A5081656831- Cancer, Hypoxia, and Metabolism
- Lipid metabolism and disorders
- Cancer, Lipids, and Metabolism
- Vascular Malformations Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- TGF-β signaling in diseases
- Vascular Malformations and Hemangiomas
- Atherosclerosis and Cardiovascular Diseases
- RNA Interference and Gene Delivery
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
University of Oulu
2020-2025
In-line shear stress modulation and cell microscopic analysis.
Induced pluripotent stem cell (iPSC) derived endothelial cells (iECs) have emerged as a promising tool for studying vascular biology and providing platform modelling various diseases, including those with genetic origins. Currently, primary ECs are the main source disease in this field. However, they difficult to edit limited lifespan. To study effects of targeted mutations on an endogenous level, we generated characterized iPSC model venous malformations (VMs). CRISPR-Cas9 technology was...
<p>Visualization of basal cell surface GFP-HUVECs</p>
<p>Legends for Sup movies 1 and 2</p>
<p>Supplementary Materials and Methods</p>
<p>Supplementary Figure S1: Amino acid sequence of ANGPT2, ANGPT2443 and ANGTPT2DAP. Supplementary S2: Competitive inhibition ANGPT4-induced TIE2 phosphorylation by ANGPT2 isoforms. S3. isoforms at the retracting cell edges. S4. Generation Angpt2443 allele effects expression on retina. S5. MMTV-PyMT mammary gland tumor model. S6. Weibel-Palade bodies subendothelial space. S7. B16F10 melanoma lung colonization S8. E0771 breast cancer transplantation model.</p>
<p>Visualization of basal cell surface ANGPT2-GFP HUVECs during spreading.</p>
<div>Abstract<p>Angiopoietin-2 (ANGPT2) is a context-dependent TIE2 agonistic or antagonistic ligand that induces diverse responses in cancer. Blocking ANGPT2 provides promising strategy for inhibiting tumor growth and metastasis, yet variable effects of targeting have complicated drug development. ANGPT2<sub>443</sub> naturally occurring, lower oligomeric protein isoform whose expression increased Here, we use knock-in mouse line (mice expressing...
<div>Abstract<p>Angiopoietin-2 (ANGPT2) is a context-dependent TIE2 agonistic or antagonistic ligand that induces diverse responses in cancer. Blocking ANGPT2 provides promising strategy for inhibiting tumor growth and metastasis, yet variable effects of targeting have complicated drug development. ANGPT2<sub>443</sub> naturally occurring, lower oligomeric protein isoform whose expression increased Here, we use knock-in mouse line (mice expressing...
<p>Supplementary Figure S1: Amino acid sequence of ANGPT2, ANGPT2443 and ANGTPT2DAP. Supplementary S2: Competitive inhibition ANGPT4-induced TIE2 phosphorylation by ANGPT2 isoforms. S3. isoforms at the retracting cell edges. S4. Generation Angpt2443 allele effects expression on retina. S5. MMTV-PyMT mammary gland tumor model. S6. Weibel-Palade bodies subendothelial space. S7. B16F10 melanoma lung colonization S8. E0771 breast cancer transplantation model.</p>
<p>Legends for Sup movies 1 and 2</p>
<p>Supplementary Materials and Methods</p>
<p>Visualization of basal cell surface ANGPT2-GFP HUVECs during spreading.</p>
<p>Visualization of basal cell surface GFP-HUVECs</p>