A5024499260- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Atmospheric chemistry and aerosols
- Genetic diversity and population structure
- Bioenergy crop production and management
- Plant Disease Resistance and Genetics
- Metabolism, Diabetes, and Cancer
- Plant Micronutrient Interactions and Effects
- Genomics and Phylogenetic Studies
- Plant responses to elevated CO2
- Plant Reproductive Biology
Universidad de Málaga
2008-2017
Summary Maritime pine ( P inus pinaster A it.) is a widely distributed conifer species in S outhwestern E urope and one of the most advanced models for research. In current work, comprehensive characterization maritime transcriptome was performed using combination two different next‐generation sequencing platforms, 454 I llumina. De novo assembly provided catalogue 26 020 unique transcripts trees collection 9641 full‐length c DNA s. Quality validated by RT ‐ PCR amplification selected...
Summary The PpDof5 transcription factor from maritime pine ( Pinus pinaster) is a regulator of the expression glutamine synthetase (GS) genes in photosynthetic and non‐photosynthetic tissues. mRNA detected almost ubiquitously during development with low levels gene green tissues much higher roots lignified shoots. protein expressed bacteria binds to oligonucleotide probes containing AAAG core sequence derived promoters GS1a GS1b genes. Transient experiments agroinfiltrated tobacco leaves...
Asparagine, a key amino acid for nitrogen storage and transport in plants, is synthesized via the ATP-dependent reaction catalyzed by enzyme asparagine synthetase (AS; EC 6.3.5.4). In this work, we present molecular analysis of two full-length cDNAs that encode maritime pine (Pinus pinaster Ait.), PpAS1 PpAS2. Phylogenetic analyses deduced sequences revealed both genes are class II AS, suggesting an ancient origin these plants. A comparative study PpAS2 gene expression profiles showed highly...
Tolerance to ammonium nutrition in plants can be related their ability detoxify via nitrogen assimilation roots. Here, we report that sorghum–sudangrass (Sorghum bicolor L. × S. var. sudanense) hybrids exhibited enhanced biomass production under high levels of inorganic supply as well increased capacity for Glutamine synthetase (GS, EC 6.3.1.2) activity and protein accumulated roots at increasing concentrations either nitrate or ammonium, with particularly GS ammonium-treated plants....
In this work, the role of pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism has been examined poplar trees. The overexpression gene potential effects on growth biomass production were compared between trees growing a chamber under controlled conditions field trial during two seasons. Ten-week-old transgenic poplars exhibited higher than untransformed controls enhanced capacity for inorganic uptake form nitrate. Furthermore, accumulated significantly more carbohydrates...
PpDof 5 is a regulator of the expression glutamine synthetase (GS; EC 6.3.1.2) genes in photosynthetic and non-photosynthetic tissues maritime pine. We have used Arabidopsis thaliana as model system to study function planta, generating transgenic lines overexpressing pine transcription factor. The overexpression resulted substantial increase lignin content with simultaneous regulation carbon nitrogen key genes. In addition, partitioning compounds was spread via various secondary metabolic...