A5037221451- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Infections and bacterial resistance
- Plant Disease Resistance and Genetics
- Force Microscopy Techniques and Applications
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Fungal Diseases
- Mycobacterium research and diagnosis
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Plant and Fungal Interactions Research
- Antimicrobial Resistance in Staphylococcus
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Gene expression and cancer classification
- Bacterial biofilms and quorum sensing
- RNA Interference and Gene Delivery
- Insect-Plant Interactions and Control
- Bacterial Identification and Susceptibility Testing
- Epigenetics and DNA Methylation
- Trace Elements in Health
- RNA Research and Splicing
- Plant Pathogens and Resistance
- RNA regulation and disease
Oklahoma State University
2007-2022
Oklahoma State University Oklahoma City
2019
Agricultural Research Service
2014
Pennsylvania State University
2014
New Mexico State University
2013
Oak Ridge National Laboratory
1990-2004
University of Tennessee at Knoxville
1993-2004
California State University, Fresno
1997
Arizona State University
1997
European Space Research and Technology Centre
1997
MicroRNAs (miRNAs) and small-interfering RNAs (siRNAs) have emerged as important regulators of gene expression in higher eukaryotes. Recent studies indicate that genomes plants encode lineage-specific species-specific miRNAs addition to the well-conserved miRNAs. Leguminous are grown throughout world for food forage production. To date lack genomic sequence data has prevented systematic examination small leguminous plants. Medicago truncatula, a diploid plant with near-completely sequenced...
The Russian wheat aphid, Diuraphis noxia Kurdjumov, is one of the most important pests small grains throughout temperate regions world. This phytotoxic aphid causes severe systemic damage symptoms in wheat, barley, and other as a direct result salivary proteins it injects into plant while feeding. We sequenced de novo assembled genome D. Biotype 2, strain virulent to resistance genes wheat. genomic scaffolds span 393 MB, equivalent 93% its 421 MB genome, contains 19,097 genes. has AT-rich...
DNase I footprinting assays were performed to identify the binding sites for putative trans-acting factors involved in control of alpha-fetoprotein (AFP) gene expression using mouse AFP promoter fragments (-839 +56) and nuclear protein extracts from fetal, newborn, adult livers brain kidney. Our studies have shown that with liver, there are 14 protected regions up -839 base pairs (bp). Region (-82 -43) was by at least three different factors, one which is CCAAT-binding/enhancer-binding...
RNA interference (RNAi) has become a powerful biological tool over the past decade. In this study, tetracycline-inducible small hairpin (shRNA) vector system was designed for silencing cyan fluorescent protein (CFP) expression and delivered alongside yfp marker gene into Chinese hamster ovary cells using impalefection on spatially indexed vertically aligned carbon nanofiber arrays (VACNFs). The VACNF architecture provided simultaneous delivery of multiple genes, subsequent adherence...
Early stage infections caused by fungal/oomycete spores may not be detected until signs or symptoms develop. Serological and molecular techniques are currently used for detecting these pathogens. Next-generation sequencing (NGS) has potential as a diagnostic tool, due to the capacity target multiple unique signature loci of pathogens in an infected plant metagenome. NGS significant diagnosis important eukaryotic However, assembly analysis huge amounts sequence is laborious, time consuming,...
BoHV-1, a neurotropic herpesvirus, establishes, maintains, and reactivates from latency in neurons. BoHV-1 DNA is also detected pharyngeal tonsil (PT) latently infected calves.
Vibrio (Beneckea) harveyi , a bioluminescent marine bacterium, has been shown to produce bacteriocin-like substance the production of which is mediated by plasmid. This assumed be proteinaceous because its sensitivity certain proteolytic enzymes. It stable at low temperatures and can concentrated ammonium sulfate precipitation or negative-pressure dialysis. The molecular weight bacteriocin was determined 2.4 × 10 4 exclusion chromatography. Competition experiments indicated that...
The advancement in high-throughput sequencing (HTS) technology allows the detection of pathogens without need for isolation or template amplification. Plant regulatory agencies worldwide are adopting HTS as a prescreening tool plant imported germplasm. technique is multipronged process and, often, bioinformatic analysis complicates detection. Previously, we developed E-probe diagnostic nucleic acid (EDNA), that detects data. EDNA uses custom databases signature sequences (e-probes) to reduce...
Next generation sequencing (NGS) is not used commonly in diagnostics, part due to the large amount of time and computational power needed identify taxonomic origin each sequence a NGS data set. By using unassembled sets as target for searches, pathogen-specific sequences, termed e-probes, could be queries enable detection specific viruses or organisms plant sample metagenomes. This method, designated e-probe diagnostic nucleic acid assay, first tested with mock databases, was generated from...
The Elizabethkingia are a genetically diverse genus of emerging pathogens that exhibit multidrug resistance to range common antibiotics. Two representative species, bruuniana and meningoseptica, were phenotypically tested determine minimum inhibitory concentrations for five Ultra-long read sequencing with Oxford Nanopore Technologies subsequent de novo assembly produced complete, gapless circular genomes each strain. Alignment based annotation Prokka identified 5,480 features in E. 5,203...
Abstract The Alpine goat ( Capra aegagrus hircus ) is parasitized by the barber pole worm Haemonchus contortus ). Hematological parameters from transcript and metagenome analysis in host are reflective of infestation. We explored comparisons between blood samples control, infected, infected zoledronic acid-treated, antibody (anti-γδ T cells) treated wethers under controlled conditions. Seven days post-inoculation (dpi), we identified 7,627 transcripts associated with different treatment...
Elizabethkingia meningoseptica is ubiquitous in nature, exhibits a multiple-antibiotic resistance phenotype, and causes rare opportunistic infections. We now report two draft genome sequences of E. type strains that were sequenced independently laboratories.
E-probe Diagnostic for Nucleic acid Analysis (EDNA) is a bioinformatic tool originally developed to detect plant pathogens in metagenomic databases. However, enhancements made EDNA increased its capacity conduct hypothesis directed detection of specific gene targets present transcriptomic To target pathogenicity factors used by the pathogen infect host or other interest, e-probes need be transcripts related that function. In this study, transcriptomics (EDNAtran) was expression genes...
Immature brown shrimp were marked with plastic streamer tags, then observed in large tanks for the effect of tags on mortality under a variety pre- and post-tagging treatment regimes. Except where tagged animals held at low density (9 animals/m2) few untagged conspecifics (2/m2), was about 50% higher among than controls. Differences groups number handling steps, wound caused by tagging needle, duration high-density holding prior to release, sex test independent mortality. The identity tagger...
Brain zinc dysregulation is linked to many neurological disorders. However, the mechanisms regulating brain homeostasis are poorly understood. We performed secondary analyses of MRI GWAS and exome sequencing data from adults in UK Biobank. Coding ZIP12 polymorphisms transporter (SLC39A12) were associated with altered susceptibility weighted (swMRI). Conditional joint association revealed independent signals linkage disequilibrium 2 missense polymorphisms, rs10764176 rs72778328, reduced...
We report the draft genome of Elizabethkingia strain ATCC 33958, which has been classified as miricola. Similar to other species, 33958 contains numerous β-lactamase genes. also harbors a urease gene cluster supports classification E.
This paper reports an exploratory study based on quantitative genomic analysis in dairy traits of American Alpine goats. The are quality-determining components goat milk, cheese, ice cream, etc. phenotypes for quality have been routinely recorded many years and deposited the Council Dairy Cattle Breeding (CDCB) repository. data collected were used to conduct genome-wide association (GWAS) from 72 female goats originating locations throughout U.S. Genotypes identified with Illumina Goat 50K...