A5002482112- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Biochemical Analysis and Sensing Techniques
- Cancer-related molecular mechanisms research
- Adipokines, Inflammation, and Metabolic Diseases
- RNA Research and Splicing
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Circular RNAs in diseases
- Evolution and Paleontology Studies
- RNA regulation and disease
- Growth Hormone and Insulin-like Growth Factors
- Hypothalamic control of reproductive hormones
- Physiological and biochemical adaptations
- Ichthyology and Marine Biology
- Metabolism, Diabetes, and Cancer
- Health disparities and outcomes
- Health, psychology, and well-being
- Molecular Biology Techniques and Applications
- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Reproductive Biology and Fertility
- Fish biology, ecology, and behavior
- Diet and metabolism studies
- Biomedical and Engineering Education
Queensland University of Technology
2013-2023
Translational Research Institute
2013-2021
Genome Institute of Singapore
2021
Harvard University
2021
Princess Alexandra Hospital
2013-2019
The University of Queensland
1995-1998
Ghrelin is a novel 28-amino acid peptide identified as the endogenous ligand for GH secretagogue receptor (GHS-R). Besides its hallmark central neuroendocrine effects in control of secretion and food intake, an unexpected reproductive facet ghrelin has recently emerged because expression this molecule cognate been demonstrated rat testis. However, whether signaling system present human gonads remains to be evaluated. In study, we have assessed presence cellular location functional receptor,...
Ghrelin, the endogenous ligand for GH secretagogue receptor (GHS-R), has been primarily linked to central neuroendocrine regulation of secretion and food intake, although additional peripheral actions ghrelin have also reported. In this context, expression its cognate recently demonstrated in rat testis, suggesting a role molecule direct control male gonadal function. However, whether signaling system is present human testis remains largely unexplored. study we report cellular location...
Standard methods for quantifying IncuCyte ZOOM(™) assays involve measurements that quantify how rapidly the initially-vacant area becomes re-colonised with cells as a function of time. Unfortunately, these give no insight into details cellular-level mechanisms acting to close area. We provide an alternative method enabling us role cell motility and proliferation separately. To achieve this we calibrate standard data available from images solution Fisher-Kolmogorov model.The model is...
Abstract Purpose: There is evidence that the hormone ghrelin stimulates proliferation in PC3 prostate cancer cell line although underlying mechanism(s) remain to be determined. A novel, exon 3–deleted preproghrelin isoform has previously been detected breast and cells; however, its characterization, expression, potential function tissues are unknown. Experimental Design: Expression of was investigated lines by reverse transcription-PCR immunohistochemistry. Proliferation apoptosis assays...
Ghrelin is a multifunctional peptide hormone expressed in range of normal tissues and pathologies. It has been reported that the human ghrelin gene consists five exons which span 5 kb genomic DNA on chromosome 3 includes 20 bp non-coding first exon (20 0). The availability bioinformatic tools enabling comparative analysis finalisation genome prompted us to re-examine structure locus.We have demonstrated presence an additional novel (exon -1) 5' extensions 0 1 using silico their existence...
While oestrogen, progesterone and growth factors, including hormone (GH), are clearly implicated in the pathogenesis of breast cancer, there is now evidence that newly described ghrelin axis also involved. The aims this study were to investigate expression cancer tissues cell lines examine effect on proliferation vitro. Ghrelin its functional receptor, secretagogue receptor (GHSR) type 1a, expressed normal tissue specimens lines. In contrast, truncated GHSR 1b isoform was exclusively...
Ghrelin, the endogenous ligand of GH secretagogue receptor (GHS-R), is a newly identified, ubiquitously expressed molecule that has been involved in wide array endocrine and nonendocrine functions, including cell proliferation. In this context, our group recently reported expression ghrelin its functional receptor, GHS-R type 1a, human ovary testis as well several testicular tumors. Ovarian malignancies, however, remain unexplored. Notably, vast majority ovarian tumors derive from surface...
Abstract The bone metastasis-derived PC3 and the lymph node LNCaP prostate cancer cell lines are widely studied, having been described in thousands of publications over last four decades. Here, we report short-read whole-genome sequencing (WGS) de novo assembly (ATCC CRL-1435) (clone FGC; ATCC CRL-1740) at ∼70 × coverage. A known homozygous mutation TP53 loss PTEN were robustly identified line, whereas line exhibited a larger number putative inactivating somatic point indel mutations (and...
A set of core concepts ("big ideas") integral to the discipline physiology are important for students understand and demonstrate their capacity apply. We found poor alignment learning outcomes in programs with majors (or equivalent) from 17 Australian universities 15 developed by a team United States. The objective this project was reach Australia-wide consensus on physiology, which can be embedded curricula across universities. four-phase Delphi method employed, starting assembling Task...
Recent evidence from our research suggested the direct role of ghrelin in control testicular function. However, pattern expression and hormonal regulation gene encoding its cognate receptor (i.e., growth hormone-secretagogue [GHS-R]) male gonad remains to be fully elucidated. In this paper, overall GHS-R mRNA rat testis was compared with that functional form, namely type 1a, different developmental experimental settings. addition, cellular distribution within adult tissue assessed. Our...
Despite advancements in our understanding of the importance stress reduction achieving good health, we still only have limited insight into impact on cellular function. Recent studies suggested that exposure to prolonged psychological may alter an individual's physiological responses, and contribute morbidity mortality. This paper presents overview study protocol are using examine life stressors lifestyle factors, health-related quality novel established biomarkers midlife older Australian...
Abstract Background Recent studies have shown that GHRH antagonists inhibit prostate tumour growth and IGF‐II production both in vivo vitro. The mechanism underlying these observations is unknown, but may involve an interaction with a prostatic receptor (GHRH‐R), raising the possibility of autocrine pathway for axis prostate. Methods GHRH‐R mRNA expression was examined by RT‐PCR human cancer cell lines, authenticity PCR products confirmed Southern analysis cDNA sequencing....
Ghrelin, an n-octanoylated 28-amino-acid peptide capable of inducing GH secretion and food intake in humans rats, is the endogenous ligand for secretagogue receptor (GHS-R). Here we describe expression tissue distribution ghrelin/GHS-R axis mouse. We also report first time identification a novel mouse ghrelin mRNA variant which there complete deletion exon 4. Translation this yields protein containing alternative C-terminal domain with unique sequence. RT-PCR primers specific was used to...
Summary 1. Ghrelin is a multifunctional peptide hormone that affects various processes, including growth and insulin release, appetite regulation, gut motility, metabolism cancer cell proliferation. produced in the stomach other normal pathological types. It may act as an endocrine or autocrine/paracrine factor. 2. The present article reviews recent findings study of ghrelin its receptor suggest gene locus give rise to number functional molecules (peptides RNA transcripts) addition ghrelin....
Ghrelin is a 28 amino acid peptide hormone that expressed in the stomach and range of peripheral tissues, where it frequently acts as an autocrine/paracrine growth factor. modified by unique acylation required for to activate its cognate receptor, secretagogue receptor (GHSR), which mediates many actions ghrelin. Recently, enzyme responsible adding fatty residue (octanoyl/acyl group) third ghrelin, GOAT (ghrelin O-acyltransferase), was identified.We used cell culture, quantitative real-time...