A5079500790- CRISPR and Genetic Engineering
- Nerve injury and regeneration
- Amyotrophic Lateral Sclerosis Research
- Neurobiology and Insect Physiology Research
- Genomics and Phylogenetic Studies
- SARS-CoV-2 detection and testing
- RNA and protein synthesis mechanisms
- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Sirtuins and Resveratrol in Medicine
- Signaling Pathways in Disease
- Pluripotent Stem Cells Research
- Genetics, Aging, and Longevity in Model Organisms
- Single-cell and spatial transcriptomics
- Neurogenetic and Muscular Disorders Research
- Silk-based biomaterials and applications
- Biomedical and Engineering Education
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Axon Guidance and Neuronal Signaling
- Neurological diseases and metabolism
- Biosensors and Analytical Detection
- Cell Image Analysis Techniques
Gladstone Institutes
2017-2020
University of Massachusetts Chan Medical School
2009-2018
University of California, San Francisco
2017
California Polytechnic State University
2015
Howard Hughes Medical Institute
2009
Sarm-Assisted Suicide Neurodegenerative disease or nerve lesions cause axons and synapses to disintegrate through a process known as Wallerian degeneration, which may involve an active “axon death program.” Osterloh et al. (p. 481 , published online 7 June; see the Perspective by Yu Luo ) identify loss-of-function mutations in Drosophila dSarm that are capable of blocking degeneration severed for fly life span. Deletion mouse Sarm1 provides similar protection weeks after injury, suggests...
Axon degeneration occurs in all neurodegenerative diseases, but the molecular pathways regulating axon destruction during neurodegeneration are poorly understood. Sterile Alpha and TIR Motif Containing 1 (Sarm1) is an essential component of prodegenerative pathway driving after axotomy represents appealing target for therapeutic intervention neurological conditions involving loss. Amyotrophic lateral sclerosis (ALS) characterized by rapid, progressive motor neuron muscle atrophy, causing...
SUMMARY The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an ongoing global pandemic due in part to the challenge identifying symptomatic, asymptomatic and pre-symptomatic carriers virus. CRISPR-based diagnostics that utilize RNA DNA-targeting enzymes can augment gold-standard PCR-based testing if they be made rapid, portable accurate. Here we report development amplification-free CRISPR-Cas13a-based mobile phone assay for direct detection SARS-CoV-2 from nasal...
Forward genetic screens in Drosophila melanogaster using ethyl methanesulfonate (EMS) mutagenesis are a powerful approach for identifying genes that modulate specific biological processes an vivo setting. The mapping of contain randomly-induced point mutations has become more efficient thanks to the maturation and availability many types tools. However, classic approaches gene relatively slow ultimately require extensive Sanger sequencing candidate chromosomal loci. With advent new...
Axonal and synaptic degeneration is a hallmark of peripheral neuropathy, brain injury, neurodegenerative disease. has been proposed to be mediated by an active autodestruction program, akin apoptotic cell death; however, loss-of-function mutations capable potently blocking axon self-destruction have not described. Using forward genetic screen in Drosophila , we identified that loss the Toll receptor adaptor dSarm (sterile a/Armadillo/Toll-Interleukin homology domain protein)...