A5048280661- Orthopedic Surgery and Rehabilitation
- Tendon Structure and Treatment
- Reconstructive Surgery and Microvascular Techniques
- Dermatologic Treatments and Research
- Dupuytren's Contracture and Treatments
- Wound Healing and Treatments
- Surgical Sutures and Adhesives
- Nail Diseases and Treatments
- Shoulder Injury and Treatment
- Peripheral Nerve Disorders
- Cellular Mechanics and Interactions
- Facial Rejuvenation and Surgery Techniques
- Genital Health and Disease
- Bone fractures and treatments
- Body Contouring and Surgery
- Congenital limb and hand anomalies
- Reconstructive Facial Surgery Techniques
- Diagnosis and Treatment of Venous Diseases
- Angiogenesis and VEGF in Cancer
- Nerve Injury and Rehabilitation
- Chemotherapy-related skin toxicity
- Elbow and Forearm Trauma Treatment
- Cell Adhesion Molecules Research
- Nerve injury and regeneration
- Skin Protection and Aging
Singapore General Hospital
2017-2024
Duke-NUS Medical School
2017-2024
SingHealth Duke-NUS Academic Medical Centre
2024
Biomechanics Institute of Valencia
2023
Townsville Hospital
2020
University of Manchester
2007-2018
Wythenshawe Hospital
2003-2014
Erasmus MC
2013
Queen Elizabeth Hospital
2013
Erasmus University Rotterdam
2013
Many soft connective tissues are under endogenous tension, and their resident cells generate considerable contractile forces on the extracellular matrix. The present work was aimed to determine quantitatively how fibroblasts, grown within three-dimensional collagen lattices, respond mechanically precisely defined tensional loads. Forces generated in response changes applied load were measured using a culture force monitor. In number of variant systems, consistently reacted modify matrix...
The contraction of a collagen lattice by resident fibroblasts causes strains to be developed within that lattice. These can increased or decreased altering the aspect ratio (ratio length/width/thickness) fibroblast populated lattice, as cross-sectional area resisting strain is changed and application an external load. align themselves with direction maximum principle strain; in effect, these cells are "hiding" from perceived strain. pre-determined use computational finite element analysis....
Dupuytren's Disease (DD) is a common, fibroproliferative disorder affecting the palmar surface of hands which often irreversible and progressive. Understanding epidemiology DD important in order to provide clues its etiopathogenesis. This review aims evaluate epidemiological studies carried out since 1951. Studies evaluating were searched using Medline, Pubmed, Scopus dated back from 1951 current date. Inclusion criteria any investigating prevalence or incidence population group. A total 620...
Overproduction of collagen and its abnormal assembly are hallmarks keloid scars. Type I/III ratios altered in keloids compared with normal skin. Fibroblasts from different sites tissue, perilesional intralesional extralesional sites, show differential apoptosis contraction. Additionally, early vs. later cell culture passages display expression. We therefore hypothesize that fibroblasts the growing margin express higher levels type I III, production is by extended passage.(i) To measure III...
Background: There is a lack of rigorously validated patient-based outcomes measures scarring. The aim this study was to construct such scale and demonstrate reliability validity by applying the in wide range scarring samples. Methods: Patient Scar Assessment Questionnaire with five subscales (i.e., Appearance, Symptoms, Consciousness, Satisfaction Symptoms) constructed using multiple categorical response items. applied various surgical samples (total scar assessments n = 667) at months 3, 6,...
Background: The association between scarring and the depth of dermal injury or burn is clinically recognized but not quantified. authors tested hypothesis that there a critical beyond which fibrous scar develops. Methods: A novel jig produced wound was deep at one end superficial other. Pilot studies in cadaveric ex vivo breast skin confirmed injury. Healthy volunteers had standardized made on lateral aspect hip. Digital photography recorded surface appearance healing development....
Fibroblast contraction in wound healing involves the interaction of several cell types, cytokines, and extracellular matrix molecules. We have previously developed fibroblast alignment models using precise uniaxial mechanical loads 3D culture contact guidance on fibronectin strands. Our aim here was to use place fibroblasts their potentially most sensitive configuration, i.e., perpendicular axis loading, present cells with conflicting cues. Gene expression at mRNA level recovered from...
Skin scarring is associated with psychosocial distress and has a negative effect on quality of life. The transforming growth factor (TGF)-β family cytokines plays key role in scarring. TGF-β3 improves scar appearance range mammalian species. This study was performed to assess the efficacy intradermal avotermin (TGF-β3) for improvement following revision surgery.Sixty patients (35 men 25 women; age, 19 78 years; 53 Caucasians; length, 5 21 cm) received (200 ng/100 μl/linear cm wound margin)...
The ability of tendons to glide smoothly during muscle contraction is impaired after injury by fibrous adhesions that form between the damaged tendon surface and surrounding tissues. To understand how we incubated excised in fibrin gels (to mimic homeostatic environment at site) assessed cell migration. We noticed cells exiting from only cut ends. Furthermore, treatment with trypsin resulted extravagation shaft tendons. Electron microscopy immunolocalisation studies showed are covered a...
The palmar fascial ligaments have been examined by microdissection using an operating microscope in fresh and preserved cadaveric hands. fascia is seen to be a precise three dimensional system of skin having discreet transverse, longitudinal vertical fibre systems. In the normal hand there relative motion between ligament systems on movement. fibres provide anchorage which operates irrespective position underlying joints acts particularly resist shearing forces gripping. distribution lesions...
Bone marrow-derived endothelial progenitor cells (EPCs) have been detected in the peripheral blood of patients following thermal injury. EPCs migrate to sites active neovascularization response mediators released after trauma, contributing wound healing. The aim was characterize levels and kinetics burned patients, then relate these key mobilizing factors, vascular growth factor (VEGF) chemokine (C-X-C motif) ligand 12 (CXCL 12), compare them with those healthy subjects.The study included 19...