A5014847774- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Cancer Research and Treatments
- Plant Disease Resistance and Genetics
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Animal Disease Management and Epidemiology
- Plant Virus Research Studies
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
Vilnius University
2020-2023
Achromobacter spp. are ubiquitous in nature and increasingly being recognized as emerging nosocomial pathogens. Nevertheless, to date, only 30 complete genome sequences of phages available GenBank, nearly all those were isolated on xylosoxidans. Here, we report the isolation characterization bacteriophage vB_AchrS_AchV4. To best our knowledge, vB_AchrS_AchV4 is first virus from spanius. Both its host, spanius RL_4, Lithuania. VB_AchrS_AchV4 a siphovirus, since it has an isometric head (64 ±...
Klebsiella pneumoniae poses a major global challenge due to its virulence, multidrug resistance, and nosocomial nature. Thus, bacteriophage-derived proteins are extensively being investigated as means combat this bacterium. In study, we explored the enzymatic specificity of depolymerase gp531, encoded by jumbo bacteriophage vB_KleM_RaK2 (RaK2). We used two different methods modify reducing end oligosaccharides released during capsule hydrolysis with gp531. Subsequent acidic cleavage TFA,...
Shiga toxin-producing
The structures of the Caudovirales phage tails are key factors in determining host specificity these viruses. However, because enormous structural diversity, molecular anatomy recognition apparatus has been elucidated only a number phages. Klebsiella viruses vB_KleM_RaK2 (RaK2) and phiK64-1, which form new genus Alcyoneusvirus according to ICTV, have perhaps one most structurally sophisticated adsorption complexes all tailed described date. Here, gain insight into early steps alcyoneusvirus...
We report on the construction of functionalized nanotubes based tail sheath protein 041 from vB_KleM-RaK2 bacteriophage. The truncated (041Δ200) was fused with fluorescent proteins GFP and mCherry or amidohydrolase YqfB. generated chimeric were successfully synthesized in E. coli BL21 (DE3) cells self-assembled into tubular structures. detected fluorescence structures, which confirmed by stimulated emission depletion microscopy. When 041Δ200GFP 041Δ200mCherry coexpressed cells, formed...
Bacteria of the genus Klebsiella pose significant global challenges due to their virulence, multidrug resistance, and nosocomial nature. Thus, potential bacteriophage-derived proteins combat these pathogens is currently extensively being investigated. In this study, enzymatic specificity virion-associated depolymerase gp531, encoded by pneumoniae jumbo bacteriophage vB_KleM_RaK2 (RaK2), was determined means two different experimental approaches for modifying reducing ends oligosaccharides...