Fatty acids were identified in monofloral beebread (BB) and bee pollen (BP) loads collected from Trifolium pratense L. A gas chromatography method was used to identify quantify fatty acids: Thirty-five BB 42 BP. high amount of the healthy n-3 found. The ratio polyunsaturated n-6 reached a value 8.42 3.35 latter products. proteomic analysis also performed on manually T. pollen, most abundant protein groups subjected mass spectrometry analysis. Proteins are involved main cellular functions...

ABSTRACT Oxygen insensitive glucose biosensors based on PQQ-dependent dehydrogenase from Erwinia sp. 34-1 and carbon paste have been designed. Without special precautions the sensitivity of with immobilized enzyme was low. Several methods immobilization were compared. The in polylysine-albumin gel a consisting water insoluble organic mediator, chemically modified powder, fumed silica binding material. Chemical modification quinonic mediators application considerably improves reproducibility,...

Abstract In the study, honey from oilseed rape Brassica napus L., and both hand-collected (winter Visby Cult) bee-collected pollen of were analyzed for their proteome content, in order to see if any plant proteins present allow proteo-typing honey. Proteins fractionated by two-dimensional gel electrophoresis (2DE), stained Coomassie blue then mass spectrometry. All identified divided into few groups due biological function. 2DE gels with separated blossom honey, only bee (Apis mellifera)...

The aim of the present work was to describe most unique pollen found in honey collected protected landscapes Lithuania.Honey samples were from Lithuania's landscape areas study botanical origin pollen.A total 17 obtained different geographical locations Lithuania: Varduva Scenic Landscape Reserve, Salantai Regional Park, Žemaitija National Dzūkija border between Poland and Lithuania Lazdijai District, Armona Geological Gomerta Krekenava Park.Botanical determined by melissopalynology...

Clovers are widely distributed, but their pollen proteome still has not been completely elucidated.In this study we performed a comprehensive comparative proteomic analysis of red, berseem and white clover pollen.Handcollected different species were used for total protein extraction.Proteins isolated from red cvs.'Kiršiniai' 'Vyčiai', cv.'Faraon' cv.'Medūnai', populations Nos.2295 4n 2196 4n, subjected to fractionation by one-dimensional electrophoresis.Some quantitatively groups...

Abstract Glucose sensitive biosensor containing pyrroloquinoline quinone (PQQ)‐dependent glucose dehydrogenase immobilized on Prussian blue (PB)‐modified graphite electrode was designed. Properties of the were investigated in cathodic and anodic response detection regions. It shown, that is sum two signals: direct electron transport from reduced PQQ to by formation PQQ‐oxygen‐PB‐carbon ternary complex. Cathodic based oxidation PB‐oxygen‐PB Electrochemical regeneration enzyme does not produce...

The aim of this study was to develop mixtures bee bread and pollen with linseed oil, sea buckthorn royal jelly, algae Spirulina (Spirulina platesnis) determine the impact vegetable oils on invertase glucose oxidase activity, free acidity influence storage conditions enzyme activity in tested products. duration determined by evaluating correlation between products known botanical origin supplemented additives. additives bioactive properties 14 combinations pure pollen. highest relationship (r...

Proteins from hand- and bee- collected pollen were isolated separated in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) two-dimensional (2DE) system as well by direct gel-free mass spectrometry (MS) analysis. Total thirty six plant proteins with known functions three uncharacterized identified using techniques fourteen protein spots 2D electrophoresis. Our study revealed two forms of profilin their slight different 14.19 kDa 14.09 beta actin fragment was 33.25 both...

The study reports a first comprehensive approach to the proteome analysis done by comparing protein profiles characteristic of bee- and manually-collected common dandelion (Taraxacum officinale) pollen. Proteins isolated from pollen was fractionated two-dimensional electrophoresis, visualized staining with Colloidal Coomassie G-250, after tryptic-digestion analysed mass spectrometry. A computer-assisted performed for comparison profile maps typical

The bioreactor for the production of dihydroxyacetone phosphate (DHAP) was developed by immobilization α-glycerophosphate oxidase (GPO) on Prussian Blue-(PB) modified carbon fibre (CF) electrode. DHAP reached 32.0 mM with a yield 64 ± 5% from 50 L-α-glycerophosphate (GP), while maximum concentration hydrogen peroxide did not exceed 2 mM. A conjugation two catalytic activities, enzymatic oxidation GP to GPO and electrochemical elimination PB, allowed exclusion catalase biocatalytic process....

FAD containing dehydrogenases could donate electrons to Prussian Blue coated electrodes and operate in the absence of oxygen. The same electrode also be used for reduction hydrogen peroxide produced at low potentials or oxidation higher potentials. These features were demonstrated using a glycerol phosphate oxidase as an example. next group carbon was electro-oxidation products urea hydrolysis catalyzed by urease. Three different mechanisms action proposed depending on potential....