A5045896799- Plant-Microbe Interactions and Immunity
- Ginseng Biological Effects and Applications
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Research in Cotton Cultivation
- Food Quality and Safety Studies
- Plant Pathogens and Fungal Diseases
- Polysaccharides and Plant Cell Walls
- Nuts composition and effects
- Pharmacological Effects of Natural Compounds
- Toxin Mechanisms and Immunotoxins
- Animal Virus Infections Studies
- Transgenic Plants and Applications
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Protein Interaction Studies and Fluorescence Analysis
- Herpesvirus Infections and Treatments
- Tea Polyphenols and Effects
- Photosynthetic Processes and Mechanisms
- Viral gastroenteritis research and epidemiology
- Phytochemicals and Antioxidant Activities
Kunming University of Science and Technology
2016-2025
Harbin Veterinary Research Institute
2014-2024
Chinese Academy of Agricultural Sciences
2020-2024
State Administration of Traditional Chinese Medicine of the People's Republic of China
2017-2022
Kunming Institute of Zoology
2011-2013
Chinese Academy of Sciences
2011-2013
Northeast Agricultural University
2010-2012
Huazhong Agricultural University
2005-2009
The root rot mainly caused by Fusarium solani is a bottleneck in the cultivation of Panax notoginseng. In this study, we reported gene encoding plant cell wall structural protein, P. notoginseng proline-rich protein (PnPRPL1), whose transcription was upregulated F. and induced some hormone signals. PnPRPL1 recombinant significantly inhibited growth conidial germination pathogens. Downregulation RNA interference (RNAi) leaves increased susceptibility to solani, whereas overexpression tobacco...
Abstract Lilies (genus Lilium) play a significant role in the global cut-flower industry, but they are highly susceptible to fusarium wilt caused by Fusarium oxysporum. However, Lilium regale, wild lily species, exhibits remarkable resistance F. To investigate quantitative of L. regale wilt, comprehensive multi-omics analysis was conducted. Upon inoculation with oxysporum, roots showed accumulation phenylpropane metabolites, including lignin precursors, flavonoids, and hydroxycinnamic acids....
Up to now, there has been no report on the taxonomic and functional analysis of microbial community in fermenting puer tea by pyrosequencing. In this study, metagenomic pyrosequencing was first used delineate a relatively comprehensive overview taxonomy while also preliminarily characterising ontologies genes present pile fermentation.A total 251 738 reads (9197 contigs 145 402 singletons) were generated Taxonomic revealed three dominant bacterial phyla, Actinobacteria (30.08%),...
The interactions between trypsin and gallic acid (GA) were investigated by means of fluorescence spectroscopy, UV-vis absorption resonance light scattering (RLS) synchronous enzymatic inhibition assay. It was found that GA can cause the quenching during process formation GA-trypsin complex, resulting in activity (IC50 = 3.9 × 10-6 mol/L). spectroscopic data showed efficiency reach about 80%. binding constants 1.9371 104 L/mol, 1.8192 1.7465 L/mol at three temperatures, respectively....
Pathogenesis-related proteins (PRs) are a class of that accumulate in response to biotic and abiotic stresses protect plants from damage. In this study, gene encoding PR-like protein (PnPR-like) was isolated Panax notoginseng , which is used traditional Chinese herbal medicines. An analysis expression P. indicated PnPR-like responsive an infection by the root rot pathogen Fusarium solani . The induced several signaling molecules, including methyl jasmonate, ethephon, hydrogen peroxide,...
Panax notoginseng (Burk.) F. H. Chen is a Chinese medicinal plant of the Araliaceae family used for treatment cardiovascular and cerebrovascular diseases in Asia. P. vulnerable to root rot disease, which reduces yield notoginseng. In this study, we analyzed rhizosphere soil endophyte microbial communities from different geographical locations using high-throughput sequencing. Our results revealed that community was more diverse than community. Rhodopseudomonas, Actinoplanes, Burkholderia,...
Recent developments in Fourier transform infrared spectroscopy-partial least squares (FTIR-PLSs) extend the application of this strategy to field edible oils and fats research. In work, FT-IR spectroscopy was used as an effective analytical tool determine peroxide value virgin walnut oil (VWO) samples undergone during heating. The spectra were recorded from a film pure between two disks KBr for each sample at frequency regions 4000–650 cm −1 . Changes values most bands observed build...
Gallic acid is one of the important polyphenols in plants and it inhibits α-amylase. The interaction between gallic α-amylase was investigated by fluorescence quenching spectroscopy, UV-vis synchronous three-dimensional spectroscopy under mimic physiological conditions. result emission at different temperatures revealed that there are static intrinsic induced a complex acid-α-amylase formed. results obtained from evaluation spectra, spectra suggested association did change molecular...
Panax notoginseng has been extensively used as a traditional Chinese medicine. In the current study, molecular cloning and characterization of PnbHLH1 transcription factor were explored in notoginseng. The full length gene obtained by splicing was 1430 bp, encoding 321 amino acids. Prokaryotic expression vector pET-28a-PnbHLH1 constructed transferred into BL21 prokaryotic strain. An electrophoretic mobility shift assay protein binding to E-box cis-acting elements verified that belonged bHLH...
Panax notoginseng (Burk) F.H. Chen is a rare and valuable Chinese herb, but root rot mainly caused by Fusarium solani severely affects the yield quality of P. herbal materials. In this study, we isolated 30 WRKY transcription factors (TFs), which were divided into three groups (I, II, III) on basis phylogenetic analysis. The expression levels 10 genes, including PnWRKY9, in roots increased response to methyl jasmonate (MeJA) treatment following F. infection. Additionally, PnWRKY9 was...
Panax notoginseng (Burk) F. H. Chen is a valuable traditional Chinese medicinal plant, but its commercial production seriously affected by root rot caused some pathogenic fungi, including Fusarium solani. Nevertheless, the genetic breeding for disease resistance of P. remains limited. The WRKY transcription factors have been revealed to play important roles in plant defense responses, which might provide an inspiration improvement notoginseng.In this study, regulatory mechanism factor...