A5017103454- Animal Virus Infections Studies
- Fluorine in Organic Chemistry
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Sulfur-Based Synthesis Techniques
- Inorganic Fluorides and Related Compounds
- Catalytic C–H Functionalization Methods
- Second Language Learning and Teaching
- Biotin and Related Studies
- Recycling and Waste Management Techniques
- Herpesvirus Infections and Treatments
- Cyclopropane Reaction Mechanisms
- Peptidase Inhibition and Analysis
- Plant Virus Research Studies
- Vector-Borne Animal Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial infections and disease research
- Toxin Mechanisms and Immunotoxins
- Fecal contamination and water quality
- Groundwater flow and contamination studies
- Microplastics and Plastic Pollution
- Herbal Medicine Research Studies
Huazhong Agricultural University
2017-2025
Zhejiang University
2025
Center of Hubei Cooperative Innovation for Emissions Trading System
2017-2024
Ministry of Agriculture and Rural Affairs
2023-2024
Shanghai Zhangjiang Laboratory
2023-2024
Nanjing Normal University
2020-2024
Yantai University
2024
Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application
2020-2024
First Affiliated Hospital Zhejiang University
2024
Xi'an International Studies University
2021-2023
The new porcine epidemic diarrhea (PED) has caused devastating economic losses to the swine industry worldwide. Despite extensive research on relationship between autophagy and virus infection, concrete role of in (PEDV) infection not been reported. In this study, was demonstrated be triggered by effective replication PEDV through transmission electron microscopy, confocal Western blot analysis. Moreover, confirmed benefit using regulators RNA interference. Furthermore, might associated with...
Porcine epidemic diarrhea virus (PEDV) is a major pathogen that causes serious economic losses to the swine industry. To aid PEDV clinical diagnosis and vaccine development, sensitive precise serological methods are demanded for rapid detection of (neutralizing) antibodies. Aiming development novel virus-free hemagglutination inhibition (HI) assay, N-terminal region S1 subunit, encompassing sialic acid-binding motif, was first expressed as an Fc-fusion protein with C-terminal Spy Tag...
Microsecond pulsed electric field (µsPEF) is a newer treatment modality to replace catheter ablation of Atrial fibrillation (AF) due its fewer side effects. This study aims find out experimental parameters that effectively induce cardiomyocyte death and the precise mechanisms for microsecond fields (µsPEFs) cardiomyocytes. CCK8 flow apoptosis analysis were employed examine effects different µsPEFs on cardiomyocytes in vitro. The by which works explored through combination transcriptome...
Abstract An efficient protocol for the copper‐mediated trifluoromethylthiolation of heteroaryl bromides has been achieved using copper complex (bpy)Cu(SCF 3 ) as trifluoromethylthiolating reagent. This procedure provides a straightforward synthetic method trifluoromethyl sulfides from readily available, simple starting materials. The reaction demonstrates broad substrate scope and tolerates wide array functional groups, including nitrile, ester, chloro, nitro, or methoxy substituents. magnified image
Porcine deltacoronavirus (PDCoV) is an emerging swine coronavirus that causes severe diarrhea, resulting in high mortality neonatal piglets. Despite widespread outbreaks many countries, no effective PDCoV vaccines are currently available. Here, we generated, for the first time, a full-length infectious cDNA clone of PDCoV. We further manipulated by replacing NS6 gene with green fluorescent protein (GFP) to generate rPDCoV-ΔNS6-GFP; likewise, rPDCoV-ΔNS7 was constructed removing ATG start...
HEIs (Higher education institutions) in mainland China are making enormous efforts to implement internationalization. As a result, EMI (English-medium instruction) courses growing rapidly number and popularity while relevant research is still insufficient comparison European countries. Besides, although much existing has explored students’ beliefs attitudes towards EMI, little known on whether their may change over time or after the completion of course, experiences different differ. This...
The tandem synthesis of 2-trifluoromethylthio(seleno)-substituted benzofurans using palladium-catalyzed conditions is reported. Trifluoromethylthio(seleno)lation 2-(2,2-dibromovinyl)phenols with (bpy)CuSCF3 or [(bpy)CuSeCF3]2 furnishes several 2-trifluoromethylthio(seleno)lated in acceptable to good yield. Mechanistic investigations were performed elucidate the reaction pathway, which was involved formation a 2-bromobenzofuran species. scope this protocol further extended preparation...
Abstract Coronaviruses (CoVs) have continuously posed a threat to human and animal health. However, existing antiviral drugs are still insufficient in overcoming the challenges caused by multiple strains of CoVs. And methods for developing multi‐target limited terms exploring drug targets with similar functions or structures. In this study, four rounds structural design modification on salinomycin were performed novel compounds. It was based strategy topological structure binding properties...
English-Medium Instruction (EMI) practices in the People's Republic of China (PRC) are still emerging and need further empirical investigation, comparison to European countries. Existing research Asian context is mainly based on students' teachers' perceptions policy-related concerns. There a remarkable lack studies assessing language content learning outcomes through objective tests. This study specifically reports data two EMI programs, namely Film Production International Trade, taught by...
Abstract African Swine Fever virus (ASFV), the causative agent of swine fever, is a highly lethal hemorrhagic affecting domestic pigs and wild boars. The primary target cells for ASFV infection are porcine alveolar macrophages (PAMs), which difficult to obtain maintain in vitro, less subjective genetic editing. To overcome these issues facilitate research, we obtained subclonal cell line PK1-C5 by subcloning LLC-PK1 that support stable proliferation. This consequential exhibited high levels...
English-medium instruction (EMI) has been spreading rapidly as the result of China's movement to internationalize its HEIs (higher education institutions). However, there is a dearth research studies on students' motivation in EMI contexts, which should not only explore Foreign Language Learning (FLL) isolation but highlights integrating both content and language learning complex. This paper specifically reports development anxiety over one semester compares three disciplines: International...
Abstract The rapid expansion of large-scale pig farming has brought about a surge in viral diseases with high morbidity rates and diverse manifestations. This widespread occurrence multiple farms inflicted severe economic losses on the global swine industry. Consequently, there is an urgent need for eco-friendly efficient antiviral drugs that can effectively combat viruses prevent such as PEDV, PRRSV, PRV, other infections. To this end, we conducted study activity cytotoxicity eleven...
G. parasuis can cause serious Glasser’s disease, which is characterized by polyarthritis, meningitis, and fibrinous polyserositis in pigs. It high morbidity mortality swine herds major economic losses to the global pig industry.
Herein, a copper-catalyzed 2,2,2-trifluoroethylthiolation reaction of aryl bromides and iodides with elemental sulfur, 1,1,1-trifluoro-2-iodoethane is described. The showed excellent functional group tolerance allowed the synthesis various substituted 2,2,2-trifluoroethyl thioethers good to yields. This transformation constitutes one-pot 2,2,2-trifluoroethylthiolated compounds from inexpensive, readily available starting materials. Utility protocol was further demonstrated in late-stage...
The development of a safe and effective COVID-19 vaccine is paramount importance to terminate the current pandemic. An adjuvant crucial for improving efficacy subunit COVID19 vaccine. α-Galactosylceramide (αGC) classical iNKT cell agonist which causes rapid production Th1- Th2-associated cytokines; we, therefore, expect that or Th2-skewing analogues αGC can better enhance immunogenicity receptor-binding domain in spike protein SARS-CoV-2 fused with Fc region human IgG (RBD-Fc). Herein, we...