Abstract The molecular identification and characterization of phytoplasmas from infected grapevines in four locations Serbia are reported. Phytoplasmas were detected identified by restriction fragment length polymorphism (RFLP) analysis polymerase chain reaction (PCR) amplified 16S rDNA. Grapevine yellows associated with three molecularly distinguishable phytoplasmas: Flavescence dorée (elm group: 16SrV‐C subgroup) present only the Župa Aleksandrovac region; Bois noir (stolbur 16SrXII‐A...

Different species of the genus Monilinia are common plant pathogens that endanger pome and stone fruit production worldwide. In Serbia, two this widely distributed - M. laxa fructigena, while fructicola, which is officially on A2 EPPO List quarantine pest organisms in Europe 1A part I has so far been detected only stored apple nectarine fruits. The most important control measures against these include chemical combination with adequate cultural practices, particularly under favourable...

Brown rot is one of the most important pre- and postharvest fungal diseases stone fruit worldwide. In Serbia, where production economically important, Monilinia laxa M. fructigena are widely distributed. surveys from 2011 to 2013, 288 isolates spp. were collected 131 localities in 16 districts six hosts Serbia. Using multiplex polymerase chain reaction, phylogenetic analysis, morphological characterization, three species identified as causal agents brown fruit: (89% isolates), (3%),...

Tomato production worldwide is affected by numerous plant virus species. The early and accurate detection of viruses a critical step for disease control. However, the simultaneous most known tomato can be difficult because high number diversity tomato-infecting viruses. Here, we have identified four new in Serbia applying target-independent small RNA high-throughput sequencing (HTS). HTS was applied on pools samples separate samples, total comprising 30 that exhibited (severe) virus-like...

Considerable damping-off of the seedlings several commercial Apiaceae plant species was observed in Serbia. The infection a total 48 seed samples nine vegetable and spice plants with phytopathogenic Alternaria spp. established using deep-freeze-blotter method. Identification performed both conventional methods PCR. Four different plant-pathogenic were detected Serbia: A. dauci, radicina, petroselini, alternata, all which caused reduction carrot, parsley, parsnip, celery emergence....

In a survey to determine the presence and distribution of Iris yellow spot virus (IYSV) in greenhouse ornamentals onion field crops 14 districts Serbia as well on imported ornamental plants, 1,574 samples were collected analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). IYSV was not detected nearly 1,200 plant from 39 genera grown greenhouses or other countries during 2005 2007. The an seed crop Sirig locality (South Bačka District) that showed symptoms...

Viruses are responsible for numerous epidemics in different crops all parts of the world. As a consequence their presence great economic losses being incurred. In addition to development sensitive techniques detection, identification and characterization viruses, substantial attention has also been paid biotechnological methods elimination from plants. this review article, following vitro culture virus ornamental plants presented: meristem culture, thermotherapy, chemotherapy, cryotherapy or...

In a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities Vojvodina Province Central Serbia, 380 samples were collected analyzed by DAS-ELISA. Out seven tested, tomato spotted wilt virus (TSWV), potato Y (PVY), mosaic (TMV), cucumber (CMV), alfalfa (AMV) detected 37.9, 33.4, 28.7, 23.9, 15.5% total tested samples, respectively. TSWV was most frequently found while PVY CMV frequent Province. Single infections prevalent years 2005-2007 those...

In August 2011, nectarine (Prunus persica (L.) Batsch var. nucipersica (Suckow) C. K. Schneid) fruit originated from Oplenac region with symptoms of rot was collected at a green market in Belgrade. Fruit had large, brown, sunken lesions covered grayish brown tufts. Symptoms resembled those caused by species Monilinia including M. laxa, fructigena, or fructicola (2). order to isolate the causal organism, small superficial fragments pericarp were superficially disinfected commercial bleach and...

A survey was conducted in 2012 and 2013 to detect the presence distribution of Alfalfa mosaic virus (AMV) lavandin crops growing continental parts Croatia. total 73 samples from six different localities were collected analyzed for AMV Cucumber (CMV) using commercial double-antibody sandwich (DAS)-ELISA kits. detected serologically 62 at three localities, none tested positive CMV. For further analyses, selected naturally infected plants originating mechanically transmitted test plants:...

In June 2011, extensive bleaching and numerous small whitish spots on leaves were observed in an onion (Allium cepa) seed crop as well chlorotic streaks the neighboring garlic (A. sativum) bulb Aleksandrovo locality (Central Banat District, Serbia). Affected plants occurred throughout field disease incidence was estimated at 60% 40% crop. A high population of Thrips tabaci that found both crops, local necrotic Petunia × hybrida mechanically inoculated with infected or sap by a chilled 0.01 M...

Abstract BACKGROUND: Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense‐related proteins which, among others, include certain protease inhibitors. It is particular relevance investigate natural for pathogen control which cystatins—specific inhibitors cysteine proteases. RESULTS: In this study, a proteinase inhibitor (CPI), 11 kDa size, was purified from green kiwifruit homogeneity....

In May 2009, approximately 30% of plants within a greenhouse-grown Gerbera hybrida crop in Vranjska Banja (Pčinj District) Serbia displayed chlorotic oak-leaf patterns followed by necrosis and distortion leaves. Symptoms on naturally infected gerbera local necrotic spots Petunia × mechanically inoculated with sap using chilled 0.05 M phosphate buffer (pH 7) containing 1 mM Na-EDTA, 5 Na-DIECA, Na-thioglycolate (4) suggested the presence Tospovirus. Symptomatic leaves were tested for Tomato...

Strawberry (Fragaria × ananassa Duch.) is the third most important berry crop in Serbia with average production ranging from 30,000 to 35,000 t on approximately 5,000 ha (2). In June 2013, symptoms of wilt and whole plant collapse were observed 25% plants growing commercial strawberry cv. Alba locality Zablaće (Moravica district). Initial included leaf chlorosis wilt, followed by withering necrosis older leaves reduced fruit production, eventually leading desiccations. Internal vascular...

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During a cucurbit disease survey in August 2004, severe symptoms resembling those caused by viruses were observed on bottlegourd (Lagenaria siceraria (Molina) Standl.) the Vojvodina region of Serbia. Symptoms included stunting, mosaic, green veinbanding, blistering, yellowing, chlorotic spots, leaf deformation, and fruit distortion. Leaf samples from 25 symptomatic plants collected two localities for virus identification using mechanical transmission serological testing. Crude sap extract...

Lavandin (Lavandula × intermedia Emeric ex Loiseleur) is cultivated on a large scale in some South European countries for the extraction of essential oils or as an ornamental plant gardens and landscapes. In May 2012, virus-like symptoms including bright yellow calico mosaic, leaf distortion, growth reduction were observed 15% lavandin plants commercial nursery Banovo Brdo locality, Baranja County, Republic Croatia. Leaves from 15 symptomatic collected examined by double-antibody sandwich...

In May 2010, Impatiens walleriana plants with single or double flowers that were showing symptoms resembling those of downy mildew collected in a greenhouse the vicinity Mionica, Kolubara District, Serbia. Diseased severely stunted, mild inconspicuous mottling and yellowing on upper surface leaves. The lower affected leaves was completely covered distinctive thick, white fungal-like growth. Symptomatic wilted very quickly premature leaf fall common, leaving only few youngest no poorly...

Iris yellow spot virus (IYSV; genus Tospovirus, family Bunyaviridae) is established in several European countries (France, Italy, The Netherlands, Poland, Slovenia, Spain, and the UK) its distribution EU region has increased since 2002 (3). In July 2007, symptoms resembling those of IYSV were observed an onion (Allium cepa) seed crop Sirig locality Serbia. Onion plants exhibited characteristic chlorotic or necrotic spindle diamond-shaped lesions on leaves scapes. Symptomatic found throughout...

In July 2011, greenhouse-grown chrysanthemum hybrid plants (Chrysanthemum × morifolium) with symptoms resembling those associated tospoviruses were observed in the Kupusina locality (West Bačka District, Serbia). Disease incidence was estimated at 40%. Symptomatic chlorotic ring spots and line patterns sampled tested by double antibody sandwich (DAS)-ELISA using polyclonal antisera (Bioreba AG, Reinach, Switzerland) against two of most devastating greenhouse floriculture industry: Tomato...