Auto-antibodies with specificity to self-antigens have been implicated in a wide variety of neurological diseases, including Parkinson's (PD) and Alzheimer's being sensitive indicators neurodegeneration focus for disease prevention. Of particular interest are the studies focused on auto-immune responses amyloidogenic proteins associated diseases their applications therapeutic treatments such as vaccination amyloid antigens antibodies PD, potentially other ailments.Generated auto-antibodies...

Peripheral immune responses can be sensitive indicators of disease pathology. We evaluated the autoimmune reactions to endocrine (insulin) and astrocytical (S100B) biomarkers in blood sera 26 Parkinson's (PD) patients compared with controls by using ELISA. found a statistically significant increase both antigens PD mean 70% 50% towards insulin S100B, respectively. Heterogeneity observed may reflect modulating effect multiple variables associated neurodegeneration also changes basic...

The nucleotide sequences of two cloned DNA fragments containing the structural genes heterotetrameric sarcosine oxidase ( soxBDAG ) and dimethylglycine dehydrogenase dmg from Arthrobater spp. 1‐IN Arthrobacter globiformis , respectively, have been determined. Open reading frames were identified in operon corresponding to four subunits by comparison with N‐terminal amino‐acid subunit relative molecular masses purified enzyme. Alignment deduced sequence functionally related proteins indicated...

The biodegradation pathway of pyridine, a notorious toxicant, is relatively unexplored, as no genetic data related to this process have ever been presented. In paper, we describe the plasmid-borne pyr gene cluster, which includes complete set genes responsible for degradation pyridine. A key enzyme, monooxygenase PyrA, first step catabolic pathway, performs an oxidative cleavage pyridine ring without typical activation steps such reduction or hydroxylation heterocycle. This work provides new...

Typical laccases have four copper atoms, which form three different centers, of the T1 is responsible for blue color enzyme and gives it a characteristic absorbance around 610 nm. Several unusual spectral properties are referred to as yellow or white laccases. Only two from Ascomycota phylum been described previously, only one amino acid sequence those enzymes available. A laccase Bcl1 Botrytis cinerea strain 241 has identified, purified characterized in this work. The appears be dimer with...

A cryptic plasmid from Arthrobacter rhombi PRH1, designated as pPRH, was sequenced and characterized. It 5000 bp in length with a G+C content of 66 mol%. The pPRH predicted to encode six putative open reading frames (ORFs), which ORF2 ORF3 formed the minimal replicon shared 55–61% 60–69% homology, respectively, RepA RepB proteins reported rhodococcal plasmids. Sequence analysis revealed typical ColE2-type ori located 45 upstream gene repA. phylogenetic led conclusion that is representative...

Bacterial strain 68b was isolated from contaminated soil. According to 16S rDNA analysis it belongs genus Arthrobacter. This is capable utilise phthalic acid as a sole carbon source. ability proved by physiological and biochemical tests. By using resting cells, found out that Arthrobacter sp. cells could use or convert quinolinic if they were pre-grown in the presence of acid. While analysing results partially sequenced genome, putative phthalate degradation operon (pht) detected. It...

The propensity of peptides and proteins to form self-assembled structures has very promising applications in the development novel nanomaterials. Under certain conditions, amyloid protein α-synuclein forms well-ordered – fibrils, which have proven be valuable building blocks for bionanotechnological approaches. Herein we demonstrate functionalization fibrils formed by a mutant that contains an additional cysteine residue. been biotinylated via thiol groups subsequently joined with...

Bionanoparticles comprised of naturally occurring monomers are gaining interest in the development novel drug transportation systems. Here we report on stabilisation, cellular uptake, and macrophage clearance nanotubes formed from self-assembling gp053 tail sheath protein vB_EcoM_FV3 bacteriophage. To evaluate potential bacteriophage protein-based as therapeutic nanocarriers, investigated their internalisation into colorectal cancer cell lines professional macrophages that may hinder...

Equine lysozyme (EL) is a calcium (Ca)-binding and an intermediary link between non-Ca-binding C-type α-lactalbumin. The feature of lysozymes to assemble into the fibrils has recently gained considerable attention for investigation functional properties these proteins. To study structural EL, synthetic gene was cloned EL overexpressed in Escherichia coli as fused protein. His-tagged recombinant accumulated inclusion bodies. Up 50 mg/l could be achieved after purification by Ni2+ affinity...

Aldonic acids have already found many applications in food, pharmaceutical, and cosmetics industries there are new ones likely to come.

Laccases are important and valuable enzymes with a great potential for biotechnological applications. In this study, two novel laccases, LacHU1 LacHU2, from Alternaria sp. HU have been purified characterized. The molecular mass of each isoenzyme was ~66 kDa. laccases yellow had no typical blue oxidase spectra LacHU2 color characteristic absorption spectra. catalytic efficiency most substrates higher than that laccase. Both isoenzymes effectively oxidize flavonoids. were successfully...

Abstract Here, we present a proof‐of‐principle for new high‐throughput functional screening of metagenomic libraries the selection enzymes with different activities, predetermined by substrate being used. By this approach, total 21 enzyme‐coding genes were selected, including members xanthine dehydrogenase, aldehyde dehydrogenase (ALDH), and amidohydrolase families. The system is based on pro‐chromogenic substrate, which transformed target enzyme to indole‐3‐carboxylic acid. later compound...

The aim of this work was to investigate the application artificial electron transfer mediators for pyrroloquinoline quinone-dependent soluble glucose dehydrogenase (PQQ-sGDH) catalyzed lactose oxidation reaction. 1-(N,N-dimethylamine)4-(4-morpholine)benzene (AMB) and N,N’-dimethyl-4,4’-azopyridinium methyl sulfate (MAZP) were used as mediators. showed a high reactivity with reduced form PQQ-sGDH determine parameters at pH 7.0 8.0. use MAZP AMB regeneration oxidized PQQsGDH investigated by...

We report on the construction of functionalized nanotubes based tail sheath protein 041 from vB_KleM-RaK2 bacteriophage. The truncated (041Δ200) was fused with fluorescent proteins GFP and mCherry or amidohydrolase YqfB. generated chimeric were successfully synthesized in E. coli BL21 (DE3) cells self-assembled into tubular structures. detected fluorescence structures, which confirmed by stimulated emission depletion microscopy. When 041Δ200GFP 041Δ200mCherry coexpressed cells, formed...

Based on modification of beta-sheeted protein structures in biosynthesis stage selfassemblage 3D solid state surfaces is investigated. A set hybrid proteins containing the abeta40 peptide domain are constructed and characterized. The extended biomolecular immobilised surfaces. Self assembled surface obtained by deposition conglomerates from buffer solutions. mica, SiO2, SnO In2O3 covered with layer. Formation fibrils dependent immobilisation period origin surface. Morphology, mechanical...