A5018394344- Acute Myeloid Leukemia Research
- Monoclonal and Polyclonal Antibodies Research
- Acute Lymphoblastic Leukemia research
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Multiple Myeloma Research and Treatments
- Orthopedic Surgery and Rehabilitation
- Peripheral Nerve Disorders
- Hematopoietic Stem Cell Transplantation
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Venous Thromboembolism Diagnosis and Management
- Histone Deacetylase Inhibitors Research
- Cardiovascular Function and Risk Factors
- NF-κB Signaling Pathways
- Genetic Associations and Epidemiology
- Radiopharmaceutical Chemistry and Applications
- Neutropenia and Cancer Infections
- Neuroblastoma Research and Treatments
- Immunodeficiency and Autoimmune Disorders
- Platelet Disorders and Treatments
- Diversity and Career in Medicine
- BRCA gene mutations in cancer
Tallaght University Hospital
2017-2025
Vhi Healthcare
2024
National Cancer Institute
2024
Duke University
2024
Advocate Christ Medical Center
2023
University of Rochester
2003-2021
Gloucestershire Royal Hospital
2016-2018
University of Kansas Medical Center
2018
Oregon Health & Science University
2018
Moffitt Cancer Center
2018
Purpose CPX-351 is a dual-drug liposomal encapsulation of cytarabine and daunorubicin that delivers synergistic 5:1 drug ratio into leukemia cells to greater extent than normal bone marrow cells. Prior clinical studies demonstrated sustained exposure in vivo prolonged survival versus standard-of-care plus chemotherapy (7+3 regimen) older patients with newly diagnosed secondary acute myeloid (sAML). Patients Methods In this open-label, randomized, phase III trial, 309 age 60 75 years...
Allogeneic blood transfusion has been implicated as an independent risk factor for postoperative bacterial infection in clinical and animal studies. The association among transfusion, quantitative immunologic factors, was examined 102 patients undergoing 109 spinal fusion procedures. In 60 procedures, received autologous only; 24 they at least 1 unit of allogeneic blood, 25 no transfusions. Twenty-two developed infections, 8 cases while hospital 14 after discharge. Univariate analysis...
Adhesion of B cell precursors to accessory cells in the bone marrow microenvironment may be required for normal early development. Human adhere more avidly than mature marrow-derived fibroblasts. To determine mechanism this adhesion, expression adhesion proteins on human precursor and lines was measured by flow cytometry. The very late antigen (VLA) integrins VLA-4 VLA-5 were only expressed at higher levels cells. Antibodies alpha beta chains VLA-4, but not VLA-5, significantly blocked...
We have utilized monoclonal antibodies against the two IgG Fc receptors (p40 and p72) of U937 cells to stimulate release superoxide. The antibody (mAb) specific for p40 (IV3) has been described elsewhere. A murine IgG1 mAb high affinity p72 receptor (designated FcR32 or simply 32) bound same precipitated by Sepharose-human as shown preclearing experiments identical isoelectric focussing patterns. Binding 32 was independent region since Fab' fragments adsorbed p72. binding both human intact...
7000 Background: Older patients with secondary AML have poor outcomes following first-line cytarabine and anthracycline-based treatment. CPX-351 is a liposomal formulation of daunorubicin encapsulated at 5:1 molar ratio enhanced efficacy among risk patients. We report final results from randomized open-label study in high-risk sAML (NCT01696084). Methods: Patients 60-75 years age untreated history prior cytotoxic treatment, antecedent MDS or CMML (+/- hypomethylator treatment), WHO-defined...
We describe a newly recognized 40 kD FcR for IgG on human neutrophilic granulocytes. An mAb (IV3) developed against the of K562 cells, and specific as well monocytes platelets, was found to purify by affinity adsorption protein from detergent lysates surface-radioiodinated neutrophils. This protein, proteolytically degraded 33 when purified in absence diisopropylfluorophosphate, is distinct 51-73 precipitated anti-neutrophil mAb, 3G8, previously described others. Complete inhibition binding...
Development of lymphoid progenitors in vivo requires interaction with a bone marrow stromal microenvironment containing multiple cytokines involved the development nonlymphoid hemopoietic lineages. We tested effect one such cytokine, TGF-beta, on proliferation early human clonogenic using stroma-dependent vitro culture system. TGF-beta caused dose-dependent inhibition progenitor colonies that was reversible at low doses by addition exogenous IL-7 to cultures. unable reverse inhibitory higher...
The 40 kD monocyte Fc receptor for IgG is capable of binding murine IgG1 and supporting an anti-T3 T lymphocyte proliferative response among approximately 80% Caucasian individuals (responders), whereas the on monocytes remaining (nonresponders) incapable interacting with IgG1. By using a monoclonal antibody (mab IV3) that reacts receptor, we found receptors from responder nonresponder cannot be distinguished by either electrophoretic mobility SDS-polyacrylamide gels, or number per cell as...
Abstract Introduction Telemedicine uses communications technology for remote healthcare. Unreadiness includes difficulties with hearing, speaking, cognitive issues, vision problems, lack of internet-enabled devices, or no recent use digital communication. Telehealth can enhance access and convenience, especially rural patients, but faces challenges such as issues impacts on patient-provider relationships, examination quality, care patient satisfaction. The COVID-19 pandemic has accelerated...
Background: A point mutation in coagulation factor V (A1691G) is associated with increased risk for venous thrombosis. However, limited information available about the prospective deep thrombosis specific high-risk clinical settings. Objective: To determine whether Leiden an occurrence of patients undergoing hip or knee replacement surgery. Design: Retrospective analysis plasma samples obtained during six trials that compared different antithrombotic prophylaxis regimens Setting: Inpatients...
Abstract Evidence has recently been presented that a 40,000 dalton membrane sialoglycoprotein (p40) shared by monocytes and granulocytes serves as the human platelet receptor for aggregated IgG. We now report platelets of normal donors exhibit stable quantitative differences in expression this molecule, determined flow cytometry using fluorescent staining with murine monoclonal antibody to p40 (mab IV.3). These inter-donor were reproducible on repeated testing over at least 4 mo. In...
Three classes of FcR have been defined on human myeloid cells by their reactivity with mAb; FcRI (mAb 32); FcRII IV3); and FcRIII 3G8). We quantitated the expression each leukemia cell lines (KG-1, HL-60, U937, K562). Detailed analysis surface is provided for U937 line after exposure to CSF cytokines. Increased occurred at 72 h in exposed GCT or Mo line-conditioned medium as well from PHA-treated mononuclear cells. The augmentation required protein synthesis was diminished a neutralizing...
Abstract We compared the chemotactic responsiveness of different subsets human B lineage cells to stromal derived factor-1 (SDF-1). High percentages (30–40% input) purified bone marrow progenitors including non-B progenitors, pro-B cells, and pre-B migrated SDF-1α, demonstrating that SDF-1 is an efficacious chemoattractant these cells. Pro-B responded optimally a lower concentration than other subsets, more potent this subset. A percentage (10–15% mature lymphocytes SDF-1α decreases during...
An in vitro culture system which bone marrow-derived fibroblast-like cells support the growth of TdT+ colonies derived from CD34+/CD10- human marrow progenitor has recently been described. The regulatory role cytokines during early B lineage commitment was investigated using this system. Expression IL-7, a cytokine that induces proliferation cell precursors, detectable adherent layer by PCR and bioassay. Lymphoid were inhibited neutralizing anti-IL-7 Ab, suggesting IL-7 produced required...
Murine B cell precursors can be induced to proliferate in culture if allowed bind bone marrow derived adherent cells prepared under specific conditions. We studied the binding of human precursor subpopulations various vitro microenvironments determine which conditions may potentially suitable models for differentiation. Using markers CD10, CD34, and CD20, lineage populations increasing maturation were quantitated: CD10+/CD34+, CD10+/CD20-, CD10+/CD20+, CD10-/CD20+ marrow, mature peripheral...
Stem cell factor (SCF) is hypothesized to play a critical role in the migration of melanocytes during embryogenesis because mutations either SCF gene, or its ligand, c‐kit, result defects coat pigmentation mice and skin humans. In this report we directly show that alters adhesion human extracellular matrix (ECM) ligands regulates integrin expression at protein level. decreased neonatal fetal cells collagen IV, increased attachment laminin. Attachment fibronectin was decreased, but unchanged...