A5051852736- Lipid metabolism and disorders
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Angiogenesis and VEGF in Cancer
- Retinal Diseases and Treatments
- Reproductive Biology and Fertility
- TGF-β signaling in diseases
- Venous Thromboembolism Diagnosis and Management
- Glaucoma and retinal disorders
- Reproductive System and Pregnancy
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- Lymphatic System and Diseases
- Endometriosis Research and Treatment
- Acute Ischemic Stroke Management
University of Oulu
2018-2024
Matrix Research (United States)
2018
Vascular endothelial growth factor C (VEGF-C) induces lymphangiogenesis via VEGF receptor 3 (VEGFR3), which is encoded by the most frequently mutated gene in human primary lymphedema. Angiopoietins (Angs) and their Tie receptors regulate lymphatic vessel development, mutations of ANGPT2 were recently found However, mechanistic basis Ang2 activity not fully understood. Here, we used deletion, blocking Abs, transgene induction, transfer to study how Ang2, its Tie2 receptor, Tie1 vessels. We...
The maintenance of fluid homeostasis is necessary for function the neural retina; however, little known about significance potential fluid management mechanisms. Here, we investigated angiopoietin-4 (Angpt4, also as Ang3), a poorly characterized ligand endothelial receptor tyrosine kinase Tie2, in mouse retina model. By using genetic reporter, fate mapping, and situ hybridization, found Angpt4 expression specific sub-population astrocytes at site where venous morphogenesis occurs that lower...
Purpose: Defects in the iridocorneal angle tissues, including trabecular meshwork (TM) and Schlemm's canal (SC), impair aqueous humor flow increase intraocular pressure (IOP), eventually resulting glaucoma. Activation of endothelial tyrosine kinase receptor Tie2 by angiopoietin-1 (Angpt1) has been demonstrated to be essential for SC formation, but roles other two ligands, Angpt2 Angpt4, have controversial or not yet characterized, respectively. Methods: Angpt4 expression was investigated...
ABSTRACT Effective interplay between the uterus and embryo is essential for pregnancy establishment; however, convenient methods to screen implantation success maternal uterine response in experimental mouse models are currently lacking. Here, we report 3DMOUSEneST, a groundbreaking method analyzing sites based on label-free higher harmonic generation microscopy, providing unprecedented insights into embryo–uterine dynamics during early pregnancy. The 3DMOUSEneST incorporates second-harmonic...
Male infertility is a multifactorial condition for which the underlying causes frequently remain undefined. Genetic factors have long been associated with male fertility. However, many of them are poorly or not at all characterized and their biological functions unknown. Identifying key genes behind crucial improving prognosis treatment options, as well evaluating risk passing on genetic defects through natural assisted reproductive methods to next generation. Here, we studied Coiled-coil...
<p>Visualization of basal cell surface GFP-HUVECs</p>
<p>Legends for Sup movies 1 and 2</p>
<p>Supplementary Materials and Methods</p>
<p>Supplementary Figure S1: Amino acid sequence of ANGPT2, ANGPT2443 and ANGTPT2DAP. Supplementary S2: Competitive inhibition ANGPT4-induced TIE2 phosphorylation by ANGPT2 isoforms. S3. isoforms at the retracting cell edges. S4. Generation Angpt2443 allele effects expression on retina. S5. MMTV-PyMT mammary gland tumor model. S6. Weibel-Palade bodies subendothelial space. S7. B16F10 melanoma lung colonization S8. E0771 breast cancer transplantation model.</p>
<p>Visualization of basal cell surface ANGPT2-GFP HUVECs during spreading.</p>
<div>Abstract<p>Angiopoietin-2 (ANGPT2) is a context-dependent TIE2 agonistic or antagonistic ligand that induces diverse responses in cancer. Blocking ANGPT2 provides promising strategy for inhibiting tumor growth and metastasis, yet variable effects of targeting have complicated drug development. ANGPT2<sub>443</sub> naturally occurring, lower oligomeric protein isoform whose expression increased Here, we use knock-in mouse line (mice expressing...
<div>Abstract<p>Angiopoietin-2 (ANGPT2) is a context-dependent TIE2 agonistic or antagonistic ligand that induces diverse responses in cancer. Blocking ANGPT2 provides promising strategy for inhibiting tumor growth and metastasis, yet variable effects of targeting have complicated drug development. ANGPT2<sub>443</sub> naturally occurring, lower oligomeric protein isoform whose expression increased Here, we use knock-in mouse line (mice expressing...
<p>Supplementary Figure S1: Amino acid sequence of ANGPT2, ANGPT2443 and ANGTPT2DAP. Supplementary S2: Competitive inhibition ANGPT4-induced TIE2 phosphorylation by ANGPT2 isoforms. S3. isoforms at the retracting cell edges. S4. Generation Angpt2443 allele effects expression on retina. S5. MMTV-PyMT mammary gland tumor model. S6. Weibel-Palade bodies subendothelial space. S7. B16F10 melanoma lung colonization S8. E0771 breast cancer transplantation model.</p>
<p>Legends for Sup movies 1 and 2</p>
<p>Supplementary Materials and Methods</p>
<p>Visualization of basal cell surface ANGPT2-GFP HUVECs during spreading.</p>
<p>Visualization of basal cell surface GFP-HUVECs</p>