A5023625643- Research in Cotton Cultivation
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- Nuclear Structure and Function
- Animal Disease Management and Epidemiology
- Leaf Properties and Growth Measurement
- Galectins and Cancer Biology
- Metal-Catalyzed Oxygenation Mechanisms
- Glycosylation and Glycoproteins Research
- Polysaccharides and Plant Cell Walls
- Advanced Cellulose Research Studies
- Carbohydrate Chemistry and Synthesis
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Stress Responses and Tolerance
- Molecular Biology Techniques and Applications
- Muscle Physiology and Disorders
- T-cell and Retrovirus Studies
- Plant biochemistry and biosynthesis
- Soil Management and Crop Yield
- Agricultural pest management studies
University of Technology Sydney
2018-2022
Commonwealth Scientific and Industrial Research Organisation
2016-2021
Agriculture and Food
2020-2021
Bioprocessing Technology Institute
2021
Huazhong Agricultural University
2008-2020
Jiangxi Academy of Agricultural Sciences
2020
Animal Science Research Institute
2020
ACT Government
2016
Hospital for Sick Children
1997-2007
SickKids Foundation
1997
Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple copies that belong to At and Dt subgenomes. Sequence similarity also very high between gene homologues. To efficiently achieve site/gene-specific mutation quite needed. Due its efficiency robustness, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system has exerted broad site-specific genome editing from prokaryotes eukaryotes. In this study, we utilized CRISPR/Cas9 generate...
As the most important natural raw material for textile industry, cotton fibres are an excellent model studying single-cell development. Although expression profiling and functional genomics have provided some data, mechanism of fibre development is still not well known. A class I TCP transcription factor (designated GbTCP), encoding 344 amino acids, was isolated from normalized cDNA library sea-island (from –2 to 25 days post anthesis). GbTCP preferentially expressed in elongating 5 15...
Abstract The cotton (Gossypium spp.) fiber is a unique elongated cell that useful for investigating differentiation. Previous studies have demonstrated the importance of factors such as sugar metabolism, cytoskeleton, and hormones, which are commonly known to be involved in plant development, while secondary metabolites been less regarded. By mining public data comparing analyses from two species hirsutum Gossypium barbadense), we found flavonoid metabolism active early development....
Summary Fiber elongation is the key determinant of fiber quality and output in cotton ( G ossypium hirsutum ). Although expression profiling functional genomics provide some data, mechanism development still not well understood. Here, a gene encoding calcium sensor, h C M 7 , was isolated based on its high level relative to other s cells at fast stage. The wild‐type fuzzless/lintless mutant correspond presence absence, respectively, initials. Overexpressing promotes early elongation, whereas...
Pectins are fundamental polysaccharides in the plant primary cell wall. synthesized and secreted to walls as highly methyl-esterified polymers then demethyl-esterified by pectin methylesterases (PMEs), which spatially regulated methylesterase inhibitors (PMEIs). Although PME PMEI genes pivotal wall formation, few studies have focused on evolutionary patterns of gene families. In this study, origin, evolution, expression diversity these two families were systematically analyzed using 11...
Abstract Cotton (Gossypium spp.) fiber cells are seed trichomes derived from the epidermal layer of cotton coat. The molecular components responsible for regulating cell differentiation have not been fully elucidated. A PROTODERMAL FACTOR1 gene (GbPDF1) was found to be expressed preferentially during initiation and early elongation, with highest accumulation in 5 d post anthesis. PDF1 silencing caused retardation produced shorter fibers lower lint percentage compared wild type, indicating...
Summary Cotton provides us the most important natural fibre. High fibre quality is major goal of cotton breeding, and introducing genes conferring longer, finer stronger from G ossypium barbadense to hirsutum an breeding strategy. We previously analysed G. development mechanism by gene expression profiling found two homoeologous fibre‐specific α‐expansins , Gb EXPA 2 EXPATR . (from D T genome) a classical α‐expansin, while its homoeolog, ( A genome), encodes truncated protein lacking normal...
Although biochemists and geneticists have studied the cotton flower for more than one century, little is known about molecular mechanisms underlying dramatic color change that occurs during its short developmental life following blooming. Through analysis of world germplasms, we found all flowers underwent changes post-anthesis, but there a diverse array petal colors among species, with cream, yellow red dominating scheme. Genetic biochemical analyses indicated both original cream...
Summary Cotton fibres, the single‐celled trichomes derived from ovule epidermis, provide most important natural material for global textile industry. A number of studies have demonstrated that regulating endogenous hormone levels through transgenic approaches can improve cotton fibre qualities. Phytosulfokine‐α ( PSK ‐α) is a novel peptide in plants involved cell proliferation and elongation. However, its potential applications crop genetic improvement not been evaluated. In this study, we...
Aims This study aimed to investigate the antimicrobial resistance (AMR) profiles and genotypes of Streptococcus suis from Jiangxi Province, China. Methods Results A total 314 nasal swab samples were collected clinically healthy pigs, with a positive isolation rate S. 34·08%. Antimicrobial susceptibility testing showed that more than 80% isolates susceptible vancomycin, penicillin, minocycline chloramphenicol. high frequency clindamycin, tetracycline, clarithromycin erythromycin was observed....
Cotton fiber is the most important natural fiber. Development of colorful cotton varieties with super quality has always been an goal genetics and breeding research. Flavonoids that are associated formation pigment plant secondary metabolites. In this review, we describe advances flavonoid pathway in fiber: brown pigments may mainly belong to proanthocyanidins. The down-regulated white domestication process, but cotton, gene expression profiling data show genes involved still very active...
The cotton ovules culture was innovated by Beasley and Ting (1973), named after them. It is a convenient system to analyze the effect of chemical or environmental treatment on fiber development directly ovules. This protocol generated according previous published papers our practical experience.