A5014147815- Pesticide and Herbicide Environmental Studies
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Enzyme Catalysis and Immobilization
- RNA and protein synthesis mechanisms
- Phytase and its Applications
- Environmental Toxicology and Ecotoxicology
- Animal Virus Infections Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Genomics and Phylogenetic Studies
- Heavy metals in environment
- Animal Disease Management and Epidemiology
- Bacteriophages and microbial interactions
- Biotin and Related Studies
- Enzyme Structure and Function
- Reproductive Biology and Fertility
- Heat shock proteins research
- Salmonella and Campylobacter epidemiology
- Enzyme-mediated dye degradation
- Animal Genetics and Reproduction
- Heavy Metal Exposure and Toxicity
- Trace Elements in Health
- Protein Hydrolysis and Bioactive Peptides
- Machine Learning in Bioinformatics
- Quality and Safety in Healthcare
Harbin Veterinary Research Institute
2016-2024
Chinese Academy of Agricultural Sciences
2011-2024
Nanjing Agricultural University
2023-2024
Anqing Normal University
2022-2024
Huazhong Agricultural University
2023-2024
Yuhuangding Hospital
2021
Anhui Sanlian University
2020
Biotechnology Research Institute
2008-2019
Beijing National Laboratory for Molecular Sciences
2017
Peking University
2013-2017
As the most abundant liver-specific microRNA, miR-122 is involved in diverse aspects of hepatic function and neoplastic transformation. Our previous study showed that levels are significantly decreased hepatitis B virus (HBV)-infected patients, which may facilitate viral replication persistence (S. Wang, L. Qiu, X. Yan, W. Jin, Y. Chen, E. Wu, Ye, G. F. Gao, Z. Duan, S. Meng, Hepatology 55:730-741, 2012). Loss expression patients with enhances through cyclin G1-modulated P53 activity.). In...
Protein thermostability can be increased by some glycine to proline mutations in a target protein. However, not all improve protein thermostability, and this method is suitable only at carefully selected mutation sites that accommodate structural stabilization. In study, homology modeling molecular dynamics simulations were used select appropriate the effect of was proved experiments. The structure methyl parathion hydrolase (MPH) from Ochrobactrum sp. M231 (Ochr-MPH) constructed modeling,...
Abstract Background para -Nitrophenol (PNP), a priority environmental pollutant, is hazardous to humans and animals. However, the information relating PNP degradation pathways their enzymes remain limited. Results Pseudomonas sp.1-7 was isolated from methyl parathion (MP)-polluted activated sludge shown degrade PNP. Two different intermediates, hydroquinone (HQ) 4-nitrocatechol (4-NC) were detected in catabolism of This indicated that degraded by two pathways, namely HQ pathway,...
Abstract A promoter that enabled high-level expression of the target gene during stationary phase in absence an inducer would facilitate efficient production heterogeneous proteins at a low cost. In this study, genome-scale microarray-based approach was employed to identify promoters induced genes Bacillus subtilis from late log without inducer. Eleven candidate were selected based on B. microarray data and quantitative PCR analysis. Among promoters, P ylb exhibited highest activity with...
Abstract Background An important aspect of protein design is the ability to predict changes in thermostability arising from single- or multi-site mutations. Protein reflected change free energy (ΔΔ G ) thermal denaturation. Results We have developed predictive software, Prethermut, based on machine learning methods, effect mutations thermostability. The input vector Prethermut known structural and empirical measurements potential due Using a 10-fold cross validation test M-dataset,...
Redox imbalance in cells induces lipid peroxidation and generates a class of highly reactive metabolites known as lipid-derived electrophiles (LDEs) that can modify proteins affects their functions. Identifying targets LDEs is critical to understand how such modifications are functionally implicated oxidative-stress associated diseases. Here we report quantitative chemoproteomic method globally profile protein sites modified by LDEs. In this strategy, designed synthesized an...
Of the 20 common amino acids, 18 are encoded by multiple synonymous codons. These codons not redundant; in fact, all of contribute substantially to protein expression, structure and function. In this study, codon usage pattern genes E. coli was learned from sequenced genomes coli. A machine learning based method, Presyncodon proposed predict selection on patterns residue context specific fragment. The predicting results indicate that Presycoden could be used gene with high accuracy. Two...
Abstract Equine infectious anemia (EIA) is a contagious disease of horses caused by the equine virus (EIAV). The clinical signs at acute phase include intermittent high fever, thrombocytopenia, hemorrhage, edema, and anemia. chronic relapsing subclinical levels emaciation progressive weakness. Surviving become lifelong carriers because integration viral genome into that host, these can produce transmit to other animals. This increases difficulty imposing practical control measures prevent...
Extracellular α-amylase from Pyrococcus furiosus (PFA) shows great starch-processing potential for industrial application due to its thermostability, long half-life and optimal activity at low pH; however, it is difficult produce in large quantities. In contrast, Bacillus amyloliquefaciens (BAA) can be produced larger quantities, but lower stability high temperatures pH. Here, we describe a BAA protein expression pattern-mimicking strategy express PFA B. using the secretion elements of BAA,...
Cadmium (Cd) is a heavy metal that extremely toxic to many organisms; however, microbes are highly adaptable extreme conditions, including contamination. Bacteria can evolve in the natural environment, generating resistant strains be studied understand resistance mechanisms, but obtaining such adaptive usually takes long time. In this study, genome replication engineering-assisted continuous evolution (GREACE) method was used accelerate evolutionary rate of Escherichia coli screen for E....
Salmonella enterica subsp. serovar Abortusequi is a major pathogen in horse and donkey herds, causing abortion pregnant equids resulting enormous economic losses. A rapid reliable method urgently needed to detect S .
The administration of NAD+ precursors is a potential approach to protect against liver damage and metabolic dysfunction. However, the effectiveness different in alleviating disorders still poorly elucidated. current study was performed compare four precursors, including nicotinic acid (NA), niacinamide (NAM), nicotinamide riboside (NR), mononucleotide (NMN) high-glucose-induced injury hepatocytes fish model, Megalobrama amblycephala. An vitro high-glucose model successfully established mimic...
Pullulanase (EC 3.2.1.41) plays an important role in the specific hydrolysis of branch points amylopectin. Enhancing its thermostability is required for industrial application. In this study, rational protein design was used to improve PulB from Bacillus naganoensis (AB231790.1), which has strong enzymatic properties. Three positive single-site mutants (PulB-D328H, PulB-N387D, and PulB-A414P) were selected six mutants. After incubation at 65°C 5 min, residual activities PulB-D328H,...
Emerging evidence highlights the regulatory role of paired-like (PRD-like) homeobox transcription factors (TFs) in embryonic genome activation (EGA). However, majority PRD-like genes are lost rodents, thus prompting an investigation into TFs other mammals. Here, we showed that were transiently expressed during EGA human, monkey, and porcine fertilized embryos, yet they exhibited inadequate expression their cloned embryos. This study, using pig as research model, identified LEUTX a key...
Abstract Fluorine is ubiquitous and the most active non-metal element in nature. While many microorganisms have developed fluoride resistance as a result of widespread prolonged application oral hygiene products, mechanisms used by these organisms to overcome toxicity are incompletely understood. In this study, fluoride-resistant strain, Enterobacter cloacae FRM, was identified which could grow well at concentration 4,000 mg/L. According comparative genomics, transcriptome under stress,...