A5011710956- Electrochemical sensors and biosensors
- Bacteriophages and microbial interactions
- Enzyme Catalysis and Immobilization
- Electrochemical Analysis and Applications
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Amino Acid Enzymes and Metabolism
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Microbial metabolism and enzyme function
- Microbial bioremediation and biosurfactants
- Analytical Chemistry and Sensors
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Metal-Catalyzed Oxygenation Mechanisms
- Polyamine Metabolism and Applications
- Viral gastroenteritis research and epidemiology
- Microbial Community Ecology and Physiology
- Enzyme-mediated dye degradation
- Microfluidic and Capillary Electrophoresis Applications
- Microbial Metabolites in Food Biotechnology
- Metalloenzymes and iron-sulfur proteins
- Plant and Fungal Interactions Research
Vilnius University
2016-2025
Biotechnology Institute
2024
Institute of Biochemistry
1999-2008
University of Leicester
2000-2002
University of Cambridge
2002
Auto-antibodies with specificity to self-antigens have been implicated in a wide variety of neurological diseases, including Parkinson's (PD) and Alzheimer's being sensitive indicators neurodegeneration focus for disease prevention. Of particular interest are the studies focused on auto-immune responses amyloidogenic proteins associated diseases their applications therapeutic treatments such as vaccination amyloid antigens antibodies PD, potentially other ailments.Generated auto-antibodies...
Comparison of conventional and newly developed laccase-based electrocatalytic systems for oxygen reduction.
At 346 kbp in size, the genome of a jumbo bacteriophage vB_KleM-RaK2 (RaK2) is largest Klebsiella infecting myovirus sequenced to date. In total, 272 out 534 RaK2 ORFs lack detectable database homologues. Based on similarity biologically defined proteins and/or MS/MS analysis, 117 were given functional annotation, including 28 coding for structural that have no reliable homologues annotated other organisms. The electron micrographs revealed elaborate spike-like structures tail fibers Rak2,...
Indole is a molecule of considerable biochemical significance, acting as both an interspecies signal and building block biological elements. Bacterial indole degradation has been demonstrated for number cases; however, very little known about genes proteins involved in this process. This study reports the cloning initial functional characterization (iif ant cluster) responsible biodegradation Acinetobacter sp. strain O153. The catabolic cascade was reconstituted vitro with recombinant...
Enzymatic degradation of plastic pollution offers a promising environmentally friendly waste management strategy, however, suitable biocatalysts must be screened and developed. Traditional screening methods using soluble or solubilised polymers do not necessarily identify enzymes that are effective against solid crystalline polymers. This study presents simple, time-saving cost-effective method for identifying microorganisms capable degrading polymeric films. The was tested on...
The reaction of heterotetrameric sarcosine oxidase (TSOX) Arthrobactor sp. 1-IN has been studied by stopped-flow spectroscopy, with particular emphasis on the reduction enzyme sarcosine. Expression cloned gene encoding TSOX in Escherichia coli enables production a scale suitable for studies. Treatment sulfite provides means selective formation flavin−sulfite adduct covalent 8α-(N3-histidyl)-FMN. Formation sulfite−flavin suppresses internal electron transfer between noncovalent FAD (site...
ABSTRACT Acidithiobacillus caldus has been proposed to play a role in the oxidation of reduced inorganic sulfur compounds (RISCs) produced industrial biomining sulfidic minerals. Here, we describe regulation new cluster containing gene encoding tetrathionate hydrolase ( tetH ), key enzyme RISC metabolism this bacterium. The contains five cotranscribed genes, IS ac1 , rsrR rsrS and doxD coding for transposase, two-component response regulator (RsrR RsrS), hydrolase, DoxD, respectively. As...
A range of diseases is associated with amyloid fibril formation. Despite different proteins being responsible for each disease, all them share similar features including beta-sheet-rich secondary structure and fibril-like protein aggregates. number can form amyloid-like fibrils in vitro, resembling structural disease-related amyloids. Given these generic properties fibrils, inhibitors formation would be interest treatment diseases. Recently, we identified five outstanding insulin among the...
By using a self-assembled amyloid from lysozyme as biotemplate we produced an ultrathin silver wire of 1 nm diameter and up to 2 mum in length, which is at the limit attainable nanobiotechnological manufacturing. We showed that 2,2,2-trifluoroethanol produces dual effect: it reduces ionic colloidal nanoparticles with regular size, depending on length incubation, induces fibrillar assembly into scaffold, forming hollow channel filled silver.
ABSTRACT De novo protein design for catalysis of any desired chemical reaction is a long standing goal in engineering, due to the broad spectrum technological, scientific and medical applications. Currently, mapping sequence function is, however, neither computationionally nor experimentally tangible 1,2 . Here we developed ProteinGAN, specialised variant generative adversarial network 3 that able ‘learn’ natural diversity enables generation functional sequences. ProteinGAN learns...
Semi-permeable capsules offers a powerful approach to isolate and process thousands of single bacterial cells simultaneously.
To maximize the potential of 5G infrastructure in healthcare, simple integration biosensors with wireless tag antennas would be beneficial. This work introduces novel glucose-to-resistor transduction, which enables simple, biosensor design. The was realized on a near-field communication antenna, where sensing bioanode generated electrical current and electroreduced nonconducting antenna material into an excellent conductor. For this, part replaced by Ag nanoparticle layer oxidized to...
Abstract In this study, we present an extensive analysis of a widespread group bacterial tRNA de-modifying enzymes, dubbed RudS, which consist TudS desulfidase fused to Domain Unknown Function 1722 (DUF1722). RudS enzymes exhibit specific de-modification activity towards the 4-thiouridine modification (s4U) in molecules, as indicated by our experimental findings. The heterologous overexpression genes Escherichia coli significantly reduces content and diminishes UVA-induced growth delay,...
The nucleotide sequences of two cloned DNA fragments containing the structural genes heterotetrameric sarcosine oxidase ( soxBDAG ) and dimethylglycine dehydrogenase dmg from Arthrobater spp. 1‐IN Arthrobacter globiformis , respectively, have been determined. Open reading frames were identified in operon corresponding to four subunits by comparison with N‐terminal amino‐acid subunit relative molecular masses purified enzyme. Alignment deduced sequence functionally related proteins indicated...