A5100398106- Animal Virus Infections Studies
- Research in Cotton Cultivation
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Immune Response and Inflammation
- Aquaculture disease management and microbiota
- Plant Molecular Biology Research
- Dyeing and Modifying Textile Fibers
- Microbial infections and disease research
- Viral Infections and Immunology Research
- Invertebrate Immune Response Mechanisms
- Animal Disease Management and Epidemiology
- Drug Transport and Resistance Mechanisms
- Advanced biosensing and bioanalysis techniques
- Antimicrobial Peptides and Activities
- Neuroscience of respiration and sleep
- Plant Gene Expression Analysis
- Vagus Nerve Stimulation Research
- Insect symbiosis and bacterial influences
- Immune Cell Function and Interaction
- Biosensors and Analytical Detection
- Herpesvirus Infections and Treatments
- RNA Interference and Gene Delivery
- Plant nutrient uptake and metabolism
- MicroRNA in disease regulation
Huzhou Central Hospital
2024
Zhejiang Chinese Medical University
2024
Huzhou University
2024
Zhejiang University
2024
Huazhong Agricultural University
2015-2022
Wuhan Polytechnic University
2022
South China Normal University
2019-2020
Center of Hubei Cooperative Innovation for Emissions Trading System
2017-2020
State Key Laboratory of Agricultural Microbiology
2015
China Agricultural University
2013
Graphene oxide and its derivatives have been widely explored for their antimicrobial properties due to high surface-to-volume ratios unique chemical physical properties. However, little information is available on effects viruses. In this study, we report the broad-spectrum antiviral activity of GO against pseudorabies virus (PRV, a DNA virus) porcine epidemic diarrhea (PEDV, an RNA virus). Our results showed that significantly suppressed infection PRV PEDV 2 log reduction in titers at...
The cotton fibre serves as a valuable experimental system to study cell wall synthesis in plants, but our understanding of the genetic regulation this process during development remains limited. We performed genome-wide association (GWAS) and identified 28 loci associated with quality allotetraploid cotton. To investigate regulatory roles these loci, we sequenced transcriptomes 251 accessions 15 330 expression quantitative trait (eQTL). Analysis local eQTL GWAS data prioritised 13 likely...
The new porcine epidemic diarrhea (PED) has caused devastating economic losses to the swine industry worldwide. Despite extensive research on relationship between autophagy and virus infection, concrete role of in (PEDV) infection not been reported. In this study, was demonstrated be triggered by effective replication PEDV through transmission electron microscopy, confocal Western blot analysis. Moreover, confirmed benefit using regulators RNA interference. Furthermore, might associated with...
Since 2010, the variant porcine epidemic diarrhea virus (PEDV) has been etiological agent responsible for outbreak of (PED) worldwide. In this study, a PEDV strain YN1 was isolated, serially propagated on Vero cells and characterized 200 passages. To better elucidate molecular basis cell adaptation strains, we sequenced, compared, analyzed full-genome sequences parental passages 15, 30, 60, 90, 144, 200. The results showed that variations increased with viral passage. nucleotides...
Epigenetic modifications are highlighted for their great importance in regulating plant development, but function associated with single-cell differentiation remains undetermined. Here, we used the cotton fibre, which is epidermal hair on ovule, as a model to investigate regulatory role of DNA methylation cell differentiation. The level CHH (H = A, T, or C) was found increase during fibre accompanied by decrease RNA-directed (RdDM). Examination nucleosome positioning revealed gradual...
Calcium (Ca2+) is necessary for fiber cell development in cotton (Gossypium hirsutum), both as a wall structural component and environmental signaling responses. It also known that potassium (K+) plays critical role elongation. However, it unclear whether Ca2+ integrates its activities with K+ to regulate Here, we report the novel discovery deficiency, when integrated signaling, promotes Using inductively coupled plasma-mass spectrometry (ICP-MS), determined dynamic profiles of ionome ovules...
For the last decade, porcine epidemic diarrhea virus (PEDV) variant strains have caused severe damage to global pig industry. Until now, no effective antivirals been developed for therapeutic treatment of PEDV infection. In present study, we found that quercetin significantly suppressed infection at noncytotoxic concentrations. A molecular docking study indicated might bind active site and binding pocket 3C-like protease (3CLpro). Surface plasmon resonance (SPR) analysis revealed exhibited a...
Abstract Red foliated cotton is a typical dominant mutation trait in upland (Gossypium hirsutum). Although mutants have been described, few responsible genes identified and characterized. In this study, we performed map-based cloning of the red mutant gene (Re) derived from cross between G. hirsutum cv. Emian22 barbadense acc. 3–79. Through expression profiling, metabolic pathway analysis, sequencing candidate genes, Re was as an MYB113 transcription factor. A repeat sequence variation...
Because the onset of fever induced by intravenously (i.v.) injected bacterial endotoxic lipopolysaccharides (LPS) precedes appearance in bloodstream pyrogenic cytokines, presumptive peripheral triggers febrile response, we have postulated previously that, their stead, PGE2 could be trigger because it appears blood coincidentally with initial body core temperature (Tc) rise. To test this hypothesis, Salmonella enteritidis LPS (2 microg/kg wt i.v.) into conscious guinea pigs and measured...
Porcine epidemic diarrhea virus (PEDV), a predominant cause of acute enteric infection, leads to severe dehydrating and mortality in piglets all over the world. A virulent PEDV YN13 strain, isolated our laboratory, was attenuated yield an strain YN144. To better understand pathogenesis mechanism virus-host interaction during infection with both YN144 strains, comparative proteomic analysis carried out investigate changes produced primary target organ, using isobaric tags for relative...
The sensitive and accurate detection methods for PEDV antibody have practical significance the prevention treatment of PEDV. In this work, a new multiple pathways signal amplification method was proposed to construct electrochemiluminescence (ECL) platform antibody. Using Au NP-modified graphene nanosheet (Au-GN) as substrate, antibody–antigen reaction recognition unit, rolling circle (RCA) enhancement, assembled cascade Ru-DNA nanotags label, behaved with good specificity sensitivity....
The nucleocapsid (N) protein is a major structural component of porcine epidemic diarrhea virus (PEDV), which predicted to be multifunctional in viral replication. Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) cellular participating the splicing pre-mRNA nucleus and translation regulation cytoplasm. According our previous proteomic study about PEDV infection vivo, hnRNP was thought factor influencing In this report, N discovered colocalize with perinuclear region infected cells....
To assess the relative contributions of different phagocytes to febrile response guinea pigs intravenous (i.v.) and intraperitoneal (i.p.) bacterial endotoxic lipopolysaccharide (LPS), we injected fluorescein isothiocyanate (FITC)-labeled LPS at doses 37.5, 75, 150, 300 900 microg/kg, measured its distribution corresponding core temperature (T(c)) changes before 15, 30, 60, 90, 120 min after injection. At all times, i.v. FITC-LPS appeared as granular fluorescent patches in circulating...