A5007619614- Bone Metabolism and Diseases
- Osteoarthritis Treatment and Mechanisms
- Bone health and treatments
- interferon and immune responses
- Genetic Syndromes and Imprinting
- NF-κB Signaling Pathways
- Cancer-related gene regulation
- Retinoids in leukemia and cellular processes
- TGF-β signaling in diseases
- Epigenetics and DNA Methylation
- Biofield Effects and Biophysics
- Natural product bioactivities and synthesis
- Cytokine Signaling Pathways and Interactions
- Cancer-related Molecular Pathways
- Cell Adhesion Molecules Research
- Electromagnetic Fields and Biological Effects
- Plant biochemistry and biosynthesis
- Histone Deacetylase Inhibitors Research
- Inflammatory mediators and NSAID effects
- Biological Activity of Diterpenoids and Biflavonoids
- Neuroblastoma Research and Treatments
- Fibroblast Growth Factor Research
- Parathyroid Disorders and Treatments
- Alkaline Phosphatase Research Studies
- Connective tissue disorders research
Nagasaki University
2018-2025
University of Nagasaki
2011-2015
Chugoku Electric Power (Japan)
2002
Runx2 and Sp7 are essential transcription factors for osteoblast differentiation. However, the molecular mechanisms responsible proliferation of progenitors remain unclear. The early onset expression caused limb defects through Fgfr1-3 regulation by Runx2. To investigate physiological role in Fgfr1-3, we compared Sp7-/- Runx2-/- mice. Osteoblast accumulated actively proliferated calvariae mandibles but not mice, number their were dependent on gene dosage background. Fgfr2 Fgfr3, which...
Chondrocytes proliferate and mature into hypertrophic chondrocytes. Vascular invasion the cartilage occurs in terminal chondrocyte layer, chondrocytes die by apoptosis or transdifferentiate osteoblasts. Runx2 is essential for osteoblast differentiation maturation. -deficient mice are composed of cartilaginous skeletons lack vascular cartilage. However, requirement cartilage, mechanism transdifferentiation to osteoblasts, its significance bone development remain be elucidated. To investigate...
ABSTRACT Runt-related transcription factor-2 (Runx2) is an essential factor for osteoblast differentiation. However, its functions after the commitment into osteoblasts are controversial and remain to be clarified. We generated enhanced green fluorescent protein (EGFP)-Cre transgenic mice driven by 2.3-kilobase (kb) Col1a1 promoter, Runx2 was deleted in odontoblasts Runx2fl/flCre mice. The sutures fontanelles were more widely opened newborns than Runx2fl/fl newborns. exhibited dwarfism with...
Zinc finger-containing transcription factor Osterix/Specificity protein-7 (Sp7) is an essential for osteoblast differentiation. However, its functions in differentiated osteoblasts remain unclear and the effects of osteoblast-specific
Runx2 (runt related transcription factor 2) is an essential for osteoblast proliferation and differentiation. Uridine diphosphate (UDP)-N-acetylgalactosamine (GalNAc): polypeptide GalNAc-transferase 3 (Galnt3) prevents proteolytic processing of fibroblast growth 23 (Fgf23), which a hormone that regulates the serum level phosphorus. Galnt3 were expressed in osteoblasts osteocytes, Fgf23 expression was restricted to osteocytes bone. Overexpression knock-down upregulated downregulated,...
Runx2 is an essential transcription factor for osteoblast differentiation and chondrocyte maturation. The spaciotemporal expression of regulated by enhancers. We previously identified a 1.3-kb osteoblast-specific enhancer; however, mice with its deletion showed no phenotypes. A 0.8-kb conserved region detected near the enhancer did not exhibit activity in reporter assays, whereas four tandem repeats 452 bp (452×4), containing most 0.8 kb, induced strong cell lines. However, chondrocytes...
Runx2 is an essential transcription factor for osteoblast differentiation and chondrocyte maturation. The spatiotemporal expression of regulated by enhancers. We previously identified a 1.3 kb osteoblast-specific enhancer; however, mice with this deletion showed no phenotypes. A 0.8 conserved region detected near the enhancer did not exhibit activity in reporter assays, whereas four tandem repeats 452 bp (452 × 4) containing most induced strong cell lines. However, chondrocytes enhanced...
An effect on the tumor promotion process, as represented by accelerated cell growth, has been indicated one example of areas that demonstrate possibility biological effects extremely-low frequency magnetic fields. We, therefore, exposed five lines (HL-60, K-562, MCF-7, A-375, and H4) derived from human tumors to a field for 3 days investigate growth. Prior exposure or sham exposure, cells were precultured 2 in low serum conditions. The number growing was counted blind manner. To initial...
Geranylgeranoic acid (GGA) and its derivatives are currently under development as chemopreventive agents against second primary hepatoma in Japan. We aimed to evaluate chemoprevention targets of GGA a surrogate marker response clarify the molecular mechanism with GGA. Human hepatoma-derived cell lines such HuH-7, PLC/PRF/5, HepG-2, were treated derivatives. Cellular dynamics several cell-cycle-related proteins assessed by either immunoblotting or immunofluorescence method. The cellular...
Runx2 is required for chondrocyte proliferation and maturation. In the search of target genes in chondrocytes, we found that up-regulated expression hematopoietic cell kinase (Hck), which a member Src tyrosine family, Hck was high cartilaginous limb skeletons wild-type mice but low those Runx2–/– mice, bound promoter region Hck. To investigate functions transgenic expressing constitutively active form (HckCA) were generated using Col2a1 promoter/enhancer. The hind fused, tibia became large,...
Lysine-specific demethylase 1 (LSD1/KDM1A), a histone-modifying enzyme, is upregulated in many cancers, especially neuroblastoma, breast cancer and hepatoma. We have established simple method to measure LSD1 activity using synthetic N-terminal 21-mer peptide of histone H3, which dimethylated at Lys-4 (H3K4me2). After the enzyme reaction, substrate H3K4me2 two demethylated products, H3K4me1 H3K4me0, were quantitatively determined by flow injection time-of-flight mass spectrometry (FI-TOF/MS)....