Regulation of intracellular cyclic adenosine 3 ',5 '-monophosphate (cAMP) is integral in mediating cell growth, differentiation, and immune responses hematopoietic cells. To facilitate studies cAMP regulation we developed a BRET (bioluminescence resonance energy transfer) sensor for cAMP, CAMYEL (cAMP using YFP-Epac-RLuc), which can quantitatively rapidly monitor concentrations vivo. This was used to characterize three distinct pathways modulation synthesis stimulated by presumed...

Melasma is a pigmentary disorder characterized by hyperpigmented patchy skin in sun-exposed areas, especially the face. Treatment of melasma can be challenging because long-term therapy required, reoccurrence common, and existing therapies are insufficient unsatisfactory. To investigate new treatment options, we performed an exploratory double-blinded, randomized split-face study to assess efficacy tyrosinase inhibitor Thiamidol compared hydroquinone women with mild moderate melasma. After...

The second messenger cAMP plays a critical role in regulating immune responses. Although well known for its immunosuppressive effect, is also required the development of optimal Thus, regulation this needs to be finely tuned and balanced context dependent manner. To further understand synthesis functions system, we focus on specific adenylyl cyclase (AC) isoform, AC VII (AC7), which highly expressed system. We show that mice deficient AC7 are hypersensitive LPS-induced endotoxic shock....

ABSTRACT We investigate how temporal and spatial interactions between multiple intercellular intracellular factors specify the fate of a single cell in Caenorhabditis elegans. P12, which is ventral cord neuroectoblast, divides postembryonically to generate neurons unique epidermal cell. Three classes proteins are involved specification P12 fate: LIN-3/LET-23 growth factor signaling pathway, Wnt protein LIN-44 its candidate receptor LIN-17, homeotic gene product EGL-5. show that LIN-3 an...

Regulation of intracellular cAMP by multiple pathways enables differential function this ubiquitous second messenger in a context-dependent manner. Modulation G(s)-stimulated has long been known to be modulated the G(i) and G(q)/Ca(2+) pathways. Recently, G(13) pathway was also shown facilitate responses murine macrophage cells. We report here that synergistic regulation synthesis G(s) is mediated specific isoform adenylyl cyclase, AC7. Furthermore, signaling paradigm exists several...

Sun-protective behavior affects skin cancer prevention. Shade works by physically shielding from direct harmful UV rays; however, may still remain exposed to reflected and indirect rays. There is no current standard metric evaluate shade for its effectiveness in sun protection, there insufficient clinical evidence that a beach umbrella alone can provide adequate protection.To directly measure sunburn protection offered compared with provided sunscreen high factor under actual use...

Despite being exposed to multiple ligands, a macrophage cell line exhibits surprisingly limited number of responses.

Growth factor preparations have demonstrated effectiveness in reversing age-related changes facial skin. TNS® Advanced+ Serum (TNS A+ Serum; SkinMedica®, Allergan Aesthetics, an AbbVie Company) and TNS Pro-Infusion for DiamondGlow® (DG-TNS A+; Aesthetics) combine growth technology with active botanical ingredients to target signs of skin aging.

Background Facial wrinkles are an undesirable feature caused by extrinsic photodamage and intrinsic aging process. Many cosmetic products esthetic procedures strive to ameliorate the appearance of wrinkles. Currently effects those on mainly evaluated clinical grading, subjective self‐assessment questionnaires, optical profilometry replica impressions. An objective quantitative method is in demand. Methods Raking light was applied directly facial photography cast as dark shadows. The...

The aging process involves numerous biological mechanisms that have been characterized and proposed as the "hallmarks of aging." Targeting processes pathways related to these hallmarks cause promote skin could provide anti-aging benefits. A novel topical growth factor-based care serum (A+) was developed using human fibroblast conditioned media. This study aimed assess effects A+ on four its clinical efficacy in rejuvenation subjects with moderate severe overall facial photodamage.Preclinical...

As a result of their complex aetiology and periodicity, dark circles are difficult to characterize measure, with current assessment techniques relying on specialist equipment, image analysis or proprietary grading scales. There is therefore need develop validate photonumeric scale for assessing infraorbital circles, which can provide an objective consumer relevant tool evaluating this condition the efficacy treatment products procedures.

To examine the effect of sodium copper chlorophyllin complex on expression biomarkers photoaged dermal extracellular matrix indicative skin repair.Following a previously published 12-day clinical assessment model, biopsy samples from forearms four healthy females with signs were obtained and analyzed by immunohistochemistry for key aging after each subject was treated test material consisting gel containing liposomal dispersion 0.05%, positive control tretinoin cream 0.025%, an untreated...

Purpose: There is growing interest in combining topical treatments with aesthetic procedures to combat signs of aging skin. This study aimed assess the efficacy and tolerability a novel cosmetic serum containing 5 different forms HA (HA DG) when used via proprietary diamond-tip microdermabrasion procedure (DG) treat skin dryness, fine lines/wrinkles, rough texture, dullness. Patients Methods: In this open-label, single-center study, participants received DG as part biweekly on face neck for...

Summary Introduction An antiaging regimen that aids in clearing the matrix of waste products and stimulating neocollagenesis neoelastogenesis was tested among a group subjects over course 12 weeks to assess its efficacy women with mild moderate wrinkles skin sagging on face. Materials methods The product 22 using investigator clinical grading measurements, raking light imaging, 3D biopsies, self‐assessment questionnaires at baseline 4, 8, 12. Results Clinical indicated use for produced...

Regulation of multiple adenylyl cyclases (AC) provides unique inputs to mediate the synthesis cAMP, a ubiquitous second messenger that controls many aspects cellular function. On stimulation by G_s, activities ACs can be further selectively modulated other pathways ensure precise control intracellular cAMP responses specific stimuli. Recently, we reported one AC isoforms, AC7, is uniquely regulated G₁₃ pathway. To understand more fully molecular mechanism this...

Abstract Background Aging of upper arm skin, induced by intrinsic and extrinsic factors, often results in a loss contour, elasticity, firmness, an increase laxity, crepiness, roughness, photodamage. A topical body firming moisturizer (TBFM) was developed to target all aspects skin aging. Objectives The aim this study evaluate the efficacy tolerability TBFM for rejuvenation. Methods Forty female subjects, 40 60 years old, Fitzpatrick type II V, with mild moderate photodamage on arms, were...

Photoaged skin results from various environmental factors, most importantly chronic sun exposure. Dyschromia and fine lines/wrinkles are common clinical manifestations of photodamaged skin.This single-center trial was conducted to assess the efficacy tolerability a new multifunctional facial primer (camouflage, broad-spectrum SPF 50, treatment for hyperpigmentation) when used by females with mild-to-moderate hyperpigmentation lines due photoaging over course 12 weeks.Subjects were provided...