A5073066427- Monoclonal and Polyclonal Antibodies Research
- Genetic diversity and population structure
- Plant Pathogens and Fungal Diseases
- Immunodeficiency and Autoimmune Disorders
- Glycosylation and Glycoproteins Research
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Disease Resistance and Genetics
- Research Data Management Practices
- Forest ecology and management
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Plant and Fungal Interactions Research
- Scientific Computing and Data Management
- Genetic and phenotypic traits in livestock
- Plant Taxonomy and Phylogenetics
- vaccines and immunoinformatics approaches
- Forest Insect Ecology and Management
- Biomedical Text Mining and Ontologies
- Forest Management and Policy
- Chronic Lymphocytic Leukemia Research
- RNA and protein synthesis mechanisms
- Remote Sensing and LiDAR Applications
- Horticultural and Viticultural Research
- Plant Gene Expression Analysis
Centre d'Études Scientifiques et Techniques d'Aquitaine
2013-2025
Université de Bordeaux
2013-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2023-2025
UMR BIOdiversity, GEnes & Communities
2014-2024
Laboratoire d'Informatique Gaspard-Monge
2008-2018
Université de Montpellier
2001-2018
Centre National de la Recherche Scientifique
2001-2018
Institut de Génétique Humaine
2008-2018
Institut de Génétique Moléculaire de Montpellier
2012-2018
Immungenetics (Germany)
2009
IMGT®, the international ImMunoGeneTics information system® (http://www.imgt.org), was created in 1989 by Marie-Paule Lefranc, Laboratoire d'ImmunoGénétique Moléculaire LIGM (Université Montpellier 2 and CNRS) at Montpellier, France, order to standardize manage complexity of immunogenetics data. The building a unique ontology, IMGT-ONTOLOGY, has made IMGT® global reference immunoinformatics. is high-quality integrated knowledge resource specialized immunoglobulins or antibodies, T cell...
Oaks are an important part of our natural and cultural heritage. Not only they ubiquitous in most common landscapes1 but have also supplied human societies with invaluable services, including food shelter, since prehistoric times2. With 450 species spread throughout Asia, Europe America3, oaks constitute a critical global renewable resource. The longevity (several hundred years) probably underlies their emblematic historical importance. Such long-lived sessile organisms must persist the face...
IMGT/3Dstructure-DB is the three-dimensional (3D) structure database of IMGT, international ImMunoGenetics information system that acknowledged as global reference in immunogenetics and immunoinformatics. contains 3D structures immunoglobulins (IG) or antibodies, T cell receptors (TR), major histocompatibility complex (MHC) proteins, antigen receptor/antigen complexes (IG/Ag, TR/peptide/MHC) vertebrates; related proteins immune (RPI) vertebrates invertebrates, belonging to immunoglobulin MHC...
Abstract The 1.5 Gbp/2C genome of pedunculate oak ( Quercus robur ) has been sequenced. A strategy was established for dealing with the challenges imposed by sequencing such a large, complex and highly heterozygous whole‐genome shotgun WGS approach, without use costly time‐consuming methods, as fosmid or BAC clone‐based hierarchical methods. combined short long reads. Over 49 million reads provided Roche 454 GS ‐ FLX technology were assembled into contigs shorter Illumina sequence from...
How temperate forests will respond to climate change is uncertain; projections range from severe decline increased growth. We conducted field tests of sessile oak (Quercus petraea), a widespread keystone European forest tree species, including more than 150 000 trees sourced 116 geographically diverse populations. The were planted on 23 sites in six countries, order expose them wide climates, reflecting future warmer and drier climates. By assessing height survival, our objectives twofold:...
Summary Maritime pine ( P inus pinaster A it.) is a widely distributed conifer species in S outhwestern E urope and one of the most advanced models for research. In current work, comprehensive characterization maritime transcriptome was performed using combination two different next‐generation sequencing platforms, 454 I llumina. De novo assembly provided catalogue 26 020 unique transcripts trees collection 9641 full‐length c DNA s. Quality validated by RT ‐ PCR amplification selected...
INTRODUCTION IMGT/DomainGapAlign is the IMGT online tool for analysis of amino acid (AA) sequences and two-dimensional (2D) structures domains. The use this described here. Three domain types can be analyzed: variable (V) constant (C) domains immunoglobulins (IG) T cell receptors (TR) other immunoglobulin superfamily (IgSF) proteins, groove (G) major histocompatibility (MH) MH (MhSF) proteins. creates gaps in user delimits, V C domains, framework regions (FR-IMGT) complementarity determining...
INTRODUCTION IMGT/3Dstructure-DB is the three-dimensional (3D) structure database of IMGT, international ImMunoGenetics information system that acknowledged as global reference in immunogenetics and immunoinformatics. contains 3D structures (1) antigen receptors comprise immunoglobulins (IG) or antibodies T cell (TR), (2) major histocompatibility (MH) proteins class I (MH1) II (MH2), (3) peptide/MH (pMH) complexes (pMH1, pMH2), (4) receptor/antigen (IG/Ag, TR/pMH), (5) related immune (RPI)...
We developed the densest single-nucleotide polymorphism (SNP)-based linkage genetic map to date for genus Quercus. An 8k gene-based SNP array was used genotype more than 1,000 full-sibs from two intraspecific and interspecific full-sib families of Quercus petraea robur. A high degree collinearity observed between eight parental maps species. composite then established with 4,261 markers spanning 742 cM over 12 groups (LGs) oak genome. Nine genomic regions six LGs displayed highly significant...
Abstract Background The availability of a large expressed sequence tags (EST) resource and recent advances in high-throughput genotyping technology have made it possible to develop highly multiplexed SNP arrays for multi-objective genetic applications, including the construction meiotic maps. Such approaches are particularly useful species with genome size, precluding use whole-genome shotgun assembly current technologies. Results In this study, 12 k-SNP array was developed maritime pine...
INTRODUCTION IMGT/Collier de Perles is the IMGT online tool that allows one to draw standardized two-dimensional (2D) graphical representations of protein domains, or Colliers Perles, starting from user’s own domain amino acid (AA) sequences. The use this described here. can be drawn for three types: variable (V) and constant (C) immunoglobulin (IG) T cell receptor (TR) other members superfamily (IgSF), groove (G) major histocompatibility (MH) MH (MhSF). Sequences have gapped according...
Abstract Maritime pine provides essential ecosystem services in the south‐western M editerranean basin, where it covers around 4 million ha. Its scattered distribution over a range of environmental conditions makes an ideal forest tree species for studies local adaptation and evolutionary responses to climatic change. Highly multiplexed single nucleotide polymorphism ( SNP ) genotyping arrays are increasingly used study genetic variation living organisms practical applications plant animal...
Many northern-hemisphere forests are dominated by oaks. These species extend over diverse environmental conditions and thus interesting models for studies of plant adaptation speciation. The genomic toolbox is an important asset exploring the functional variation associated with natural selection.
An Illumina Infinium SNP genotyping array was constructed for European white oaks. Six individuals of Quercus petraea and Q. robur were considered discovery using both previously obtained Sanger sequences across 676 gene regions (1371 in vitro SNPs) Roche 454 technology from 5112 contigs (6542 putative silico SNPs). The 7913 SNPs genotyped the six parental individuals, full-sib progenies (one within each species two interspecific crosses between robur) three natural populations south-western...
The accessibility of high-throughput genotyping technologies has contributed greatly to the development genomic resources in non-model organisms. High-density arrays have only recently been developed for some economically important species such as conifers. potential using association mapping and breeding depends largely on genome wide patterns diversity linkage disequilibrium current populations. This study aims deepen our knowledge regarding these issues maritime pine, first used...
Abstract Background Descriptive metadata are vital for reporting, discovering, leveraging, and mobilizing research datasets. However, resolving issues as part of a data management plan can be complex producers. To organize document data, various descriptive must created. Furthermore, when sharing it is important to ensure interoperability in line with FAIR (Findable, Accessible, Interoperable, Reusable) principles. Given the practical nature these challenges, there need tools that assist...
Summary Large‐scale tree distribution changes have received considerable attention but underlying demo‐genetic mechanisms are less well documented. We used a diachronic approach to track species shifts in mixed oak stand ( Quercus petraea–Quercus robur ) at fine spatiotemporal scale. Species assignment was made using single nucleotide polymorphism SNP fingerprints employing clustering and parentage analysis. Mating patterns reproductive success were assessed by Plot‐based inventories of soil...
While recent advances have been gained on genome evolution in angiosperm lineages, virtually nothing is known about karyotype the other group of seed plants, gymnosperms. Here we used high density gene-based linkage mapping to compare structure two families conifers (the most abundant gymnosperms) separated around 290 million years ago: Pinaceae and Cupressaceae. We propose for first time a model based fusion 20 ancestral chromosomal blocks that may shaped modern karyotpes (with n=12)...
Mass spectrometry (MS) analysis for detection of immunoglobulins (IG) the human IgG3 subclass is described that relies on polymorphic amino acids heavy gamma3 chains. most IgG with thirteen G3m allotypes located constant CH2 and CH3 domains chain, combination which leads to six major alleles. Amino acid changes resulting extensive sequencing previously led definition 19 IGHG3 alleles have been correlated As a proof concept, MS proteotypic peptides were defined encompass discriminatory...