A5100358541- SARS-CoV-2 and COVID-19 Research
- Renal Diseases and Glomerulopathies
- Angiogenesis and VEGF in Cancer
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Respiratory Support and Mechanisms
- Amino Acid Enzymes and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Bacillus and Francisella bacterial research
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and disorders
- Gut microbiota and health
- Reproductive biology and impacts on aquatic species
- CAR-T cell therapy research
- Tryptophan and brain disorders
- Lung Cancer Treatments and Mutations
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- Diet and metabolism studies
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
Huazhong University of Science and Technology
2024-2025
Union Hospital
2024-2025
Anhui Agricultural University
2024
Ministry of Agriculture and Rural Affairs
2023-2024
Northwestern University
2016-2024
Renal Research Institute
2018-2024
Chinese Academy of Sciences
2021-2024
Children's Hospital of Fudan University
2020-2024
Institute of Biophysics
2021-2024
Shanghai Chenshan Plant Science Research Center
2022-2024
The SARS-CoV-2 Omicron variant with increased fitness is spreading rapidly worldwide. Analysis of cryo-EM structures the spike (S) from reveals amino acid substitutions forging interactions that stably maintain an active conformation for receptor recognition. relatively more compact domain organization confers improved stability and enhances attachment but compromises efficiency viral fusion step. Alterations in local conformation, charge, hydrophobic microenvironments underpin modulation...
Abstract Omicron (B.1.1.529), the most heavily mutated SARS-CoV-2 variant so far, is highly resistant to neutralizing antibodies, raising concerns about effectiveness of antibody therapies and vaccines 1,2 . Here we examined whether sera from individuals who received two or three doses inactivated vaccine could neutralize authentic Omicron. The seroconversion rates antibodies were 3.3% (2 out 60) 95% (57 for had 2 3 vaccine, respectively. For recipients doses, geometric mean neutralization...
Recently emerged SARS-CoV-2 Omicron subvariant, BA.2.75, displayed a growth advantage over circulating BA.2.38, BA.2.76, and BA.5 in India. However, the underlying mechanisms for enhanced infectivity, especially compared with BA.5, remain unclear. Here, we show that BA.2.75 exhibits substantially higher affinity host receptor angiotensin-converting enzyme 2 (ACE2) than other variants. Structural analyses of spike shows its decreased thermostability increased frequency binding domain (RBD)...
Significance Reducing vascular leakage and stabilizing the endothelium through activation of angiopoietin (ANGPT)–TIE2 receptor tyrosine kinase pathway is a promising therapeutic strategy for diseases. ANGPT2 one two major ligands TIE2 receptor. Uniquely, possesses an agonistic role in lymphatic endothelium, but acts as competitive antagonist blood endothelium. The molecular basis opposing actions these beds poorly understood. Here we demonstrate that absence VEPTP expression confers agonist...
Primary congenital glaucoma (PCG) is a severe disease characterized by developmental defects in the trabecular meshwork (TM) and Schlemm's canal (SC), comprising conventional aqueous humor outflow pathway of eye. Recently, heterozygous loss function variants TEK ANGPT1 or compound TEK/SVEP1 were identified children with PCG. Moreover, common ANGPT1and SVEP1 have been as risk alleles for primary open angle (POAG) GWAS studies. Here, we show tissue-specific deletion Angpt1 Svep1 from TM causes...
Carboxylesterases (CEs) are widely distributed in the body and catalyze hydrolysis of endogenous exogenous substrates. They play important roles drug metabolism, abnormal levels often result numerous diseases such as cancers. Therefore, it is to establish a highly selective sensitive probe for real-time sensing CEs activity. Herein, we synthesized novel fluorescence-electrochemical multichannel 1 containing ferrocene unit, which not only serves part structure but also provides...
ABSTRACT Selective pressures have given rise to a number of SARS-CoV-2 variants during the prolonged course COVID-19 pandemic. Recently evolved differ from ancestors in additional glycosylation within spike protein receptor-binding domain (RBD). Details how acquisition impacts viral fitness and human adaptation are not clearly understood. Here, we dissected role N354-linked glycosylation, acquired by BA.2.86 sub-lineages, as RBD conformational control element attenuating infectivity. The...
Abstract With the emergence of Omicron variant, number pediatric Coronavirus Disease 2019 (COVID‐19) cases requiring hospitalization and developing severe or critical illness has significantly increased. Machine learning multivariate logistic regression analysis were used to predict risk factors develop prognostic models for COVID‐19 in hospitalized children with variant this study. Of 544 including 243 301 mild groups, respectively. Fever (92.3%) was most common symptom, followed by cough...
Aims: Emodin is an anthraquinone with potential anti-inflammatory properties. However, the possible molecular mechanisms and protective effects of emodin are not clear. The objective this study was to investigate on lipopolysaccharide (LPS)-induced acute liver injury (ALI) via Toll-like receptor 4 (TLR4) signaling pathway in Raw264.7 cell line Balb/c mice. Methods: This established inflammatory cellular model induced ALI animal model. TLR4 overexpressed by lentivirus downregulated small...
Glutarate is a five carbon platform chemical produced during the catabolism of L-lysine. It known that it can be catabolized through glutaryl-CoA dehydrogenation pathway. Here, we discover Pseudomonas putida KT2440 has an additional glutarate catabolic pathway involving L-2-hydroxyglutarate (L-2-HG), abnormal metabolite from 2-ketoglutarate (2-KG). In this pathway, CsiD, Fe2+/2-KG-dependent hydroxylase, capable converting into L-2-HG, and LhgO, L-2-HG oxidase, catalyze 2-KG. We construct...
Abstract Emergence of variants concern (VOC) with altered antigenic structures and waning humoral immunity to SARS-CoV-2 are harbingers a long pandemic. Administration third dose an inactivated virus vaccine can boost the immune response. Here, we have dissected immunogenic profiles antibodies from 3-dose vaccinees, 2-dose vaccinees convalescents. Better neutralization breadth VOCs, expeditious recall long-lasting response bolster in warding off COVID-19. Analysis 171 complex neutralizing...
Abstract Recently emerged SARS-CoV-2 Omicron subvariant, BA.2.75, displayed a local growth advantage over BA.2.38, BA.2.76 and BA.5 in India. The underlying mechanism of BA.2.75’s enhanced infectivity, especially compared to BA.5, remains unclear. Here, we show that BA.2.75 exhibits substantially higher ACE2-binding affinity than BA.5. Also, spike shows decreased thermostability increased “up” RBD conformation acidic conditions, suggesting low-pH-endosomal cell-entry pathway utilization. is...
Abstract The bat coronaviruses (CoV) BANAL-20-52 and BANAL-20-236 are two newly identified severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) closely related (SC2r-CoV) the genome of shares highest homology with SARS-CoV-2. However, risk their potential zoonotic transmission has not been fully evaluated. Here, we determined host susceptibility among 13 different species 26 animal species, found that both might have extensive ranges, indicating high potential. We also cryo-EM...
Significance Statement Ischemia-reperfusion AKI (IR-AKI) is common and causes significant morbidity. Effective treatments are lacking. However, preclinical studies suggest that inhibition of angiopoietin-Tie2 vascular signaling promotes injury, whereas activation Tie2 protective. We show kidney ischemia leads to increased levels the endothelial-specific phosphatase endothelial protein tyrosine (VE-PTP; PTPRB), which inactivates Tie2. Activation through VE-PTP deletion, or delivery a novel...
5-Aminovalerate is a potential C5 platform chemical for synthesis of valerolactam, 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. It metabolite l-lysine catabolism through the aminovalerate pathway in Pseudomonas putida. L-Lysine monooxygenase (DavB) 5-aminovaleramide amidohydrolase (DavA) play key roles biotransformation L-lysine into 5-aminovalerate. Here, DavB DavA P. putida KT2440 were expressed, purified, coupled production 5-aminovalerate from L-lysine. Under optimal conditions,...