A5022853348- Autophagy in Disease and Therapy
- CRISPR and Genetic Engineering
- Pineapple and bromelain studies
- Acute Myeloid Leukemia Research
- Drug Transport and Resistance Mechanisms
- DNA and Nucleic Acid Chemistry
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- Tuberculosis Research and Epidemiology
- Immune Cell Function and Interaction
- Calcium signaling and nucleotide metabolism
- Vitamin D Research Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced biosensing and bioanalysis techniques
- Growth and nutrition in plants
- Macrophage Migration Inhibitory Factor
- Computational Drug Discovery Methods
- Biochemical and Structural Characterization
- Hemoglobinopathies and Related Disorders
- Genomics and Chromatin Dynamics
- Peroxisome Proliferator-Activated Receptors
- TGF-β signaling in diseases
- Garlic and Onion Studies
Children's Hospital of Philadelphia
2017-2023
University of Pennsylvania
2017-2021
Institute of Microbial Technology
2010-2020
Council of Scientific and Industrial Research
2013-2015
Mycobacterium tuberculosis-macrophage interactions are key to pathogenesis and clearance of these bacteria. Although between M. tuberculosis-associated lipids TLRs, non-TLRs, opsonic receptors have been investigated, infected macrophage lipid repertoire with lipid-sensing nuclear expressed in macrophages not addressed. In this study, we report that can interact host peroxisome proliferator-activated receptor γ testicular 4 ensure survival the pathogen by modulating function. These two create...
Macrophage derived foam cells in atherosclerotic plaques are the major factor responsible for pathogenesis of atherosclerosis (AS). During advanced AS, macrophage-specific macroautophagy/autophagy is dysfunctional. 1, 25-dihydroxy vitamin D3 (VitD3) and its receptor VDR (vitamin D receptor) reported to inhibit cell formation induce autophagy; however, role VitD3-VDR-induced autophagy AS has not been explored. Here we find that VitD3 significantly recovered oxidized low-density...
Janus kinase 2 (JAK2) is a central in hematopoietic stem/progenitor cells (HSPCs), and its uncontrolled activation prominent oncogenic driver of neoplasms. However, molecular mechanisms underlying the regulation JAK2 have remained elusive. Here we report that Casitas B-cell lymphoma (CBL) family E3 ubiquitin ligases down-regulate stability signaling via adaptor protein LNK/SH2B3. We demonstrated depletion CBL/CBL-B or LNK abrogated ubiquitination, extended half-life, enhanced cell growth...
NR1D1 (nuclear receptor subfamily 1, group D, member 1), an adopted orphan nuclear receptor, is widely known to orchestrate the expression of genes involved in various biological processes such as adipogenesis, skeletal muscle differentiation, and lipid glucose metabolism. Emerging evidence suggests that members superfamily perform a decisive role modulation autophagy. Recently, has been implicated augmenting antimycobacterial properties macrophages providing protection against Mycobacterium...
Mycobacterium tuberculosis can evade host defense processes, thereby ensuring its survival and pathogenesis. In this study, we investigated the role of nuclear receptor, pregnane X receptor (PXR), in M. infection human monocyte-derived macrophages. demonstrate that PXR augments inside macrophages by promoting foamy macrophage formation abrogating phagolysosomal fusion, inflammation, apoptosis. Additionally, cell wall lipids, particularly mycolic acids, crosstalk with (hPXR) interacting...
Mycobacterium tuberculosis, the causative agent of has a remarkable ability to usurp its host's innate immune response, killing millions infected people annually. One approach manage infection is prevention through use natural agents. In this regard, stem bromelain (SBM), pharmacologically active member sulfhydryl proteolytic enzyme family, obtained from Ananas comosus and possessing induce acquired systems, important.We evaluated SBM's apoptosis free-radical generation in macrophages. We...
GlgB (α-1,4-glucan branching enzyme) is the key enzyme involved in biosynthesis of α-glucan, which plays a significant role virulence and pathogenesis Mycobacterium tuberculosis. Because α-glucans are implicated survival both replicating non-replicating bacteria, there exists an exigent need for identification development novel inhibitors targeting enzymes, such as GlgB, this pathway. We have used existing structural information M. tuberculosis high throughput virtual screening molecular...
Abstract Fanconi anemia (FA) is a bone marrow failure (BMF) syndrome that arises from mutations in network of FA genes essential for DNA interstrand crosslink (ICL) repair and replication stress tolerance. While allogeneic stem cell transplantation can replace defective HSCs, interventions to mitigate HSC defects do not exist. Remarkably, we reveal here Lnk ( Sh2b3 ) deficiency restores function Fancd2 −/− mice. does impact ICL repair, but instead stabilizes stalled forks manner, part,...
Transcriptional enhancers can be in physical proximity of their target genes via chromatin looping. The enhancer at the β-globin locus (locus control region [LCR]) contacts fetal-type (
Hematopoietic stem cell transplantation (HSCT) remains the only curative treatment for a variety of hematological diseases. Allogenic HSCT requires hematopoietic cells (HSCs) from matched donors and comes with cytotoxicity mortality. Recent advances in genome modification HSCs have demonstrated possibility using autologous HSCT-based gene therapy to alleviate hematologic symptoms monogenic diseases, such as inherited bone marrow failure (BMF) syndrome Fanconi anemia (FA). However, FA other...