A5016061803- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Nanoparticles: synthesis and applications
- Bacterial biofilms and quorum sensing
- Lanthanide and Transition Metal Complexes
- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Cardiovascular Disease and Adiposity
- Barrier Structure and Function Studies
- Antimicrobial agents and applications
- Advanced Drug Delivery Systems
- Inflammation biomarkers and pathways
- Advanced Nanomaterials in Catalysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Biomedical and Engineering Education
- Oral microbiology and periodontitis research
- Photoacoustic and Ultrasonic Imaging
- Anatomy and Medical Technology
- RNA Interference and Gene Delivery
- Computational Drug Discovery Methods
- Graphene and Nanomaterials Applications
- Career Development and Diversity
- Cancer Research and Treatment
- Dendrimers and Hyperbranched Polymers
- Engineering Education and Pedagogy
University of Vermont
2019-2024
Burlington College
2020
Binghamton University
2015-2019
University of Calgary
2011-2018
Calgary Laboratory Services
2014
Foothills Medical Centre
2011
The University of Texas at Austin
2008
Colorado State University
2004
McGill University
1999
Computed tomography (CT) is among the most popular medical imaging modalities due to its high resolution images, fast scan time, low cost, and compatibility with all patients. CT scans of soft tissues require localization contrast agents (CA) create contrast, revealing anatomic information. Gold nanoparticles (AuNP) have attracted interest recently for their use as CA X-ray attenuation, simple surface chemistry, biocompatibility. Targeting molecules may be attached particles allow targeting...
Abstract The formation of biofilms is a developmental process initiated by planktonic cells transitioning to the surface, which comes full circle when disperse from biofilm and transition mode growth. Considering that pyruvate has been previously demonstrated be required for P. aeruginosa biofilms, we asked whether likewise contributes maintenance structure, with depletion resulting in dispersion. Here, demonstrate enzymatic coincided dispersion established S. aureus laboratory clinical...
Hemodynamic factors drive nanoparticle distribution<italic>in vivo</italic>and<italic>in vitro</italic>in cell-based flow models.
Access to safe water is critical for public health. Pseudomonas aeruginosa ranked as one of the most significant bacterial pathogens, threatening human Hydrogen peroxide and other biocides are...
Intracellular calcium increased via TRPV4 in response to 20 nm gold nanoparticles, which may play an important role endothelial layer permeability.
Accurate imaging of atherosclerosis is a growing necessity for timely treatment the disease. Magnetic resonance (MRI) promising technique plaque imaging. The goal this study was to create polymeric particles small size with high loading diethylenetriaminepentaacetic acid gadolinium (III) (Gd-DTPA) and demonstrate their usefulness MRI. A water-in-oil-in-oil double emulsion solvent evaporation used encapsulate MRI agent in poly(lactide-co-glycolide) (PLGA) or polylactide-poly(ethylene glycol)...
Nanoparticles are increasingly important in medical research for application to areas such as drug delivery and imaging. Understanding the interactions of nanoparticles with cells physiologically relevant environments is vital their acceptance, cell-particle likely vary based on design particle including its size, shape, surface chemistry. For this reason, kinetic fluorescent sizes 20, 100, 200, 500 nm human umbilical vein endothelial (HUVEC) were determined by (1) measuring per cell at 37...
Abstract Nanoparticles are increasingly used in medical applications such as drug delivery, imaging, and biodiagnostics, particularly for cancer. The design of nanoparticles tumor delivery has been largely empirical, owing to a lack quantitative data on angiogenic tissue sequestration. Using fluorescence correlation spectroscopy, the deposition rate constants into blood vessel determined. It is shown that dependent surface charge. Moreover, size dependency strongly suggests taken up by...
In this work, we evaluated the impact of gold nanoparticles on endothelial cell behavior and function beyond influence viability. Five types were studied: 5 nm 20 bare nanoparticles, with biocompatible polyethylene glycol (PEG) coating 60 nanoparticles. We found that all tested did not affect viability significantly reduced reactive oxygen species (ROS) level in cells. Only caused an over 50% increase barrier permeability slow recovery was observed after removed. This impairment by...
Abstract We report on ultraviolet (UV) light induced increases in the UV optical density of thin and optically transparent crystalline DNA films formed through self assembly. The are comprised closely packed, multi-faceted sub micron sized crystals. UV-Vis spectrophotometry reveals that with surface densities up to 0.031 mg/mm 2 can reduce transmittance incident UVC UVB by 90%, UVA 20%. Subsequent independent film irradiation either or dosages upwards 80 J/cm both transmittance, reductions...
Inflammatory diseases are the leading cause of death in world, accounting for 3 out 5 deaths. Despite abundance diagnostic tools detection, most screening and methods indirect insufficient as they unable to reliably discriminate between high-risk or low-risk stages inflammatory diseases. Previously, we showed that selective activation interpolymer complexed superparamagnetic iron oxide nanoparticles (IPC-SPIOs) under oxidative conditions can be detected by a change T2 magnetic resonance (MR)...
Novel dye-linked zinc oxide nanoparticles (NPs) hold potential as photosensitizers for biomedical applications due to their excellent thermal- and photo-stability. The particles produced reactive oxygen species (ROS) upon irradiation with 850 nm near infrared (NIR) light in a concentration- time-dependent manner. Upon irradiation, ROS detected vitro human umbilical vein endothelial cells (HUVEC) carcinoma MCF7 positively correlated particle concentration interestingly, was higher than HUVEC....
Developments in mRNA/lipid nanoparticle (LNP) technology have advanced the fields of vaccinology and gene therapy, raising questions about immunogenicity. While some mRNA/LNPs generate an adjuvant-like environment muscle tissue, other are distinct their capacity for multiple rounds therapeutic delivery. We evaluate adjuvancy components by phenotyping cellular infiltrate at injection sites, tracking uptake immune cells, assessing inflammatory state. Delivery 9 common, but chemically distinct,...
Reactive oxygen species (ROS) are key markers of inflammation, with varying levels superoxide indicating the degree inflammation. Inflammatory diseases remain leading cause death in developed world. Previously, we showed that interpolymer complexed superparamagnetic iron oxide nanoparticles (IPC-SPIOs) capable decomplexing and activating T2 magnetic resonance (MR) contrast superoxide-rich environments. Here, investigate ability IPC-SPIOs to scavenge ROS immune endothelial cells which should...