A5037202169- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Renal function and acid-base balance
- Fluoride Effects and Removal
- Physiological and biochemical adaptations
- Heavy Metal Exposure and Toxicity
- Protein Kinase Regulation and GTPase Signaling
- Electrolyte and hormonal disorders
- Neurobiology and Insect Physiology Research
- Ion Channels and Receptors
- Magnesium in Health and Disease
- Nutritional Studies and Diet
- Liver Disease Diagnosis and Treatment
- Sphingolipid Metabolism and Signaling
- Drug Transport and Resistance Mechanisms
- Sodium Intake and Health
- Molecular Sensors and Ion Detection
- Birth, Development, and Health
- Hormonal Regulation and Hypertension
- Effects and risks of endocrine disrupting chemicals
- Parathyroid Disorders and Treatments
- Zebrafish Biomedical Research Applications
- Health, Environment, Cognitive Aging
- Air Quality and Health Impacts
New York University
2024
Instituto Nacional de Cardiología
2013-2023
Instituto Nacional de Salud Pública
1996-2022
Instituto Nacional de Cardiologia
2019-2020
Instituto Nacional de Salud
2018
Case Western Reserve University
2006-2016
University of Nicosia
2016
Weatherford College
2016
Cornell University
2016
Autonomous University of Aguascalientes
2015
The K+-Cl- cotransporters (KCCs) belong to the gene family of electroneutral cation-chloride cotransporters, which also includes two bumetanide-sensitive Na+-K+-2Cl- and a thiazide-sensitive Na+-Cl- cotransporter. We have cloned cDNAs encoding mouse KCC3, human KCC4, three new members this family. KCC3 KCC4 predict proteins 1083 1150 amino acids, respectively. are 65-71% identical previously characterized transporters KCC1 KCC2, with they share predicted membrane topology. four KCC differ at...
We report the molecular and functional characterization of murine Slc26a6, putative apical chloride-formate exchanger proximal tubule. The Slc26a6 transcript is expressed in several tissues, including kidney. Alternative splicing second exon generates two distinct isoforms, denoted Slc26a6a Slc26a6b, which differ inclusion a 23-residue NH 2 -terminal extension. Functional comparison with Slc26a1, basolateral oxalate tubule, reveals number intriguing differences. Whereas capable Cl − ,...
The K<sup>+</sup>-Cl<sup>−</sup>cotransporters (KCCs) are members of the cation-chloride cotransporter gene family and fall into two phylogenetic subgroups: KCC2 paired with KCC4 KCC1 KCC3. We report a functional comparison in <i>Xenopus</i> oocytes KCC4, widely expressed representatives these subgroups. exhibit differential sensitivity to transport inhibitors, such that is much less sensitive bumetanide furosemide. efficacy anion inhibitors critically dependent on concentration...
GABA- and glycine-induced depolarization is thought to provide important developmental signals, but the role of underlying chloride gradient has not been examined from onset development. We therefore overexpressed globally potassium-chloride cotransporter 2 (KCC2) in newly fertilized zebrafish embryos reverse gradient. This rendered glycine hyperpolarizing all neurons, tested at time that motor behaviors (but native KCC2) first appear. KCC2 overexpression resulted fewer mature spontaneously...
Aim: We examined the association between birth weight and methylation in imprinted IGF/H19 loci, nonimprinted gene NR3C1 repetitive element DNA (LINE-1 Alu). Materials & methods: collected umbilical cord venous blood from 219 infants born Mexico City (Mexico) as part of a prospective cohort study analyzed using pyrosequencing. Results: Birth was not associated with regions studied. One CpG dinucleotides IGF2 imprinting control region (ICR)1 includes potential C–T SNP. Among individuals an...
Uric acid, generated from the metabolism of purines, has both proven and emerging roles in human disease. Serum uric acid humans is determined by production net balance reabsorption secretion kidney intestine. In kidney, epithelial dominates over secretion, such that normal subjects there at least 90% filtered urate resulting a fractional excretion <10%. Tranilast, an anti-inflammatory drug with pleiotropic effects, marked hypouricemic, uricosuric effect humans. We report here tranilast...
Studies have suggested that plasma lead levels may better reflect the toxicologically labile fraction of circulatory Pb is more freely available for exchange with target tissues than do in whole blood. also reported an apparent severalfold variation relative partitioning between blood and (or serum) a given whole-blood level. This inherent differences Pb/whole among individuals and/or methodologic challenges associated collection analyses samples generally contain < 1-2 ng total Pb. Here, we...
The neuron-specific K(+)-Cl(-) cotransporter KCC2 plays a crucial role in determining intracellular chloride activity and thus the neuronal response to gamma-aminobutyric acid glycine. Of four KCCs, is unique mediating constitutive cotransport under isotonic conditions; other three KCCs are exclusively swelling-activated, with no activity. We have utilized series of chimeric cDNAs localize determinant transport KCC2. Two generations KCC4-KCC2 initially localized this characteristic within...
The SLC12A6 gene encoding the K + -Cl − cotransporter KCC3 is expressed in multiple tissues, including kidney. Here, we report molecular characterization of several NH 2 -terminal isoforms human and mouse KCC3, along with intrarenal localization functional Xenopus laevis oocytes. Two major isoforms, KCC3a KCC3b, are generated by transcriptional initiation 5′ two distinct first coding exons. Northern blot analysis tissues indicates that KCC3b expression particularly robust kidney, which also...
KCC2 is the neuron-specific member of K<sup>+</sup>-Cl<sup>−</sup> cotransporter gene family. It also only its family that active under physiologically normal conditions, in absence osmotic stress. By extruding Cl<sup>−</sup> from neuron isotonic this transporter maintains a low concentration neuronal Cl<sup>−</sup>, which essential for fast inhibitory synaptic transmission by GABA and glycine mature nervous system. The other members are exclusively swelling-activated. Here we demonstrate 15...
The K(+):Cl(-) cotransporter (KCC) activity is modulated by phosphorylation/dephosphorylation processes. In isotonic conditions, KCCs are inactive and phosphorylated, whereas hypotonicity promotes their dephosphorylation activation. Two phosphorylation sites (Thr-991 Thr-1048) in KCC3 have been found to be critical for its regulation. However, here we show that the double mutant KCC3-T991A/T1048A could further activated hypotonicity, suggesting additional site(s) involved. We observed vitro...
K(+)-Cl(-) cotransporter (KCC) isoforms 3 (KCC3) and 4 (KCC4) are expressed at the basolateral membrane of proximal convoluted tubule cells, KCC4 is present in thick ascending loop Henle's limb α-intercalated cells collecting duct. Little known, however, about physiological roles these transporters kidney. We evaluated KCC3 mRNA protein expression levels intrarenal distribution male Wistar rats or C57 mice under five experimental conditions: hyperglycemia after a single dose streptozotocin,...
Sources of fluoride exposure for Mexicans include foods, beverages, fluoridated salt, and naturally water. There are no available data describing content foods beverages consumed in Mexico.To measure the typically to compare their that those from United States Kingdom.Foods reported as part Mexican Health Nutrition Survey (n = 182) were purchased largest supermarket chains local markets Mexico City. Samples analyzed fluoride, at least duplicate, using a modification hexamethyldisiloxane...
K(+)Cl(-) cotransporters (KCCs) play fundamental physiological roles in processes such as inhibitory neurotransmission and cell volume regulation. Mammalian genomes encode four distinct KCC paralogs, which share basic transport characteristics but differ significantly ion affinity, pharmacology, relative sensitivity to volume. Studies identify divergence functional have thus far focused on the cytoplasmic termini. Here, we investigated sequence requirements of large extracellular loop (LEL)...
Foods and beverages provide a source of fluoride exposure in Mexico. While high concentrations are neurotoxic, recent research suggests that exposures within the optimal range may also pose risk to developing brain. This prospective study examined whether dietary intake during pregnancy is associated with toddlers' neurodevelopment 103 mother-child pairs from PROGRESS cohort Mexico City. Food beverage was assessed trimesters 2 3 using food frequency questionnaire Mexican tables content. We...
Sulfate is required for proper cell growth and development of all organisms. We have shown that the renal sulfate transport system has dual roles in euryhaline eel, namely, maintenance homeostasis osmoregulation body fluids. To clarify physiological transporters teleost fish, we cloned orthologs mammalian Slc13a1 (NaSi-1) Slc26a1 (Sat-1) from eel (Anguilla japonica) assessed their functional characteristics, tissue localization, regulated expression. Full-length cDNAs coding ajSlc13a1...