A5074979435- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Cancer Research and Treatments
- Amoebic Infections and Treatments
- Cancer-related molecular mechanisms research
- Aquaculture disease management and microbiota
- Ion Transport and Channel Regulation
- MicroRNA in disease regulation
- Hormonal Regulation and Hypertension
- Renin-Angiotensin System Studies
- Immune Response and Inflammation
- Aquaculture Nutrition and Growth
- Plant Disease Resistance and Genetics
- Chemical synthesis and alkaloids
- Salmonella and Campylobacter epidemiology
- Genetic diversity and population structure
- Plant Pathogens and Fungal Diseases
- Sodium Intake and Health
- Pancreatic function and diabetes
- Fungal Biology and Applications
- Gene expression and cancer classification
- Microbial Metabolic Engineering and Bioproduction
- Species Distribution and Climate Change
Universidad de Málaga
2016-2025
Instituto de Investigación Biomédica de Málaga
2021-2025
Hospital Regional Universitario de Málaga
2022
Instituto Nacional de Cardiología
2009-2021
Andalusian Centre for Nanomedicine and Biotechnology
2013-2019
Mexican Social Security Institute
2016
Institute of Bioinformatics
2015
Hospital General de México
2014
Weatherford College
2013
American Society of Nephrology
2013
The recent advent of long read sequencing technologies, such as Pacific Biosciences (PacBio) and Oxford Nanopore technology (ONT), have led to substantial improvements in accuracy computational cost genomes. However, de novo whole-genome assembly still presents significant challenges related the quality results. Pursuing remains a formidable challenge, underscored by intricate considerations surrounding demands result quality. As throughput steadily advance, continuous stream innovative...
In spite of the biological and economic importance plants, relatively few plant species have been sequenced. Only genome sequence plants with small genomes, most them angiosperms, in particular eudicots, has determined. The arrival next-generation sequencing technologies allowed rapid efficient development new genomic resources for non-model or orphan species. But pace is far from that animals microorganisms. This review focuses on typical challenges genomes can explain why genomics less...
Summary Maritime pine ( P inus pinaster A it.) is a widely distributed conifer species in S outhwestern E urope and one of the most advanced models for research. In current work, comprehensive characterization maritime transcriptome was performed using combination two different next‐generation sequencing platforms, 454 I llumina. De novo assembly provided catalogue 26 020 unique transcripts trees collection 9641 full‐length c DNA s. Quality validated by RT ‐ PCR amplification selected...
Ripening is a complex physiological process that involves changes in reactive nitrogen and oxygen species govern the shelf-life quality of fruits. Nitric oxide (NO)-dependent sweet pepper fruit transcriptome were determined by treating fruits at initial breaking point stage with NO gas. Fruits also harvested immature (green) ripe (red) stages. Fruit ripening absence resulted abundance 8805 transcripts whose function could be identified. Among these, functional clusters associated...
The hyperglycemia triggers several chronic diabetic complications mediated by increased oxidative stress that eventually causes nephropathy. aim of this study was to examine if the sodium-glucose cotransporter (SGLT2) inhibition prevents in kidney rats. Methods . rat model established intraperitoneal injection streptozotocin (50 mg/kg). SGLT2 induced daily subcutaneous administration phlorizin (0.4 g/kg). Oxidative assessed catalase (CAT), glutathione peroxidase (GPx), and superoxide...
Renal immune cell infiltration and cells expressing angiotensin II (AII) in tubulointerstitial areas of the kidney are features experimental models salt-sensitive hypertension (SSHTN). A high-salt intake tends to suppress circulating AII levels, but intrarenal concentrations have not been investigated SSHTN. This study explored relationship between these gain insight into pathophysiology Plasma renal interstitial (microdialysis technique) macrophages, lymphocytes, AII-positive were...
Senegalese sole (Solea senegalensis) and common (S. solea) are two economically evolutionary important flatfish species both in fisheries aquaculture. Although some genomic resources tools were recently described these species, further sequencing efforts required to establish a complete transcriptome, identify new molecular markers. Moreover, the comparative analysis of transcriptomes will be useful understand evolution. A comprehensive characterization transcriptome for each was carried out...
Immune cell infiltration of the kidney is a constant feature in salt-sensitive hypertension (SSHTN). We evaluated relationship between renal inflammation and pressure natriuresis model SSHTN that results from transient oral administration N(ω)-nitro-L-arginine methyl ester (L-NAME). Pressure was determined Wistar rats received 4 wk high-salt (4% NaCl) diet, starting 1 after stopping L-NAME, which administered alone (SSHTN group, n = 17) or association with mycophenolate mofetil (MMF; MMF...
Objectives: Insulin is recognized to increase renal salt reabsorption in the distal nephron and hyperinsulinemic states have been shown be associated with increased expression of NaCl cotransporter (NCC). However, effect insulin on NCC functional activity has not reported. Methods: Using a heterologous system Xenopus laevis oocytes, mouse convoluted cell line, mDCT15 cells, endogenously expressing NCC, an ex-vivo kidney perfusion technique, we assessed phosphorylation NCC. The signaling...
Abstract Background Transcript profiling of differentiating secondary xylem has allowed us to draw a general picture the genes involved in wood formation. However, our knowledge is still limited about regulatory mechanisms that coordinate and modulate different pathways providing substrates during xylogenesis. The development compression conifers constitutes an exceptional model for these studies. Although differential expression few compared normal or opposite been reported, broad range...
Macrophage migration inhibitory factor (MIF) exerts either a protective or deleterious role in the immune response to different pathogens. We analyzed herein of MIF host control toxoplasmosis using MIF(-/-) mice backcrossed BALB/c C57BL/6 genetic backgrounds. Both, wild-type (WT) and were susceptible infection with highly virulent RH as well moderately ME49 strains T. gondii. mice, however, showed greater liver damage more brain cysts, produced less proinflammatory cytokines, succumbed...
Pinus pinaster is an economically and ecologically important species that becoming a woody gymnosperm model. Its enormous genome size makes whole-genome sequencing approaches are hard to apply. Therefore, the expressed portion of has be characterised results annotations have stored in dedicated databases.EuroPineDB largest sequence collection available for single pine species, (maritime pine), since it comprises 951 641 raw reads obtained from non-normalised cDNA libraries high-throughput...
Faba bean is an important food crop worldwide. However, progress in faba genomics lags far behind that of model systems due to limited availability genetic and genomic information. Using the Illumina platform transcriptome from leaves two lines (29H Vf136) subjected Ascochyta fabae infection have been characterized. De novo assembly provided a total 39,185 different transcripts were functionally annotated, among these, 13,266 assigned gene ontology against Arabidopsis. Quality was validated...
Background Hypercalciuria can result from activation of the basolateral calcium-sensing receptor (CaSR), which in thick ascending limb Henle's loop controls Ca2+ excretion and NaCl reabsorption response to extracellular However, function CaSR regulation distal convoluted tubule (DCT) is unknown. We hypothesized that this location involved activating thiazide-sensitive cotransporter (NCC) prevent loss.Methods used a combination vitro vivo models examine effects on NCC activity. Because...
The cucurbit powdery mildew elicited by Podosphaera xanthii is one of the most important limiting factors in production. Our knowledge genetic and molecular bases underlying physiological processes governing this disease very limited. We used RNA-sequencing to identify differentially expressed genes leaves Cucumis melo upon inoculation with P. xanthii, using RNA samples obtained at different time points during early stages infection their corresponding uninfected controls. In parallel, melon...
Nowadays, Huanglongbing (HLB) disease, associated with Candidatus Liberibacter asiaticus (CLas), seriously affects citriculture worldwide, and no cure is currently available. Transcriptomic analysis of host–pathogen interaction the first step to understand molecular landscape a disease. Previous works have reported transcriptome profiling in response HLB different susceptible citrus species; however, similar studies tolerant species, including Mexican lime, are limited. In this work, we...
Glucose uptake is increased in hypertension. Thus we investigated Na + -glucose cotransporter (SGLT2) activity and expression proximal tubules from renovascular hypertensive rats. Sham-operated rats, aortic coarctation rats treated with either ramipril (2.5 mg·kg -1 ·day for 21 days) or losartan (10 were used. -dependent glucose was measured brush-border membrane vesicles (BBMV). V max BBMV greater compared those normotensive (3 ± 0.2 vs. 1.5 0.1 nmol·mg protein ·min ) without a change K m ....
K(+)-Cl(-) cotransporter (KCC) isoforms 3 (KCC3) and 4 (KCC4) are expressed at the basolateral membrane of proximal convoluted tubule cells, KCC4 is present in thick ascending loop Henle's limb α-intercalated cells collecting duct. Little known, however, about physiological roles these transporters kidney. We evaluated KCC3 mRNA protein expression levels intrarenal distribution male Wistar rats or C57 mice under five experimental conditions: hyperglycemia after a single dose streptozotocin,...
Infected pork meat is an important source of non-typhoidal human salmonellosis. Understanding molecular mechanisms involved in disease pathogenesis for the development therapeutic and preventive strategies. Thus, hereby we study transcriptional profiles along porcine intestine during infection with Salmonella Typhimurium, as well post-transcriptional gene modulation by microRNAs (miRNA). Sixteen piglets were orally challenged S. Typhimurium. Samples from jejunum, ileum colon, collected 1, 2...
The cucurbit powdery mildew fungus Podosphaera xanthii is a major limiting factor for production worldwide. Despite the fungus's agronomic and economic importance, very little known about fundamental aspects of P. biology, such as obligate biotrophy or pathogenesis. To design more durable control strategies, genomic information needed. Powdery mildews are fungal pathogens with large genomes compared those other fungi, which contain vast amounts repetitive DNA sequences, much composed...
The host response to S. Typhimurium infection can be post-transcriptionally regulated by miRNAs. In this study, we investigated the role of miR-215 using both in vivo porcine models and vitro intestinal epithelial cell lines. Several miRNAs were found dysregulated ileum during with wild-type SPI2-defective mutant strains Typhimurium, some changes being SPI2-dependent. Notably, was significantly downregulated infection. To explore its functional role, gain-of-function experiments performed...
Transient administration of ANG II causes persistent salt-sensitive hypertension associated with arteriolopathy, interstitial inflammation, and cortical vasoconstriction; blocking the vascular inflammatory changes mycophenolate mofetil (MMF) prevents vasoconstriction. While infiltrating leukocytes during phase express II, functional role this is not known. We examined acute effect candesartan on renal hemodynamics established hypertensive related these findings to direct measurement...