A5041052887- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Computational Drug Discovery Methods
- Alzheimer's disease research and treatments
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Stress Responses and Cortisol
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Protein Tyrosine Phosphatases
- Nuclear Receptors and Signaling
- Mitochondrial Function and Pathology
- Reproductive System and Pregnancy
- Poxvirus research and outbreaks
- Muscle Physiology and Disorders
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Autophagy in Disease and Therapy
- Galectins and Cancer Biology
National Research Institute for Veterinary Virology and Microbiology of Russia
2021-2023
Ekaterinburg Research Institute of Viral Infections
2022-2023
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms
1997-2018
Maastricht University
2010-2012
European Graduate School of Neuroscience
2010
Maastricht University Medical Centre
2010
University of Liège
2006-2008
Sanford Burnham Prebys Medical Discovery Institute
2002-2003
Cancer Research Center
2001-2003
Engelhardt Institute of Molecular Biology
1999-2002
A cDNA encoding a novel, human, dual-specific protein phosphatase was identified in the Incyte data base. The open reading frame predicted of 184 amino acids related to the<i>Vaccinia</i> virus VH1 and human VH1-related (VHR) phosphatases. Expression VHR-related MKPX (VHX) highest thymus, but also detectable monocytes lymphocytes. VHX-specific antiserum detected with an apparent molecular mass 19 kDa many cells, including T lymphocytes monocytes. VHX expression not induced by cell...
Two new vectors, pAC28 and pEGST, for the co-expression of recombinant genes in E. coli were developed. This two-plasmid system allows an efficient expression purification large amounts protein-protein complexes formed bacterial cells. We have utilized this to express isolate a stable complex two human proteins, hematopoietic cell tyrosine phosphatase (HePTP) mitogen-activated proteins kinase Erk2. approach is useful biochemical structural studies interactions.
Mutant ubiquitin (UBB+1) accumulates in the hallmarks of tauopathies and polyglutamine diseases. We show that deubiquitinating enzyme YUH1 Saccharomyces cerevisiae its mouse human ortholog UCH-L3 are able to hydrolyze C-terminal extension UBB+1. This yields another dysfunctional molecule (UBG76Y) with biochemical properties similar full length UBB+1 may be detected post-mortem tissue due impaired truncation Although level protein several neurodegenerative diseases is unchanged, we vitro...
African swine fever (ASF) is an emerging disease threat to the industry worldwide. There no vaccine against ASF, and progress hindered by a lack of knowledge concerning extent ASFV strain diversity viral antigens conferring type-specific protective immunity in pigs. We have previously demonstrated that homologous serotype-specific proteins CD2v (EP402R) and/or C-type lectin are required for protection challenge with virulent Congo (Genotype I, Serogroup 2), we identified T-cell epitopes on...
In the present study, we investigate mechanism for protein kinase A (PKA)-mediated activation of C-terminal Src (Csk). Although isolated Csk domain was phosphorylated at Ser(364) by PKA to same stoichiometry as wild-type Csk, significant observed only in presence purified homology 3 (SH3 domain). Furthermore, interaction between SH3 and domains facilitated PKA-mediated phosphorylation domain, evaluated surface plasmon resonance. This suggests that an overall structural organization are...
The etiology of the sporadic form Alzheimer's disease (AD) remains largely unknown. Recent evidence has suggested that gene-environment interactions (GxE) may play a crucial role in its development and progression. Whereas various susceptibility loci have been identified, like apolipoprotein E4 allele, these cannot fully explain increasing prevalence AD observed with aging. In addition to such genetic risk factors, environmental factors proposed alter developing as well affect rate cognitive...
African swine fever (ASF) is an infectious disease that causes the most significant losses to pig industry. One of effective methods for combating this could be development vaccines. To date, experimental vaccines based on use live attenuated strains ASF virus (ASFV) obtained by deletion viral genes responsible virulence are effective. Deletion EP402R gene encoding a CD2-like protein led attenuation various ASFV, although degree varies among different isolates. Here we have shown from genome...
Significant amounts (10–30%) of 3′-extended products with one or two extra nucleotides are synthesized in the course run-off tRNA gene transcription T7 RNA polymerase. Denaturing polyacrylamide gel electrophoresis appeared to be insufficient provide preparative pure correct-size transcripts. Formation dimers by transcripts as side their activation is also another obstacle preparation biologically active Here, we have shown that EF-Tu affinity chromatography and/or non-denaturing simple and...
Primary structures of phage T5- and Escherichia coli-encoded tRNA(Phe) are distinct at four out 11 positions known as identity elements for E. coli phenylalanyl-tRNA synthetase (FRS). In order to reveal structural requirements FRS recognition, aminoacylation wild-type T5 gene transcript mutants containing substitutions the 20, 34, 35 36 was compared with transcript. The can be aminoacylated same catalytic efficiency counterpart. However, maximal rate transcripts reached different Mg2+...
African swine fever (ASF) is a contagious disease of pigs caused by the ASF virus (ASFV). The main problem in field control lack vaccines. Attempts to obtain vaccines attenuating ASFV on cultured cell lines led production attenuated viruses, some which provided protection against infection with homologous virus. Here we report biological and genomic features Congo-a (KK262) compared its virulent homologue Congo-v (K49). Our results showed differences vivo replication virulence Congo-a....
African swine fever is a deadly disease of pigs caused by the large DNA virus (ASFV). Despite intensive research, little known about molecular mechanisms ASFV pathogenesis. Transcriptome analysis host and viral genes in infected macrophages revealed changes expression involved various biological processes, including immune response, inflammatory response apoptosis. To understand pathogenesis, we used transcriptome to identify differences gene between peripheral blood monocytes (PBMCs)...
Kinetic parameters of aminoacylation by E. coli phenylalanyl-tRNA synthetase vary for phage T5 tRNA(Phe) gene transcript from 0.950 to 2.545 microM Km and 550 400 min(-1) kcat. To reveal the source this variability various RNA preparations, homogeneity transcripts has been examined. Presence 3' extensions dimer formation in preparations reduced catalytic efficiency kcat/Km several-fold. We have shown that proportion dimers 3'-extended tRNA is sensitive single-base substitutions tRNA. While...
Swinepox virus (SWPV) is the only member of Suipoxvirus genus Poxviridae family and an etiologic agent a worldwide disease specific for domestic wild pigs. SWPV outbreaks are sporadically recorded in different regions Russia. In 2013, outbreak causing skin lesions was registered on pig farm The presence scab samples assessed by in-house real-time PCR, reference PCR amplification, nucleotide sequencing viral late transcription factor-3 (VLTF-3) gene then confirmed isolation. Thus, proposed...