A5103039166- Allergic Rhinitis and Sensitization
- Dermatology and Skin Diseases
- Food Allergy and Anaphylaxis Research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Contact Dermatitis and Allergies
- Transgenic Plants and Applications
- Asthma and respiratory diseases
- Exercise and Physiological Responses
- Pharmacological Effects of Natural Compounds
- Cytomegalovirus and herpesvirus research
- Neuroinflammation and Neurodegeneration Mechanisms
- Systemic Lupus Erythematosus Research
- Alcohol Consumption and Health Effects
- Ocular Diseases and Behçet’s Syndrome
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Urticaria and Related Conditions
- Pain Mechanisms and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Retinal Diseases and Treatments
- Liver Disease Diagnosis and Treatment
- Retinal Development and Disorders
- Antimicrobial Peptides and Activities
- Herbal Medicine Research Studies
Jikei University School of Medicine
2014-2023
Tokyo Women's Medical University
1989-2010
Gunma University
1959-2010
Kitasato University
2010
Saitama Medical University
2010
Tokyo Medical University
2010
Niigata University
2010
Tokyo Metropolitan Institute of Medical Science
2010
University of Toyama
2010
Nagoya Women's University
2008
Abstract We investigated the effects of a single dose mouse interleukin‐31 ( IL ‐31) on scratching behaviour in comparison with spontaneous skin‐lesion‐ or serotonin (5‐ HT )‐ induced NC /Nga and BALB /c mice. Intradermal (i.d.) injection ‐31 caused gradual increase long‐lasting LLS , over 1.5 s) about 3 h after administration followed by decrease for 24 administration. I.d. significantly increased total counts/24 but not short‐lasting SLS 0.3–1.5 s). In skin‐lesioned mice, counts were...
Peptide immunotherapy using dominant T-cell epitopes is safer and more effective than conventional for the treatment of immunoglobulin E (IgE)-mediated allergic diseases. When allergenic epitope peptides are expressed in edible part transgenic plants, successful mucosal immune tolerance to these allergens may be attainable by consumption plants. In this study, we generated rice seed that accumulated high concentrations (about 60 microg per grain) polypeptide consisting seven human derived...
Toxic shock syndrome toxin-1 (TSST-1)-binding structures present on murine lymphoid tissues were investigated by using 125I-TSST-1. T-depleted C57BL/6 spleen cells incubated with TSST-1 for 3 h at 0 degree C mitogenic to splenic T cells, indicating that the former bind and cells. TSST-1-binding activity was observed in B L transfected I-Ab genes, but not control Scatchard plot analysis showed these transfectants bound similar binding affinity. SDS-PAGE lysates of I-Ab-positive contain a...
OBJECTIVE: Cytokine interleukin-1β plays a central role in the inflammation process. Serum levels of IL-1β are elevated patients with alcoholic liver disease (ALD), especially those cirrhosis and hepatitis. Recently, presence genetic polymorphisms this cytokine was confirmed. The aim study to determine whether associated development ALD. METHODS: We examined frequency two gene located promoter −511 exon 5 +3953 locus by restriction fragment length 142 male ALD, 30 heavy drinkers without 218...
Asthmatic patients have a higher susceptibility to rhinovirus (RV) infection, and impaired IFN-β IFN-λ production has been demonstrated in bronchial epithelial cells from asthmatic adults upon exposure RV. However, the mechanisms underlying increased of RV infection remain poorly understood. The present study aimed elucidate characteristics immune responses patients' peripheral blood mononuclear (PBMCs) exposure.PBMCs obtained 3 different age groups (2-6 years: young-children group; 7-19...
Though accumulating evidence suggests that microglia, resident macrophages in the retina, and bone marrow-derived can cause retinal inflammation which accelerates photoreceptor cell death, details of how these cells are activated during degeneration (RD) remain uncertain. Therefore, it is important to clarify play a dominant role fueling inflammation. However, distinguishing between microglia difficult using conventional techniques such as markers (e.g., Iba-1). Recently, two mouse models...
Atopic dermatitis (AD) is a common skin disease caused by genetic and environmental factors. However, the mechanisms underlying AD development remain unclear. In this study, we examined factors contributing to onset of itch-associated scratching in different strains mice. Interleukin-31 (IL-31) induces severe site action IL-31 remains Cutaneous receptor A (IL-31RA) mRNAs dorsal root ganglion (DRG) are expressed exclusively model, i.e., NC/Nga Here evaluated effects repeated administration on...
The tolerance and pharmacokinetics of fleroxacin were studied in healthy male adult volunteers. peak serum concentrations unchanged about 1·5, 3 5 mg/1 at 1–2 h after single oral doses 100, 200 400 mg, respectively. apparent elimination half-life was 10 h, independent the dose. Fleroxacin, demethyl TV-oxide excreted urine over days accounted for 75%, 5% 5%, respectively, doses. drug dose-related; mean concentrations, sustained 24 50, 100 150 mg doses, Food intake did not significantly...
Prokineticin 2 (PK2) expression is upregulated in mice with collagen-induced arthritis (CIA), an animal model of rheumatoid arthritis. The purpose our study was to investigate the effects PK2 inhibition on CIA. PK2, prokineticin receptor (PKR) 1, and PKR2 mRNA transcripts joints CIA were measured by real-time PCR Days 21, 28, 35 (n = 15/day). Localization PKR1 proteins examined immunohistochemically. PKRA7, a antagonist, administered intraperitoneally for weeks mice, severity compared...
We evaluated the clinical significance of telomerase activity and telomere length peripheral blood mononuclearcells (PBMC) in systemic lupus erythematosus (SLE). PBMC were isolated from 55 patients with SLE was measured by TRAP assay. The also 30 these subjects. As a control group, 45 healthy adults no particular history studied. results compared data. In active SLE, significantly increased group. inactive not different controls their 20s, 30s 40s, but it 50s. patients, correlatedwith...
Summary To develop a new immunotherapy for Japanese cedar ( Cryptomeria japonica; CJ) pollinosis, we evaluated the use of DNA immunization by inoculating mice with plasmid encoding Cry j 1 as CJ pollen major allergen (pCACJ1). Repeated intramuscular (i.m.) inoculation BALB/c pCACJ1 produced anti‐Cry antibody responses, which were predominately immunoglobulin G2a (IgG2a) type. Furthermore, this suppressed E (IgE) and IgG1 responses to subsequent alum‐precipitated injections. Splenic T cells...
Pineal parenchymal tumor (PPT) cells usually show immunoreactivity for synaptophysin, neuron-specific enolase, neurofilament protein, class III β-tubulin, tau PGP9.5, chromogranin, serotonin, retinal S-antigen, and rhodopsin, but these markers are not specific PPTs. Melatonin is produced secreted mainly bypineal cells; hydroxyindole-O-methyltransferase (HIOMT) catalyzes the final reaction in melatonin biosynthesis. We hypothesized that HIOMT could serve as a marker of PPTs, we investigated...
<i>Background:</i> Some chemical compounds in the environment worsen allergic inflammation. In this study, we examined whether organic solvents induce production of thymic stromal lymphopoietin (TSLP) which elicits Th2-type immune responses. <i>Methods:</i> Organic were painted on earlobes BALB/c mice. The expression TSLP ear was determined by ELISA. <i>Results:</i> Xylene and toluene, but not chloroform or ethyl acetate, induced mRNA for earlobe tissue....
Retinal inflammation accelerates photoreceptor cell death caused by retinal degeneration. Minocycline, a semisynthetic broad-spectrum tetracycline antibiotic, has been previously reported to rescue in We examined the effect of minocycline on degeneration using c-mer proto-oncogene tyrosine kinase ( Mertk) −/− Cx3cr1 GFP/+ Ccr2 RFP/+ mice, which enabled observation CX3CR1-green fluorescent protein (GFP)- and CCR2-red (RFP)-positive macrophages fluorescence. Retinas Mertk mice showed...