A5060442951- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Autophagy in Disease and Therapy
- Influenza Virus Research Studies
- interferon and immune responses
- Viral Infections and Immunology Research
- Respiratory viral infections research
- RNA modifications and cancer
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Herpesvirus Infections and Treatments
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Animal Disease Management and Epidemiology
- Vector-borne infectious diseases
- Parasitic Infections and Diagnostics
- Virology and Viral Diseases
- HIV Research and Treatment
- Mosquito-borne diseases and control
Washington University in St. Louis
2022-2024
Huazhong Agricultural University
2015-2023
Center of Hubei Cooperative Innovation for Emissions Trading System
2017-2023
State Key Laboratory of Agricultural Microbiology
2022
Influenza A virus (IAV) infection induces mitophagy, which is essential for the clearance of damaged mitochondria. Dysfunctional mitochondria can be selectively targeted by PINK1, recruits PRKN/PARK2 and leads to subsequent mitochondrial sequestration within autophagosomes. The IAV PB1-F2 protein translocates mitochondria, accelerates fragmentation impairs innate immunity. However, whether mediates IAV–induced mitophagy relation between PB1-F2-attenuated immunity remain obscure. Here, we...
Autophagy impacts the replication cycle of many viruses. However, role autophagy machinery in IAV remains unclear. Therefore, we explored detailed mechanisms utilized by to promote its replication. We demonstrated that NP- and M2-mediated promotes regulating AKT-mTOR signaling pathway HSP90AA1 expression. The interaction PB2 results increase viral RNA synthesis first; subsequently binding NP LC3 favors vRNP export, later M2 leads an production infectious particles, thus accelerating progeny...
Influenza A virus can evade host innate immune response that is involved in several viral proteins with complicated mechanisms. To date, how influenza M2 protein modulates the immunity remains unclear. Herein, we showed colocalized and interacted MAVS (mitochondrial antiviral signaling protein) on mitochondria, positively regulated MAVS-mediated immunity. Further studies revealed induced reactive oxygen species (ROS) production was required for activation of macroautophagy/autophagy...
Cryptosporidiosis is a major cause of severe diarrheal disease in infants from resource poor settings. The majority infections are caused by the human-specific pathogen C. hominis and absence vitro growth platforms has limited our understanding host-pathogen interactions development effective treatments. To address this problem, we developed stem cell-derived culture system for using human enterocytes differentiated under air-liquid interface (ALI) conditions. Human ALI cultures supported...
The new porcine epidemic diarrhea (PED) has caused devastating economic losses to the swine industry worldwide. Despite extensive research on relationship between autophagy and virus infection, concrete role of in (PEDV) infection not been reported. In this study, was demonstrated be triggered by effective replication PEDV through transmission electron microscopy, confocal Western blot analysis. Moreover, confirmed benefit using regulators RNA interference. Furthermore, might associated with...
Since 2010, the variant porcine epidemic diarrhea virus (PEDV) has been etiological agent responsible for outbreak of (PED) worldwide. In this study, a PEDV strain YN1 was isolated, serially propagated on Vero cells and characterized 200 passages. To better elucidate molecular basis cell adaptation strains, we sequenced, compared, analyzed full-genome sequences parental passages 15, 30, 60, 90, 144, 200. The results showed that variations increased with viral passage. nucleotides...
N6-methyladenosine (m6A) modification on viral RNAs has a profound impact infectivity. m6A is also highly pervasive for influenza RNAs. However, its role in virus mRNA splicing largely unknown. Here, we identify the reader protein YTHDC1 as host factor that associates with A NS1 and modulates splicing. levels are enhanced by IAV infection. We demonstrate inhibits NS binding to an 3' site promotes replication pathogenicity vitro vivo. Our results provide mechanistic understanding of IAV-host...
ABSTRACT A porcine deltacoronavirus (PDCoV) was identified in the Chinese mainland and found to be closely related Hong Kong strain HKU15-155 but differed from PDCoV strains United States South Korea. The complete genome of CH/SXD1/201 sequenced analyzed further characterize swine.
Several avian influenza A viruses (IAVs) have adapted to mammalian species, including humans. To date, the mechanisms enabling these host shifts remain incompletely understood. Here, we show that a factor, human TRA2A (huTRA2A), inhibits IAV replication, but benefits replication by altered regulation of viral messenger RNA (mRNA) splicing. huTRA2A depresses mRNA splicing binding intronic silencer motif in M representative YS/H5N1 or NS PR8/H1N1 virus, leading completely opposite effects on...
Rotaviruses (RVs) are one of the main causes severe gastroenteritis, diarrhea, and death in children young animals. While suckling mice prove to be highly useful small animal models RV infection pathogenesis, direct visualization tools lacking track temporal dynamics replication transmissibility vivo . Here, we report generation first recombinant murine-like that encodes a Nano-Luciferase reporter (NLuc) using newly optimized reverse genetics system. The NLuc-expressing was...
Rotaviruses pose a significant threat to young children. To identify novel pro- and anti-rotavirus host factors, we performed genome-wide CRISPR/Cas9 screens using rhesus rotavirus African green monkey cells. Genetic deletion of either SERPINB1 or TMEM236, the top two antiviral in MA104 cells increased virus titers strain independent manner. Using this information, optimized existing reverse genetics systems by combining knockout with C3P3-G3 helper plasmid. We improved recovery efficiency...
The complete genome sequence of a novel pseudorabies virus, strain HNB, isolated from dead weaned pig in China, was determined using next-generation sequencing. viral HNB shared 90.6% nucleotide similarity with that the traditional vaccine strain, Bartha strain.
Porcine rotavirus-A (PoRVA) is one of the common causes mild to severe dehydrating diarrhea, leading losses in weaning and postweaning piglets. A rapid, highly specific, sensitive antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) was developed for detection PoRVA, by using VP6 (a conserved antigenic protein group-A rotavirus)-directed rabbit polyclonal antibodies (capture antibody) murine monoclonal (detector antibody). The limit AC-ELISA found be equal that conventional reverse...
Influenza A viruses (IAVs) are a major global health threat and in the future, may cause next pandemic. Although studies have partly uncovered molecular mechanism of IAV-host interaction, it requires further research. In this study, we explored roles transportin-3 (TNPO3) IAV infection. We found that TNPO3-deficient cells inhibited infection with four different strains, whereas restoration TNPO3 expression knockout (KO) restored overexpression wild-type (WT) promoted infection, suggesting is...
Type I interferon (IFN-I), produced by the innate immune system, plays an essential role in host antiviral responses. Proper regulation of IFN-I production is required for to balance responses and prevent superfluous inflammation. IFN regulatory factor 3 (IRF3) subsequent sensors are activated RNA virus infection induce production. Therefore, proper IRF3 serves as important way control immunity viral replication. Here, we first identified Prohibitin1 (PHB1) a negative regulator...
AbstractRotaviruses are rising as zoonotic viruses worldwide, causing the lethal dehydrating diarrhea in children, piglets, and other livestock of economic importance. A simple, swift, cost-effective, highly specific, sensitive antigen-capture enzyme–linked immunosorbent assay (AC-ELISA) was developed for detection porcine rotavirus-A (PoRVA) by employing rabbit (capture antibody) murine polyclonal antibodies (detector produced against VP6 PoRVA (RVA/Pig-tc/CHN/TM-a/2009/G9P23). Reactivity...
ABSTRACT Rotaviruses (RVs) are one of the main causes severe gastroenteritis, diarrhea, and death in children young animals. Although suckling mice prove to be highly useful small animal models RV infection pathogenesis, direct visualization tools lacking track temporal dynamics replication transmissibility vivo . Here, we report generation first recombinant murine that encodes a Nano-Luciferase reporter (NLuc) using newly optimized reverse genetics system. The NLuc-expressing was...
N6-methyladenosine (m6A) modification on viral RNAs has profound impact infectivity. m6A is also a highly pervasive for influenza RNAs. However, its role in virus mRNA splicing largely unknown. Here, we identify the reader protein YTHDC1 as host factor that associates with A NS1 and modulates splicing. levels are enhanced by IAV infection. We demonstrate inhibits NS binding to 530A at 3′ site promotes replication pathogenicity vitro vivo. Our results provide mechanistic understanding of...