Abstract The in vitro antifungal activity of chitosan against Fusarium oxysporum f. sp. cubense Race 4 (FocR4) the causal agent banana wilt was investigated. Chitosan at all concentrations tested reduced hyphal growth FocR4 on potato dextrose agar media and recording maximum inhibition 76.36% 8 mg/mL. inhibitory effect found to increase as concentration increases. 50% effective value estimated by probit analysis, it 1.4 more broth where completely inhibited mycelial tested. sporulation a...

A Streptomyces isolate having antifungal activity against Pyricularia oryzae, the causal agent of rice blast disease, was isolated from soil collected in fields Tanjung Karang Selangor, peninsula Malaysia.The aim study to determine sp. UPMRS4 extracts P. oryzae and identify bioactive compounds produced by UPMRS4. Various solvents were used for extraction well diffusion method extracts. The ethyl acetate extract demonstrated highest mycelial growth with an effective inhibitory concentration...

Basal stem rot (BSR), caused by the basidiomycete fungus, Ganoderma boninense, is an economically devastating disease in Malaysia. Our study investigated changes lignin content and composition along with activity expression of phenylpropanoid pathway enzymes genes oil palm root tissues during G. boninense infection. We sampled control (non-inoculated) infected (inoculated) seedlings at seven time points (1, 2, 3, 4, 8, 12 weeks post-inoculation (wpi)) a randomized design. The profiles...

Purpose: IIn Malaysia, jackfruit (variety Tekam Yellow) significantly contributes to the nation’s economy. The valuable fruit production is affected by bronzing disease (JBD). Information on JBD occurrence in Peninsular Malaysia necessary develop effective control strategies. Hence, this study aimed survey and identify corresponding causative pathogens selected orchards distributed Malaysia.Research Method: A total of eight from five states were for sampling. Fruit samples described...

Ganoderma boninense ( G. ) has been identified as a major problem in oil palm industry which caused basal stem rot disease. Identification of metabolite variation healthy and -infected leaves at 14 days postinfection using NMR metabolomics approach followed by characterization an electrochemical sensor based on functionalized multiwalled carbon nanotube (MWCNT) layer-by-layer framework modified screen-printed electrode successfully determined. Significant differences from the 1 H data were...

A highly efficient and reproducible Agrobacterium-mediated transformation protocol for Ganoderma boninense was developed to facilitate observation of the early stage infection basal stem rot (BSR). The method proven amenable different explants (basidiospore, protoplast, mycelium) G. boninense. efficiency highest (62%) under a treatment combination protoplast explant Agrobacterium strain LBA4404, with successful expression an hyg marker gene gus-gfp fusion control heterologous p416...

Latex production from Hevea brasiliensis rubber tree is the second most important commodity in Malaysia, but this industry threatened by white root rot disease (WRD) caused Rigidoporus microporus that leads to considerable latex yield loss and death. This study aimed characterize compare virulence of five R. isolates obtained infected trees located at different states Malaysia. These were subjected morphological molecular characterization for species confirmation pathogenicity test...

Rigidoporus microporus, which causes white root rot disease (WRD) in Hevea brasiliensis, is a looming threat to rubber plantation Malaysia. The current study was conducted determine and evaluate the efficiency of fungal antagonists (Ascomycota) against R. microporus trees under laboratory nursery conditions. A total 35 isolates established from tree rhizosphere soil were assessed for their antagonism by dual culture technique. Trichoderma can inhibit radial growth 75% or more test. Strains...

Hevea brasiliensis is a natural source of rubber and an important plantation tree species in Malaysia. Leaf blight disease caused by Fusicoccum substantially reduces the growth performance H. brasiliensis. The aim this study was to use combination both morphological characteristics molecular data clarify taxonomic position fungus associated with leaf disease. were isolated from infected leaves collected plantations at 3 widely separated locations - Selangor, Perak, Johor states Peninsular...

Soybean (Glycine max L.) is one of the most economically important crops in world, and anthracnose known to infect soybean countries. Colletotrichum truncatum common pathogen causing soybean. However, at least five species have been reported on worldwide (2). In July 2010, symptoms were observed experimental fields agriculture station Ladang Dua, University Putra Malaysia located Selangor state Malaysia. Symptoms initially a few plants randomly within field, but after 4 weeks, disease was...

Abstract Pseudomonas aeruginosa developed its biocontrol agent property through the production of antifungal derivatives, with phenazine among them. In this study, applications crude synthesized by UPMP3 and hexaconazole were comparatively evaluated for their effectiveness to suppress basal stem rot infection in artificially G. boninense -challenged oil palm seedlings. A glasshouse experiment under randomized completely block design was set following treatments: non-inoculated seedlings,...

Basal stem rot (BSR) of oil palm is a disastrous disease caused by white-rot fungus Ganoderma boninense Pat. Non-ribosomal peptides (NRPs) synthesized non-ribosomal peptide synthetases (NRPSs) are group secondary metabolites that act as fungal virulent factors during pathogenesis in the host. In this study, we aimed to isolate NRPS gene G. strain UPMGB001 and investigate role boninense-oil interaction. The isolated DNA fragment 8322 bp was used predict putative sequence different domains...

Abstract P seudomonas aeruginosa strain UPMP 3 labelled with β ‐glucuronidase ( gusA ) and green fluorescent protein gfp by electrotransformation yielded ca 1 × 10 7 transformants µg −1 DNA . The data obtained from the dilution plate count showed that over 28 days both epiphytic endophytic populations of increased 5.76 log [colony forming unit CFU + 1] g fresh weight FW to 8.19 1) 4.10 6.23 , respectively. Confocal laser scanning microscopic analysis oil palm roots treated :gfp ‐tagged...