A5100442945- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- RNA Research and Splicing
- Hippo pathway signaling and YAP/TAZ
- Polyamine Metabolism and Applications
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Pancreatic function and diabetes
- Monoclonal and Polyclonal Antibodies Research
- Glutathione Transferases and Polymorphisms
- Wnt/β-catenin signaling in development and cancer
- Genomics and Rare Diseases
- Fungal Biology and Applications
- Genetic Associations and Epidemiology
- Protein Kinase Regulation and GTPase Signaling
- Olfactory and Sensory Function Studies
- Enzyme Structure and Function
- Advanced Adaptive Filtering Techniques
- Regulation of Appetite and Obesity
- Blood disorders and treatments
- vaccines and immunoinformatics approaches
Shenzhen Bay Laboratory
2023-2024
Institute for Biomedicine
2023-2024
Griffith University
2019-2024
University of Science and Technology of China
2023-2024
Chinese Academy of Sciences
2009-2023
University of California System
2022
High Magnetic Field Laboratory
2019-2021
University of Chinese Academy of Sciences
2019-2021
Hefei Institutes of Physical Science
2019-2021
Harbin Medical University
2021
Abstract There are many differences in brain structure and function between males females. However, how these were manifested during development maintained through adulthood still unclear. Here we present a time series analyses of genome-wide transcription profiles the human brain, identified genes showing sex biased expression at major developmental stages (prenatal time, early childhood, puberty adulthood). We observed great number (>2,000 genes) between-sex divergence all with greatest...
Background Spermine synthase (SMS) is a key enzyme controlling the concentration of spermidine and spermine in cell. The importance SMS manifested by fact that single missense mutations were found to cause Snyder-Robinson Syndrome (SRS). At same time, currently there are no non-synonymous nucleoside polymorphisms, nsSNPs (harmless mutations), SMS, which may imply does not tolerate amino acid substitutions, i.e. mutable. Methodology/Principal Findings To investigate mutability we carried out...
Obesity is one of the largest health problems facing world today. Although twin and family studies suggest about two-thirds obesity caused by genetic factors, only a small fraction this variance has been unraveled. There are still large numbers genes to be identified that cause variations in body fatness associated diseases encompassed metabolic syndrome (MetS). A locus near sequence tagged site (STS) marker D6S1009 linked or mass index (BMI). However, its entity unknown. located intergenic...
Partitioning-defective 3 (Par3), a key component of the evolutionarily conserved polarity PAR complex (Par3/Par6/aPKC), controls cell and contributes to migration, proliferation tumor development. Emerging evidence indicates that proteins function as upstream modulators regulate Hippo pathway. However, little is known about Par3's involvement in Here, we find Par3 YAP dynamically co-localize different subcellular compartments; is, membrane, cytoplasm nucleus, cell-density-dependent manner....
Abstract Despite the large number of noncoding RNAs in human genome and their roles many diseases include cancer, we know very little about them due to lack structural clues. The centerpiece clues is full RNA base-pairing structure secondary tertiary contacts that can be precisely obtained only from costly time-consuming 3D determination. Here, performed deep mutational scanning self-cleaving CPEB3 ribozyme by error-prone PCR showed a library <5 × 104 single-to-triple mutants...
C-terminal Src kinase (Csk) that functions as an essential negative regulator of family tyrosine kinases (SFKs) interacts with tyrosine-phosphorylated molecules through its homology 2 (SH2) domain, allowing it targeting to the sites SFKs and concomitantly enhancing activity. Identification additional Csk-interacting proteins is expected reveal potential signaling targets previously undescribed Csk. In this study, using a direct proteomic approach, we identified 151 novel Csk-binding...
ABSTRACT Tertiary structures of RNAs are increasingly found to be essential for their functions and yet notoriously challenging determined by experimental techniques or predicted energy-based AlphaFold2-like deep-learning-based computational methods. Here, we coupled deep mutational scanning with mobility-based selection separate structurally stable from nonstable RNA variants. The subsequent high-throughput sequencing allows the detection covariational signals key secondary tertiary base...
CPEB3 ribozyme is a self-cleaving RNA that occurs naturally in mammals and requires divalent metal ions for efficient activity. Ribozymes exhibit preferences specific ions, but the exact differences catalytic mechanisms of various on remain unclear. Our findings reveal Mn2+ functions as more effective cofactor catalysis compared to Mg2+, confirmed by its stronger binding affinity EPR. Cleavage assays mutants molecular docking analyses further showed excessive can bind second site near site,...
<title>Abstract</title> Despite their importance in a wide range of living organisms, self-cleaving ribozymes the human genome are few and poorly studied. Here, we performed deep mutational scanning covariance analysis two previously proposed (LINE-1 OR4K15). We found that regions essential for ribozyme activities made short segments, with total 35 31 nucleotides only. The discovery makes them simplest known ribozymes. Moreover, circular permutated nearly identical catalytic internal loops,...
Despite their importance in a wide range of living organisms, self-cleaving ribozymes the human genome are few and poorly studied. Here, we performed deep mutational scanning covariance analysis two previously proposed (LINE-1 OR4K15). We found that regions essential for ribozyme activities made short segments, with total 35 31 nucleotides only. The discovery makes them simplest known ribozymes. Moreover, circular permutated nearly identical catalytic internal loops, supported by stems...
Despite their importance in a wide range of living organisms, self-cleaving ribozymes the human genome are few and poorly studied. Here, we performed deep mutational scanning covariance analysis two previously proposed (LINE-1 OR4K15). We found that regions essential for ribozyme activities made short segments, with total 35 31 nucleotides only. The discovery makes them simplest known ribozymes. Moreover, circular permutated nearly identical catalytic internal loops, supported by stems...
Abstract X-ray crystallography is a fundamental technique that provides atomic-level insights into RNA structures. However, obtaining crystals of structures diffracting to high resolution challenging. We introduce simple strategy enhance the limit by selective substitution Watson–Crick pairs GU within sequences. Our approach has successfully yielded high-resolution for eight unique crystals. Notably, six instances showed marked enhancement upon GC/AU base pair substitution, with two cases...
ABSTRACT High-resolution protein structure determination by experimental techniques is notoriously costly and labor intensive. This problem mostly solved with arrival of deep-learning-based computational prediction AlphaFold2 but only for those proteins enough naturally occurring homologous sequences. Here, we attempt to close the remaining gap employing artificially generated, structure-stability-selected sequences as an input AlphaFold2. We showed that one round selection deeply mutated a...
Despite their importance in a wide range of living organisms, self-cleaving ribozymes the human genome are few and poorly studied. Here, we performed deep mutational scanning covariance analysis two previously proposed (LINE-1 OR4K15). We found that regions essential for ribozyme activities made short segments, with total 35 31 nucleotides only. The discovery makes them simplest known ribozymes. Moreover, circular permutated nearly identical catalytic internal loops, supported by stems...
Aim: To reconstruct the ancestral sequence of human adenoviral hexon protein by combining variations and structural information. And to provide a candidate for developing new vector capable escaping pre-existing immunity in healthy populations. Methods: The sequences 74 adenovirus-type strains were used predict adenovirus using FastML MEGA software. three-dimensional structure model was built homology modeling methods. immunological features loop 1 2 regions tested segments expressed...
<title>Abstract</title> Despite their importance in a wide range of living organisms, self-cleaving ribozymes human genome are few and poorly studied. Here, we performed deep mutational scanning covariance analysis two previously proposed (LINE-1 OR4K15 ribozymes). We found that the functional regions for both made short segments, connected by non-functional loop with total 46 47 contiguous nucleotides only. The discovery makes them shortest known ribozymes. Moreover, above LINE-1 circular...
<title>Abstract</title> Despite their importance in a wide range of living organisms, self-cleaving ribozymes human genome are few and poorly studied. Here, we performed deep mutational scanning covariance analysis two previously proposed (LINE-1 OR4K15). We found that the functional regions for both made short segments, connected by non-functional loop with total 46 47 contiguous nucleotides only. The discovery makes them shortest known ribozymes. Moreover, above circular permutated nearly...
Despite their importance in a wide range of living organisms, self-cleaving ribozymes human genome are few and poorly studied. Here, we performed deep mutational scanning covariance analysis two previously proposed (LINE-1 OR4K15 ribozymes). We found that the functional regions for both made short segments, connected by non-functional loop with total 46 47 contiguous nucleotides only. The discovery makes them shortest known ribozymes. Moreover, above LINE-1 circular permutated nearly...