The critical determinants of the speed an invading cell front are not well known. We performed a "wound-healing" experiment that quantifies migration human peritoneal mesothelial cells over components extracellular matrix. Results were interpreted in terms Fisher's equation, which includes for modeling random motility (diffusion) and proliferation. model predicts that, after short transient, will move as traveling wave at constant speed. This is consistent with experimental findings. Using...

Moving fronts of cells are essential features embryonic development, wound repair and cancer metastasis. This paper describes a set experiments to investigate the roles random motility proliferation in driving spread an initially confined cell population. The include analysis spreading when was inhibited. Our data have been analysed using two mathematical models: lattice-based discrete model related continuum partial differential equation model. We obtain independent estimates parameter, D,...

Standard methods for quantifying IncuCyte ZOOM(™) assays involve measurements that quantify how rapidly the initially-vacant area becomes re-colonised with cells as a function of time. Unfortunately, these give no insight into details cellular-level mechanisms acting to close area. We provide an alternative method enabling us role cell motility and proliferation separately. To achieve this we calibrate standard data available from images solution Fisher-Kolmogorov model.The model is...

Wound healing is a complex process involving the delicate interaction between elements that vary widely in nature and size scales, from nanometre level, such as molecules, to cells measured micrometres, fibres with width length on both scales. Hybrid approaches, where each species represented by model an appropriate scale, have received attention recently. In this study, we provide review of earlier work hybrid models wound healing. General for element types involved dermal used research are...

Moving cell fronts are an essential feature of wound healing, development and disease. The rate at which a front moves is driven, in part, by the motility, quantified terms diffusivity D , proliferation λ . Scratch assays commonly reported procedure used to investigate motion where initial monolayer scratched, monitored over short period time, often less than 24 h. simplest way quantifying scratch assay monitor progression leading edge. Use edge data very convenient because, unlike other...

Abstract The monitoring of infection control indicators including hospital‐acquired infections is an established part quality maintenance programmes in many health‐care facilities. However, surveillance data use can be frustrated by the infrequent nature infections. Traditional methods analysis often provide delayed identification increasing occurrence, placing patients at preventable risk. application Shewhart, Cumulative Sum (CUSUM) and Exponentially Weighted Moving Average (EWMA)...

Angiogenesis, the formation of blood vessels, may be described as a process whereby capillary sprouts are formed in response to externally supplied chemical stimuli. The then develop and organize themselves into dendritic structure. Angiogenesis occurs during embryogenesis, wound healing, arthritis growth solid tumours. In this paper we present mathematical model which describes rŏle angiogenesis observed (soft-tissue) healing. We focus attention on certain principal players involved complex...

Cells respond to various biochemical and physical cues during wound-healing tumour progression. in vitro assays used study these processes are typically conducted one particular geometry it is unclear how the assay affects capacity of cell populations spread, or whether relevant mechanisms, such as motility proliferation, somehow sensitive assay. In this work we use a circular barrier characterise spreading two different geometries. Assay 1 describes tumour-like where population spreads...

Quantifying the impact of biochemical compounds on collective cell spreading is an essential element drug design, with various applications including developing treatments for chronic wounds and cancer. Scratch assays are a technically simple inexpensive method used to study spreading; however, most previous interpretations scratch qualitative do not provide estimates diffusivity, D , or proliferation rate, λ . Estimating important investigating efficacy potential treatment provides insight...

The expansion of cell colonies is driven by a delicate balance several mechanisms including motility, cell–to–cell adhesion and proliferation. New approaches that can be used to independently identify quantify the role each mechanism will help us understand how contributes process. Standard mathematical modelling describe such colony typically neglect adhesion, despite fact cell–to-cell thought play an important role. We use combined experimental approach determine diffusivity, D, strength,...