A5105976175- Plant Gene Expression Analysis
- Microbial bioremediation and biosurfactants
- Microbial Community Ecology and Physiology
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Fungal and yeast genetics research
- Bacterial biofilms and quorum sensing
- Plant Reproductive Biology
- Biofuel production and bioconversion
- Plant Pathogens and Fungal Diseases
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Forest ecology and management
- Methane Hydrates and Related Phenomena
- Biochemical and biochemical processes
- Microbial Metabolic Engineering and Bioproduction
- Isotope Analysis in Ecology
- Wood Treatment and Properties
- Plant Diversity and Evolution
- Microplastics and Plastic Pollution
- Granular flow and fluidized beds
- RNA Research and Splicing
- Marine Toxins and Detection Methods
- Microbial Natural Products and Biosynthesis
- Atmospheric and Environmental Gas Dynamics
Centre National de la Recherche Scientifique
2005-2019
Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux
2000-2019
Université Toulouse III - Paul Sabatier
2006-2014
Université de Pau et des Pays de l'Adour
2009-2014
Laboratoire de Recherche en Sciences Végétales
2005-2014
Université de Toulouse
2009-2013
John Innes Centre
1999
Université de Bordeaux
1995
• The eucalyptus R2R3 transcription factor, EgMYB1 contains an active repressor motif in the regulatory domain of predicted protein. It is preferentially expressed differentiating xylem and capable repressing two key lignin genes vivo. In order to investigate planta role this putative transcriptional biosynthetic pathway, we overexpressed gene Arabidopsis poplar. Expression produced similar phenotypes both species, with stronger effects transgenic plants than Vascular development was altered...
The presence of lignin in secondary cell walls (SCW) is a major factor preventing hydrolytic enzymes from gaining access to cellulose, thereby limiting the saccharification potential plant biomass. To understand how lignification regulated prerequisite for selecting biomass better adapted bioethanol production. Because transcriptional regulation mechanism controlling expression genes involved biosynthesis, our aim was identify novel transcription factors dictating profiles model Arabidopsis....
Summary Tension wood formed in response to gravitational force is a striking example of the plasticity angiosperm wood. In this study our goal was characterize early changes gene expression during tension formation Eucalyptus . Using cDNA array technology, transcript profiling 231 genes preferentially expressed differentiating xylem followed from 6 h 1 wk time course artificially bent trees. 196 were differentially regulated between control and trees, some exhibiting distinctive patterns...
• Eucalyptus is one of the world's main sources biomass. The genus includes species representing principle hardwood trees used for pulp and paper. Here, we aimed to identify genes specifically expressed in differentiating secondary xylem compared with phloem. We constructed a vs phloem subtractive library (Xp) that generated 263 unique sequences. By transcript profiling xylem, phloem, vascular cambium leaves using macroarrays, classified unigenes into distinct tissue-specific groups. Reverse...
Hydrophobic organic compounds (mainly lipids and hydrocarbons) represent a significant part of the matter in marine waters, their degradation has an important impact carbon fluxes within oceans. However, because they are nearly insoluble water phase, by microorganisms occurs at interface with thus requires specific adaptations such as biofilm formation. We show that Marinobacter hydrocarbonoclasticus SP17 develops biofilms, referred to oleolytic on large variety hydrophobic substrates,...
Abstract Background Renowned for their fast growth, valuable wood properties and wide adaptability, Eucalyptus species are amongst the most planted hardwoods in world, yet they still at early stages of domestication because conventional breeding is slow costly. Thus, there huge potential marker-assisted programs to improve traits such as properties. To this end, sequencing, analysis annotation a large collection expressed sequences tags (ESTs) from genes involved formation would provide...
BTEX biodegradation by an indigenous deep subsurface microbial community was evaluated in a water sample collected the area of underground gas storage. Five different sulfate-reducing communities able to use at least either benzene, toluene, ethylbenzene, or xylene (BTEX) compounds were studied. A total 21 bacterial phylotypes identified, each containing three nine phylotypes. Archaeal retrieved from only communities. The analysis 16S rRNA gene sequences showed that i) these consortia mainly...
The assimilation of the nearly water insoluble substrates hydrocarbons and lipids by bacteria entails specific adaptations such as formation oleolytic biofilms. present article reports that extracellular matrix an biofilm formed Marinobacter hydrocarbonoclasticus at n-hexadecane-water interfaces is largely composed proteins typically cytoplasmic translation factors chaperones, a lesser amount unknown function are predicted extra-cytoplasmic. Matrix appear to form structured film on...
ABSTRACT In this report, we have shown that the yeast amphiphysin-like protein Rvs167p was localized mainly in small cortical patches throughout cell unbudding cells. During budding, were polarized at bud emergence site. mating, concentrated tip of shmoo. colocalized with actin during vegetative growth and mating. Complete disruption cytoskeleton using Latrunculin-A did not affect localization cell. rvs167 mutant cells, are mislocalized rvs161 or abp1 is affected. These observations suggest...
Summary Biofilm formation by marine hydrocarbonoclastic bacteria is commonly observed and has been recognized as an important mechanism for the biodegradation of hydrocarbons. In order to colonize new oil‐water interfaces, surface‐attached communities must release cells into environment. Here we explored physiology freshly dispersed from a biofilm Marinobacter hydrocarbonoclasticus developing at hexadecane–water interface, combining proteomic physiological approaches. The comparison cells'...
Mutations in RVS161 and RVS167 yeast genes induce identical phenotypes associated to actin cytoskeleton disorders. The whole Rvs161 protein is similar the amino-terminal part of Rvs167p, thus defining a RVS domain. In addition this domain, Rvs167p contains central glycine-proline-alanine rich domain SH3 To assess function these different domains we have expressed recombinant Rvs proteins rvs mutant strains. Phenotype analysis has shown that are necessary for phenotypical complementation,...
To better understand the genetic control of secondary xylem formation in trees we analysed genes expressed during Eucalyptus development. Using eucalyptus cDNA libraries, identified EgROP1, a member plant ROP family Rho-like GTPases. These signalling proteins are central regulators many important processes plants, but information on their role xylogenesis is scarce. Quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) confirmed that EgROP1 was preferentially...
ABSTRACT This study describes the functional characterization of two proteins, AupA and AupB, which are required for growth on alkanes in marine hydrocarbonoclastic bacterium Marinobacter hydrocarbonoclasticus . The aupA aupB genes form an operon whose expression was increased upon adhesion to biofilm formation n- hexadecane. AupB outer inner membrane respectively, able interact physically. Mutations or/and reduced solid paraffin liquid hexadecane, while nonalkane substrates not affected. In...
The rvs161 and rvs167 mutant cells exhibit several identical phenotypes including sensitivity to different growth conditions morphological defects such as alteration of the actin cytoskeleton budding patterns. selection genes that, when overexpressed, are able suppress reduced viability upon carbon starvation strain, has allowed cloning SUR7 gene (Accession Number Z46729x11). We showed that suppressive ability overexpressed concerns all phenotypes. However, this suppression is only partial...