NS-398 (N-(2-cyclohexyloxy-4-nitrophenyl) methane sulphonamide), a newly synthesized potent non-steroidal anti-inflammatory drug (NSAID) has much lesser degree of toxicity, as compared with presently available NSAIDs. We have investigated the inhibition prostanoid production in inflammatory exudate, gastric mucosa and renal papillary tissue, following oral administration to carrageenan-air-pouch rats. The ID50 values tissue were 0.18, 62.2 261.7 mg kg-1, respectively. In contrast,...

Abstract: Pruritus is an important symptom in atopic dermatitis (AD), but the major pruritogen have not been identified. NC/Nga mice, spontaneously develop eczematous AD‐like skin lesion when kept under conventional conditions, specific pathogen‐free (SPF) thought to be animal model for AD. In this study, determine whether newly identified cytokine, IL‐31, may involved pruritus of AD, we examined IL‐31 expression spontaneous which showed itch‐associated long‐lasting (over 1.5 s duration)...

Abstract We investigated the effects of a single dose mouse interleukin‐31 ( IL ‐31) on scratching behaviour in comparison with spontaneous skin‐lesion‐ or serotonin (5‐ HT )‐ induced NC /Nga and BALB /c mice. Intradermal (i.d.) injection ‐31 caused gradual increase long‐lasting LLS , over 1.5 s) about 3 h after administration followed by decrease for 24 administration. I.d. significantly increased total counts/24 but not short‐lasting SLS 0.3–1.5 s). In skin‐lesioned mice, counts were...

Abstract Background RNA interference (RNAi) is rapidly becoming a major tool that revolutionizing research in the bioscience and biomedical fields. To apply RNAi technique vivo , it crucial to develop appropriate methods of guiding short interfering (siRNA) molecules right tissues cells. Here, we demonstrate an efficient method for performing gene knockdown body skin using electro‐transduction siRNA. Using this method, examined whether targeted silencing cyclooxygenase (COX) could modulate...

Atopic dermatitis (AD) is a common skin disease caused by genetic and environmental factors. However, the mechanisms underlying AD development remain unclear. In this study, we examined factors contributing to onset of itch-associated scratching in different strains mice. Interleukin-31 (IL-31) induces severe site action IL-31 remains Cutaneous receptor A (IL-31RA) mRNAs dorsal root ganglion (DRG) are expressed exclusively model, i.e., NC/Nga Here evaluated effects repeated administration on...

When mice were given an i.v. injection of LPS 7 days after Propionibacterium acnes, liver injury and a rapid increase serum alanine aminotransferase asparagine acid occurred. The in vivo administration mAb against LFA-1 on 1, 2, 3 the P. acnes resulted potent inhibition all these dysfunctions. Using model, we found that anti-LFA-1 protected from LPS-induced lethal shock. During course injury, inflammatory cells infiltrated caused massive hepatic cell necrosis. Flow cytometry revealed...

Itching is a characteristic symptom in various forms of dermatosis, especially atopic dermatitis; consequently it major diagnostic criterion. All features are similar to events seen patients, hence NC/Nga mice considered be suitable model human dermatitis. However, there were data spreads commencing time and the degree skin lesions mice.In present study, we attempted improve experimental conditions induce stable establish more appropriate method. Methods kept together with skin-lesioned...

Atopic dermatitis (AD) is related to immunoglobulin E (IgE) production, and a type-1 type-2 helper T cell (Th1/Th2) imbalance has been hypothesized as the aetiology. While itching scratching are important factors in development of dermatitis, mechanisms underlying these phenomena poorly understood. We investigated relationship between scratching, transepidermal water loss (TEWL), signs serum Ig levels NC/Nga mice, model AD. also sensitized specific pathogen-free (SPF)-NC/Nga mice BALB/c mite...

NC/Nga (NC) mice with similar pathological and behavioural features as seen in human atopic dermatitis are used a model of the disease. Under normal circumstances, spontaneous persistent scratching occurs NC this can lead to onset skin inflammation.We examined induction behaviour NC, BALB/c, ICR C3H/HeN mice, BALB/c by cohabitation dermatitis.NC, were kept together chronic (CNV-NC) for 2 weeks, numbers episodes counted. also CNV-NC 24 weeks severity score was assessed. The assessed further 8...

Abstract: NC/Nga mice have pathological and behavioral features similar to those seen in human atopic dermatitis. There are two known dermatitis models mice, one being spontaneous‐induced under conventional conditions the other 2,4,6‐trinitrochlorobenzene (TNCB)‐induced allergic contact However, there significant differences time course on development of We studied role scratching behavior (sign itch) spontaneous‐ TNCB‐induced measured counts, transepidermal water loss (TEWL), skin...

Abstract: NC/Nga mice are known to develop scratching dermatitis akin atopic dermatitis, under conventional (Conv), but not the specific‐pathogen‐free (SPF) condition. In this study, we examined effects of mechanical‐scratching on spontaneous counts (sign itching), in relation cutaneous prostaglandin D 2 (PGD ) levels or BALB/c mice. Mechanical‐scratching increased barrier damage and PGD both strain SPF By 4 weeks cohabitation with skin‐lesioned mice, increase development score were higher...

Interleukin-31 (IL-31) is a recently identified cytokine produced by Th2 cells that involved in the development of atopic dermatitis-induced skin inflammation and pruritus. Its receptor, IL-31RA, expressed number cell types, including epithelial cells, eosinophils, activated monocytes macrophages. To date, however, regulation responses distinct types tissues expressing IL-31RA has not been well studied. In this study, Cry j 2, one major allergens Japanese cedar pollen, was administered to...