A5047303234- CRISPR and Genetic Engineering
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Genomics and Phylogenetic Studies
- Marine Ecology and Invasive Species
- Animal Virus Infections Studies
- PARP inhibition in cancer therapy
- Viral gastroenteritis research and epidemiology
- Computational Drug Discovery Methods
- Evolution and Genetic Dynamics
- Aquaculture disease management and microbiota
- Virus-based gene therapy research
- Protein Degradation and Inhibitors
- Parasite Biology and Host Interactions
- Viral Infections and Immunology Research
- HIV Research and Treatment
- Autophagy in Disease and Therapy
- Sirtuins and Resveratrol in Medicine
- RNA Interference and Gene Delivery
- Calcium signaling and nucleotide metabolism
- Chromatin Remodeling and Cancer
Gladstone Institutes
2017-2021
University of California, San Francisco
2017-2021
Quantitative BioSciences
2020
Howard Hughes Medical Institute
2017-2018
University of California, Berkeley
2017-2018
Lawrence Berkeley National Laboratory
2016
Although coronavirus disease 2019 (COVID-19) causes cardiac dysfunction in up to 25% of patients, its pathogenesis remains unclear. Exposure human induced pluripotent stem cell (iPSC)-derived heart cells severe acute respiratory syndrome 2 (SARS-CoV-2) revealed productive infection and robust transcriptomic morphological signatures damage, particularly cardiomyocytes. Transcriptomic disruption structural genes corroborates adverse morphologic features, which included a distinct pattern...
Choanoflagellates, the closest living relatives of animals, can provide unique insights into changes in gene content that preceded origin animals. However, only two choanoflagellate genomes are currently available, providing poor coverage their diversity. We sequenced transcriptomes 19 additional species to produce a comprehensive reconstruction gains and losses shaped ancestral animal repertoire. identified ~1944 families originated on stem lineage, which 39 conserved across all animals our...
Recent studies have shown that molecular phylogenies of the choanoflagellates (Class Choanoflagellatea) are in disagreement with their traditional taxonomy, based on morphology, and Choanoflagellatea requires considerable taxonomic revision. Furthermore, suggest morphological ecological evolution group is more complex than has previously been recognized. Here we address taxonomy major choanoflagellate order Craspedida, by erecting four new genera. The genera to be morphologically,...
The replication of SARS-CoV-2 and other coronaviruses depends on transcription negative-sense RNA intermediates that serve as the templates for synthesis positive-sense genomic (gRNA) multiple different subgenomic mRNAs (sgRNAs) encompassing fragments arising from discontinuous transcription. Recent studies have aimed to characterize expression transcripts in order investigate their clinical significance. Here, we describe a novel panel reverse droplet digital PCR (RT-ddPCR) assays designed...
SUMMARY The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an ongoing global pandemic due in part to the challenge identifying symptomatic, asymptomatic and pre-symptomatic carriers virus. CRISPR-based diagnostics that utilize RNA DNA-targeting enzymes can augment gold-standard PCR-based testing if they be made rapid, portable accurate. Here we report development amplification-free CRISPR-Cas13a-based mobile phone assay for direct detection SARS-CoV-2 from nasal...
SUMMARY Rapid and sensitive quantification of RNA is critical for detecting infectious diseases identifying disease biomarkers. Recent direct detection assays based on CRISPR-Cas13a 1–4 avoid reverse transcription DNA amplification required gold-standard PCR 5 , but these have not yet achieved the sensitivity are easily multiplexed to detect multiple viruses or variants. Here we show that Cas13a acting single target RNAs loaded into droplets exhibits stochastic nuclease activity can be used...
Direct, amplification-free detection of RNA has the potential to transform molecular diagnostics by enabling simple on-site analysis human or environmental samples. CRISPR-Cas nucleases offer programmable RNA-guided recognition that triggers cleavage and release a fluorescent reporter molecule
Abstract The changes in gene content that preceded the origin of animals can be reconstructed by comparison with their sister group, choanoflagellates. However, only two choanoflagellate genomes are currently available, providing poor coverage diversity. We sequenced transcriptomes 19 additional species to produce a comprehensive reconstruction gains and losses shaped ancestral animal repertoire. find roughly 1,700 families origins on stem lineage, which core set 36 conserved across animals....
The origin of animals, which occurred over 600 million years ago, left no evidence in the fossil record. To trace earliest events animal prehistory, we compare extant animals to their closest living relatives, choanoflagellates, order reconstruct gene content last common ancestor and how it evolved on stem lineage leading animals. In this project, increase accuracy ancestral choanoflagellate reconstructions by sequencing transcriptomes 19 species choanoflagellates selected for phylogenetic diversity.