A5058993790- Plant Genetic and Mutation Studies
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- GABA and Rice Research
- DNA and Nucleic Acid Chemistry
- Radiation Effects and Dosimetry
- Metal complexes synthesis and properties
- Agronomic Practices and Intercropping Systems
- Plant Reproductive Biology
- Seed and Plant Biochemistry
- Synthesis and Biological Evaluation
- Silicon Effects in Agriculture
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- Rabbits: Nutrition, Reproduction, Health
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Electron Spin Resonance Studies
- Rice Cultivation and Yield Improvement
- Light effects on plants
- Plant Growth Enhancement Techniques
- Maternal and fetal healthcare
- Plasma Applications and Diagnostics
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
Pondicherry University
2008-2020
Oklahoma State University
2008-2009
Oakland University
2004-2005
University of Rochester
2004
Jawaharlal Nehru University
2001
Bhabha Atomic Research Centre
2001
MicroRNAs (miRNAs) and small-interfering RNAs (siRNAs) have emerged as important regulators of gene expression in higher eukaryotes. Recent studies indicate that genomes plants encode lineage-specific species-specific miRNAs addition to the well-conserved miRNAs. Leguminous are grown throughout world for food forage production. To date lack genomic sequence data has prevented systematic examination small leguminous plants. Medicago truncatula, a diploid plant with near-completely sequenced...
Purpose: The profitable production of some important plant-based secondary metabolites (ginsenosides, saponins, camptothecin, shikonins etc.) in vitro by gamma irradiation is a current area interest. We reviewed different types metabolites, their mode synthesis and effect γ-radiation on yield for plants, organs cultures (callus, suspension, hairy root). Special effort has been made to review the biochemical mechanisms underlying increase metabolites. A comparison improvement with biotic...
Electron spin resonance (ESR) studies of radicals formed by radiation-induced multiple one-electron oxidations guanine moieties in DNA are reported this work. Annealing gamma-irradiated from 77 to 235 K results the hydration one electron oxidized (G •+ ) form 8-hydroxy-7,8-dihydroguanin-7-yl-radical (•GOH) having β-proton coupling 17–28 G and an anisotropic nitrogen coupling, A‖ , ∼20 G, A⊥ = 0 with g‖ 2.0026 g⊥ 2.0037. Further annealing 258 formation a sharp singlet at g 2.0048 line-width...
Electron spin resonance (ESR) studies of radicals formed by radiation-induced multiple one-electron oxidations guanine moieties in DNA are reported this work. Annealing gamma-irradiated from 77 to 235 K results the hydration one electron oxidized (G*+) form 8-hydroxy-7,8-dihydroguanin-7-yl-radical (*GOH) having beta-proton coupling 17-28 G and an anisotropic nitrogen coupling, A(parallel), approximately 20 G, A(perpendicular) = 0 with g(parallel) 2.0026 g(perpendicular) 2.0037. Further...
Shukla, L. I., Pazdro, R., Huang, J., DeVreugd, C., Becker, D. and Sevilla, M. The Formation of DNA Sugar Radicals from Photoexcitation Guanine Cation Radicals. Radiat. Res. 161, 582–590 (2004).In this investigation radical formation reaction in γ- irradiated model compounds, we report the conversion guanine cation (one-electron oxidized guanine, G· ) to C1′ sugar another at C3′ or C4′ position (designated C3′·/C4′·) by visible UV photolysis. Electron spin resonance (ESR) spectroscopic...
In this investigation of the radical formation and reaction radicals in gamma-irradiated DNA, we report isolation putative neutral by scavenging holes Fe(CN)6(4-) electrons Fe(CN)6(3-). Experiments are performed under conditions that emphasize direct quasi-direct effects (collectively called direct-type effects.) Samples containing show effective ESR spectra obtained arise principally from DNA anion radicals. On other hand, for samples Fe(CN)6(3-), electron is highly efficient, resulting...
miRNA are the post transcriptional regulator of genes. The conserved miR168 family is evaluated for position based nucleotide preference in higher plants. Low density lipoprotein receptor adaptor protein 1 (LDLRAP1) target validated miR168a obtained from rice origin reported.The mature sequences include miR168-5p and miR168-3p, were miRBase (v21, June 2014) 15 families (28 plants). preferred using Data Analysis Molecular Biology Evolution software. was subjected to cross kingdom analysis...
The miR398 (microRNA398) posttranscriptionally regulates the superoxide dismutase (Cu/Zn-SOD).The level was quantitated in gamma-irradiated (20Gy and 200Gy) nitrogen-stressed seedlings by stem-loop RT-PCR (Real Time-Polymerase chain reaction). positional preference of nucleotide (nt) for families new targets done. SOD enzyme assayed native PAGE (Polyacrylamide gel electrophoresis).A relative increase miR398-3p expression roots, reduction shoots tissue downregulation miR398-5p is noted....
Electron paramagnetic resonance (EPR) studies were conducted on barley seeds exposed to normal (H2O) and deuterated (D2O) moisture, irradiated with 750 Gy at 77 K. Reported here, for the first time, are trapped electrons formed γ-irradiation of Electrons stabilized/solvated an increase in moisture content (H2O/D2O) seeds. The recombination electron radical cation gave intense thermoluminescence emission 110 With temperature destruction singlet, unmasking underlying heterogeneous population...
Low-temperature plasma (LTP) processing is a green and flexible food technology with only known limitation of low energy efficiency. Three established fundamental mechanisms includes: i) physical damage to membranes internal cellular components due bombardment energetic ions: ii) radicals charged species led chemical reactions: iii) radiation-induced modifications. In this investigation, we present new mechanism generation extraction $N_2$ neutrals from glow discharge plasmas for efficient...