A5102846544- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Viral gastroenteritis research and epidemiology
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Hepatitis B Virus Studies
- Plant Parasitism and Resistance
- Ubiquitin and proteasome pathways
- Bacteriophages and microbial interactions
- Selenium in Biological Systems
- DNA Repair Mechanisms
- Bone health and treatments
- Hepatitis C virus research
- Glutathione Transferases and Polymorphisms
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Immune Response and Inflammation
- HIV Research and Treatment
- Genetic factors in colorectal cancer
- Plant Pathogens and Resistance
- Biofuel production and bioconversion
- Animal Virus Infections Studies
- Bone Metabolism and Diseases
- Viral Infections and Immunology Research
Indiana University Bloomington
1998-2017
Academia Sinica
2014
China University of Science and Technology
2014
Institute of Cellular and Organismic Biology, Academia Sinica
2009-2010
University of Wisconsin–Madison
1991-1992
Norovirus (NoV) is a positive-sense single-stranded RNA virus that causes acute gastroenteritis and responsible for 200,000 deaths per year worldwide. No effective vaccine or treatment available. Recent studies have shown the nucleoside analogs favipiravir (T-705) 2'-C-methyl-cytidine (2CM-C) inhibit NoV replication in vitro animal models, but their precise mechanism of action unknown. We evaluated molecular interactions between triphosphates RNA-dependent polymerase (NoVpol), enzyme...
Several Pseudomonas solanacearum strains which produced no detectable extracellular polysaccharide (EPS) in planta had been reported to remain highly virulent when tested at high inoculum concentrations (P. Xu, M. Iwata, S. Leong, and L. Sequeira, J. Bacteriol. 172:3946-3951, 1990; P. 170:617-622, 1988). Two of these mutants, KD700 KD710, have now molecularly genetically mapped the EPSI gene cluster described by Denny Baek (Mol. Plant-Microbe Interact. 4:198-206, 1991). When a range was...
Abstract The identification of molecules that are down‐regulated in malignant phenotype is important for understanding tumor biology and their role suppression. We compared the expression profile four normal nasal mucosal (NNM) epithelia a series nasopharyngeal cancinoma (NPC) cell lines using cDNA microarray confirmed actual selected genes, found osteoprotegerin (OPG) to be ubiquitously deficient NPC cells. also OPG various cancer lines, including oral, cervical, ovarian, lung, breast,...
Bacterial cell surface components can be important determinants of virulence. At least three gene clusters for extracellular polysaccharide (EPS) biosynthesis have been previously identified in the plant pathogen Pseudomonas solanacearum. We found that one these clusters, named ops, is also required lipopolysaccharide (LPS) biosynthesis. Mutations any complementation unit this cluster decreased EPS production, prevented binding an LPS-specific phage, and altered mobility purified LPS sodium...
Cell-penetrating peptides (CPP) can translocate across the cell membrane and have been extensively studied for delivery of proteins, nucleic acids, therapeutics in mammalian cells. However, characterizations CPP plants only recently initiated. We showed that intact virion a recombinant capsid protein (CaP) from plant-infecting nonenveloped icosahedral RNA virus, Brome mosaic virus (BMV), penetrate membranes plant protoplasts but are trapped by extracellular matrix. Furthermore, 22-residue...
Toll-like Receptor 3 (TLR3) detects double-stranded (ds) RNAs to activate innate immune responses. While poly(I:C) is an excellent agonist for TLR3 in several cell lines and human peripheral blood mononuclear cells, viral dsRNAs tend be poor agonists, leading the hypothesis that additional factor(s) are likely required allow respond dsRNAs. signaling was examined a lung epithelial line by quantifying cytokine production embryonic kidney cells luciferase reporter levels. Recombinant 1b...
A functional cure of chronic hepatitis B requires eliminating the virus (HBV)-encoded surface antigen (HBsAg), which can suppress immune responses. STOPS are phosphorothioated single-stranded oligonucleotides containing novel chemistries that significantly reduce HBsAgs produced by HBV-infected liver cells. The molecule ALG-10000 functions inside cells to levels multiple HBV-encoded molecules. However, it does not bind HBV An affinity resin coupled with was found several proteins from...
ABSTRACT Ralstonia solanacearum is the causal agent of bacterial wilt many agriculturally important crops. Exopolysaccharide synthesized by products epsI operon major virulence factor for R. . Expression has been demonstrated to be under control several proteins, including two-component regulators. Overexpression EpsR was found previously reduce amount synthesis specifically from promoter. Here we present data that a single chromosomal copy epsR activates promoter, suggesting...
Signaling by the interleukin-36 receptor (IL-36R) is linked to inflammatory diseases such as psoriasis. However, regulation of IL-36R signaling poorly understood. Activation in cultured cells results an increased polyubiquitination subunit, IL-1Rrp2. Treatment with deubiquitinases shows that subunit IL-36R, IL-1Rrp2, primarily polyubiquitinated at K63 position, which associated endocytic trafficking and signal transduction. A minor amount ubiquitination K48 position protein degradation....
A search for genes reported to interact with UBP8 and UBP10, coding two highly‐conserved ubiquitin proteases required deubiquitylation of histone H2B, revealed the yeast homologue AMPK, SNF1. Snf1 is when cellular glucose limited, as it activates stress‐responsive transcription factors. We used a mutant that lacks Ubp8 Ubp10 investigate whether regulated by ubiquitylation degradation. SNF1 mRNA levels in were no different wild type, but protein dramatically decreased. The decreased level was...
Mismatch repair is a DNA system which critical for the maintenance of genome stability. Defects in mismatch have been linked to colorectal and sporadic cancers. Calorie restriction (CR) has shown extend life span various organisms reduce tumor incidence mammals. By using Saccharomyces cerevisiae model CR aging studies, defective cells (msh2Δ, msh3Δ, msh6Δ, msh2Δmsh3Δ, msh2Δmsh6Δ msh3Δmsh6Δ) were cultured with normal (2% glucose) (0.5% medium. Here we demonstrate that containing 0.5% glucose...
Breast cancer (BC) was the 4th‐leading cause of death in Taiwan with rising incidence as malignant type young premenopausal women during recent decades. The aim our study to investigate effects physiological levels selenite on regulation BC tumor suppression gene through hypermethylation. Exposure human breast cell line MDA‐MB 231 1.5 and 2.5 μM sodium for 7 days significantly decreased invasion migration ability compared control. Selenite treatment (2.5 μM) reactivated anti‐invasion CST6...
Histone modifications are important determinants of gene expression, replication, DNA repair and cell cycle control. Ubiquitylated lysine 123 on H2B (H2Bub) is shown to contribute efficient reassembly nucleosomes during RNA polymerase II (Pol‐II)‐mediated transcription elongation in yeast. Here, we demonstrate that absence H2Bub ( htb1‐K123R mutant) causes a slowdown G1/S transition prolonged S phase as the initiation nascent synthesis defective. Significantly, control independent H3K4 K79...