Hormonally regulated breast and prostate cancers are the most common cause of cancer in females males respectively. FoxA1 acts as a pioneer factor for both androgen receptor (AR) estrogen receptor-α (ER), dictating binding location, therefore function these transcription factors. It is an essential protein transcriptional activity ER AR, yet it has distinct roles with two different nuclear receptors. In malignancies, plays pivotal role from early stage through to drug resistant metastatic...
Abstract Glucuronidation is an enzymatic process that terminally inactivates steroid hormones, including estrogens and androgens, thereby influencing carcinogenesis in hormone-dependent cancers. While drive breast via the estrogen receptor alpha (ERα), androgens play a critical role as prohormones for biosynthesis ligands androgen (AR). In this study, expression regulation of two androgen-inactivating enzymes, UDP-glucuronosyltransferases UGT2B15 UGT2B17, was assessed cancer. large clinical...
The forkhead protein, FOXA1, is a critical interacting partner of the nuclear hormone receptors, oestrogen receptor-α (ER) and androgen receptor (AR), which are major drivers two most common cancers, namely breast prostate cancer. Over past few years, progress has been made in our understanding how FOXA1 influences function, with both distinct roles regulation ER or AR. Recently, another level described, discovery that mutated 1.8% 3-5% cancers. In addition, subset cancer types exhibit...
Excess proteolytic activity of matrix metalloproteinases (MMPs) contributes to the development arthritis, cardiovascular diseases and cancer progression, implicating these enzymes as therapeutic targets. While many small molecule inhibitors MMPs have been developed, clinical uses limited, in part by toxicity off-target effects. Development endogenous tissue (TIMPs) recombinant biopharmaceuticals represents an alternative approach; however, short plasma half-life TIMPs has restricted their...
Due to the putative involvement of dopaminergic circuits in depression, triple reuptake inhibitors are being developed as a new class antidepressant, which is hypothesized produce more rapid onset and better efficacy than current antidepressants selective for serotonin or norepinephrine neurotransmission. (<i>1S,2S</i>)-3-(Methylamino)-2-(naphthalen-2-yl)-1-phenylpropan-1-ol (PRC200-SS), inhibitor, potently bound human serotonin, norepinephrine, dopamine transporters with...
The amyloid precursor protein (APP) is a ubiquitously expressed transmembrane adhesion and the progenitor of amyloid-beta peptides. major splice isoforms APP by most tissues contain Kunitz protease inhibitor domain; secreted containing this domain also known as nexin 2 potently inhibits serine proteases, including trypsin coagulation factors. atypical human isoform mesotrypsin resistant to inhibition inhibitors cleaves some at substantially accelerated rate. Here, in proteomic screen...
High GATA2 expression has been associated with an increased risk of poor clinical outcomes after radical prostatectomy; however, this not studied in relation to biochemical recurrence (BCR) salvage radiation therapy (SRT) for recurrent prostate cancer prostatectomy. Our aim was evaluate the association between protein levels primary tumour samples and BCR SRT.109 males who were treated SRT included. The percentage cells nuclear staining intensity both measured. These two measures multiplied...
Background Standardly collected clinical and pathological patient information has demonstrated only moderate ability to predict risk of biochemical recurrence (BCR) prostate cancer in men undergoing salvage radiation therapy (SRT) for a rising PSA after radical prostatectomy (RP). Although elevated FOXA1 staining been associated with poor outcomes following RP, it not studied the specific setting SRT RP. The aim this study was evaluate association between level BCR recurrent cancer. Methods...