A5080994645- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Mosquito-borne diseases and control
- Neurobiology and Insect Physiology Research
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- VLSI and Analog Circuit Testing
- Insect Pest Control Strategies
- Semiconductor materials and devices
- Integrated Circuits and Semiconductor Failure Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Copper Interconnects and Reliability
- Virus-based gene therapy research
- Genetic Neurodegenerative Diseases
- Genetically Modified Organisms Research
- Embedded Systems Design Techniques
- Low-power high-performance VLSI design
- Malaria Research and Control
- Viral gastroenteritis research and epidemiology
- Induction Heating and Inverter Technology
- Ferroelectric and Negative Capacitance Devices
- GNSS positioning and interference
- Magnetic Bearings and Levitation Dynamics
- Parallel Computing and Optimization Techniques
- Transgenic Plants and Applications
Imperial College London
2021-2024
Georgetown University
2022
Cell and Gene Therapy Catapult
2002-2003
IBM (United States)
2002-2003
University of British Columbia
2001
Fondation Jean Dausset-CEPH
2001
Cold Spring Harbor Laboratory
1996-1998
University of Cambridge
1996
Harvard University
1991
Texas Instruments (United States)
1980
Disruptions in mushroom body (MB) or central complex (CC) brain structures impair Drosophila associative olfactory learning. Perturbations adenosine 3′,5′ monophosphate signaling also disrupt To integrate these observations, expression of a constitutively activated stimulatory heterotrimeric guanosine triphosphate-binding protein α subunit (Gα s *) was targeted to structures. The ability associate odors with electroshock abolished when Gα * MB, but not CC, structures, whereas sensorimotor...
Huntington's disease (HD) is a dominant neurodegenerative caused by polyglutamine (polyQ) expansion in the protein huntingtin (htt). HD pathogenesis appears to involve production of mutated N-terminal htt, cytoplasmic and nuclear aggregation abnormal activity htt interactor proteins essential neuronal survival. Before cell death, dysfunction may be an important step pathogenesis. To explore polyQ-mediated toxicity, we expressed first 57 amino acids human containing normal [19 Gln residues...
Abstract Background Population suppression gene drive has been proposed as a strategy for malaria vector control. A CRISPR-Cas9-based transgene homing at the doublesex locus ( dsxF CRISPRh ) recently shown to increase rapidly in frequency in, and suppress, caged laboratory populations of mosquito Anopheles gambiae . Here, problem formulation, an initial step environmental risk assessment (ERA), was performed simulated field releases West Africa. Methods Building on consultative workshops...
Building on an exercise that identified potential harms from simulated investigational releases of a population suppression gene drive for malaria vector control, series online workshops nine recommendations to advance future environmental risk assessment applications.
Antigen-specific suppressor T-cell hybridomas release soluble factors (TsF) in the supernatant that modulate both vivo delayed-type hypersensitivity and vitro plaque-forming cell responses an antigen-specific manner. To study relationship between receptor (TcR) TsF, we developed a series of TcR alpha- or beta- expression variants from expressed CD3-TcR alpha/beta complex. We demonstrate loss alpha but not beta mRNA was accompanied by concomitant bioactivity. Homologous transfection cDNA into...
The integration of dual damascene copper with low-k dielectric at the 0.13 /spl mu/m technology node is described. Up to five levels wiring three different metal pitches provided in a spin-on organic inter (SiLK/sup TM/ semiconductor dielectric. Dow Chemical Co.). Additional global fluorosilicate glass (FSG) two relaxed result total up eight hierarchical for enhanced BEOL performance. Successful was achieved while maintaining reliability standards. Development new advanced unit processes...
This paper describes a 1.2V high performance 0.13 /spl mu/m generation SOI technology. Aggressive ground-rules and tungsten damascene local interconnect render the densest 6T SRAM reported to date with cell area of 2.16 mu/m/sup 2/. is accomplished 248nm lithography, using optical proximity correction resolution enhancement techniques on all critical levels. Interconnect requirements are achieved by up 8 levels Cu wiring an advanced low-k interlevel dielectric.
It is now well understood that the design of signal distribution systems high speed digital machines as important circuits themselves. The integrated circuit technology today pressing back frontiers and simultaneously providing more circuitry per unit area. A system with suitable electrical characteristics for these which physically compatible size sorely needed. candidate such an application multilayer board. This paper considers problem cross coupling from line to in when excitation...
Novel genetically modified biological control products (referred to as "GM biocontrol products") are being considered address a range of complex problems in public health, conservation, and agriculture, including preventing the transmission vector-borne parasitic viral diseases well spread invasive plant animal species. These interventions involve release organisms (GMOs) into environment, sometimes with intentional dissemination modification within local population targeted species, which...
The availability of the genomic sequence malaria mosquito Anopheles gambiae has in recent years sparked development transgenic technologies with potential to be used as novel vector control tools. These rely on genome editing that confer traits able affect vectorial capacity. This can achieved by either reducing population or making mosquitoes refractory parasite infection. For any genetically modified organism is regarded for release, molecular characterization transgene and flanking sites...
A sampled-data successive approximation CMOS micropower converter, trimmed with an on-chip laser-programmed ROM, Providing differential inputs autopolarity and 50μs conversion time, will be described. Converter has a complete processor interface, including DMA.
Population suppression gene drive is currently being evaluated, including via environmental risk assessment (ERA), for malaria vector control. One such involves the dsxFCRISPRh transgene encoding (i) hCas9 endonuclease, (ii) T1 guide RNA (gRNA) targeting doublesex locus, and (iii) DsRed fluorescent marker protein, in genetically-modified mosquitoes (GMMs). Problem formulation, first stage of ERA, releases previously identified nine potential harms to environment or health that could occur,...
In this paper, we describe a high-performance 0.18 /spl mu/m logic technology with dual damascene copper metallization and dense SRAM memory. Local interconnect allows us to fabricate cells as small 3.84 mu/m/sup 2/. We demonstrate that continues exhibit performance advantages over aluminum-based technologies in generation.