A5101927407- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Plant Gene Expression Analysis
- Cancer-related gene regulation
- Plant-Microbe Interactions and Immunity
- Heat shock proteins research
- Plant tissue culture and regeneration
- DNA and Nucleic Acid Chemistry
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Pain Mechanisms and Treatments
- Exercise and Physiological Responses
- Biochemical and biochemical processes
- Genomics and Phylogenetic Studies
- Plant Parasitism and Resistance
- Herbal Medicine Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Plant Genetic and Mutation Studies
- Genetic diversity and population structure
- Ion Channels and Receptors
- Developmental Biology and Gene Regulation
- Histone Deacetylase Inhibitors Research
- melanin and skin pigmentation
- Cancer Treatment and Pharmacology
Kunming University of Science and Technology
2021-2025
Sun Yat-sen Memorial Hospital
2022-2025
Sun Yat-sen University
2022-2025
Ministry of Education of the People's Republic of China
2024
University of Science and Technology of China
2024
Beijing Institute of Fashion Technology
2023
Institute of Biophysics
2022
Chinese Academy of Sciences
2022
Guangzhou Medical University
2020
Guangzhou First People's Hospital
2020
Recently emerged SARS-CoV-2 Omicron subvariant, BA.2.75, displayed a growth advantage over circulating BA.2.38, BA.2.76, and BA.5 in India. However, the underlying mechanisms for enhanced infectivity, especially compared with BA.5, remain unclear. Here, we show that BA.2.75 exhibits substantially higher affinity host receptor angiotensin-converting enzyme 2 (ACE2) than other variants. Structural analyses of spike shows its decreased thermostability increased frequency binding domain (RBD)...
Abstract Known ribozymes in contemporary biology perform a limited range of chemical catalysis, but vitro selection has generated species that catalyze broader chemistry; yet, there have been few structural and mechanistic studies selected ribozymes. A ribozyme recently can site-specific methyl transfer reaction. We solved the crystal structure this at resolution 2.3 Å, showing how RNA folds to generate very specific binding site for donor substrate. The immediately suggests catalytic...
Abstract Lilies (genus Lilium) play a significant role in the global cut-flower industry, but they are highly susceptible to fusarium wilt caused by Fusarium oxysporum. However, Lilium regale, wild lily species, exhibits remarkable resistance F. To investigate quantitative of L. regale wilt, comprehensive multi-omics analysis was conducted. Upon inoculation with oxysporum, roots showed accumulation phenylpropane metabolites, including lignin precursors, flavonoids, and hydroxycinnamic acids....
Abstract Ribozymes are excellent systems in which to study ‘sequence - structure function’ relationships RNA molecules. Understanding these may greatly help structural modeling and design of functional structures some modules could be repurposed molecular design. At present, there is no comprehensive database summarising all the natural ribozyme families. We have therefore created Ribocentre, a that collects together sequence, mechanistic data on 21 This includes available information...
Abstract A methyltransferase ribozyme (MTR1) was selected in vitro to catalyze alkyl transfer from exogenous O6-methylguanine (O6mG) a target adenine N1, and recently, high-resolution crystal structures have become available. We use combination of classical molecular dynamics, ab initio quantum mechanical/molecular mechanical (QM/MM) alchemical free energy (AFE) simulations elucidate the atomic-level solution mechanism MTR1. Simulations identify an active reactant state involving protonation...
Lilium are highly economically valuable ornamental plants that susceptible to Fusarium wilt caused by oxysporum. regale Wilson, a wild lily native China, is resistant F. In this study, WRKY transcription factor, WRKY11, was isolated from L. regale, and its function during the interaction between oxysporum characterized. The resistance of LrWRKY11 ectopic expression tobacco increased, moreover, transcriptome sequencing UHPLC-MS/MS analysis indicated methyl salicylate jasmonate levels rose in...
Abstract Riboswitches are regulatory elements found in the untranslated regions (UTRs) of certain mRNA molecules. They typically comprise two distinct domains: an aptamer domain that can bind to specific small molecules, and expression platform controls gene expression. work by undergoing a conformational change upon binding their ligand, thus activating or repressing genes downstream. This mechanism allows regulation response metabolites To systematically summarise riboswitch structures...
LIM homeodomain transcription factor 1‐alpha (LMX1a) is a neuronal lineage‐specific activator that plays an essential role during the development of midbrain dopaminergic (mDA) neurons. LMX1a induces expression multiple key genes, which ultimately determine morphology, physiology, and functional identity mDA This function dependent on its homeobox domain. Here, we determined structures domain in complex with promoter sequences Wnt family member 1 ( WNT1 ) or paired like 3 Pitx3 gene,...
The Agrobacterium-based transgenic technique is commonly used for gene function validation and molecular breeding. However, it not suitable plants with a low regeneration capacity or transformation rate, such as Panax notoginseng (Burk) F.H. Chen Lilium regale Wilson. In this study, novel Agrobacterium method based on injection in the meristems was developed using P. L. experimental models. PCR analysis confirmed successful integration of reporter DsRed2 (Discosoma striata red fluorescence...
Root rot, mainly caused by Fusarium oxysporum, is the most destructive disease affecting lily (Lilium spp.) production. The WRKY transcription factors (TFs) have important roles during plant immune responses. To clarify effects of TFs on defense responses to pathogens, a gene (LrWRKY2) was isolated from Lilium regale Wilson, which wild species highly resistant F. oxysporum. expression LrWRKY2, encodes nuclear protein, induced various hormones (methyl jasmonate, ethephon, salicylic acid, and...
WRKY transcription factors (TFs) play vital roles in plant growth and development, secondary metabolite synthesis, response to biotic abiotic stresses. In a previous transcriptome sequencing analysis of Lilium regale Wilson, we identified multiple TFs that respond exogenous methyl jasmonate treatment lily Fusarium wilt (Fusarium oxysporum).In the present study, TF LrWRKY3 was further analyzed reveal its function defense F. oxysporum. The protein localized cell nucleus, transgenic tobacco...
Abstract Chemotherapy‐induced neuropathic pain is a common dose‐limiting side effect of cancer treatment but the underlying mechanisms are largely unknown. Here, we used whole‐genome expression microarray and gene ontology analysis to identify upregulation sequence‐specific DNA‐binding protein, HOXA6, in spinal dorsal horn on Day 10 after injection rats with oxaliplatin. Genetic disruption HOXA6 siRNAs alleviated mechanical allodynia oxaliplatin administration. Reduced representation...
Background: Vitiligo is an acquired depigmentation skin disorder mainly caused by the destruction of melanocytes. There are many therapeutic options available for vitiligo, but not uniformly effective.Objectives: This study aimed to explore clinical effect autologous non-cultured epidermal cell suspension (NCES) technique in treatment patients with stable vitiligo.Methods: A retrospective before-after comparisons was undertaken 41 vitiligo who received NCES technique. The percentage...
Although the action mechanism of antineoplastic agents is different, oxaliplatin, paclitaxel, or bortezomib as first-line drugs can induce painful neuropathy. In rodents, mechanical allodynia a common phenotype neuropathy for 3 chemotherapeutics. However, whether there molecular involved in different chemotherapeutics-induced peripheral remains unclear.Mechanical was tested by von Frey hairs following i.p. injection vehicle, Sprague-Dawley rats. Reduced representation bisulfite sequencing...
k-Junctions are elaborated forms of kink turns with an additional helix on the nonbulged strand, thus forming a three-way helical junction. Two were originally identified in structures Arabidopsis and Escherichia coli thiamine pyrophosphate (TPP) riboswitches, another called DUF-3268 was tentatively from sequence information. In this work we show that E. riboswitch k-junctions fold response to addition magnesium or sodium ions, atomic mutations should disrupt key hydrogen bonding...
Abstract Objectives This study aimed to develop and validate a predictive nomogram for diagnosing radicular grooves (RG) in maxillary lateral incisors (MLIs), integrating demographic information, anatomical measurements, Cone Beam Computed Tomography (CBCT) data diagnose the RG MLIs based on clinical observation before resorting CBCT scan. Materials Methods A retrospective cohort of orthodontic patients from School Hospital Stomatology, Wuhan University, was analyzed, including...
This study aimed to explore the role of histone methyltransferase SET and MYND domain containing 3 (SMYD3) in bone metabolism osteoblasts exposed fluoride. The levels urine fluoride, BALP, OC mRNA expression SMYD3 were determined patients with skeletal fluorosis non-fluoride-exposed people on informed consent. protein, contents, BALP activities detected human osteoblast-like MG63 cells rat primary treated sodium fluoride (NaF) for 48 h. autophagosomes observed by transmission electron...
Abstract X-ray crystallography is a fundamental technique that provides atomic-level insights into RNA structures. However, obtaining crystals of structures diffracting to high resolution challenging. We introduce simple strategy enhance the limit by selective substitution Watson–Crick pairs GU within sequences. Our approach has successfully yielded high-resolution for eight unique crystals. Notably, six instances showed marked enhancement upon GC/AU base pair substitution, with two cases...