A5013212608- Influenza Virus Research Studies
- Knee injuries and reconstruction techniques
- Total Knee Arthroplasty Outcomes
- Immune Cell Function and Interaction
- Stellar, planetary, and galactic studies
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Animal Virus Infections Studies
- Osteoarthritis Treatment and Mechanisms
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- interferon and immune responses
- Respiratory viral infections research
- Sports injuries and prevention
- Viral Infections and Immunology Research
- Antimicrobial Peptides and Activities
- Mosquito-borne diseases and control
- Astronomy and Astrophysical Research
- Astronomical and nuclear sciences
- HIV Research and Treatment
- Viral gastroenteritis research and epidemiology
- Astrophysics and Star Formation Studies
Zhengzhou University
2025
Huazhong Agricultural University
2017-2024
Hunan University
2021-2024
Purple Mountain Observatory
2023-2024
University of Science and Technology of China
2023-2024
Qilu Hospital of Shandong University
2024
Zero to Three
2024
Center of Hubei Cooperative Innovation for Emissions Trading System
2018-2023
Kunming Medical University
2018-2022
First Affiliated Hospital of Kunming Medical University
2018-2022
Porcine deltacoronavirus (PDCoV) is an emerging swine coronavirus that causes severe diarrhea, resulting in high mortality neonatal piglets. Despite widespread outbreaks many countries, no effective PDCoV vaccines are currently available. Here, we generated, for the first time, a full-length infectious cDNA clone of PDCoV. We further manipulated by replacing NS6 gene with green fluorescent protein (GFP) to generate rPDCoV-ΔNS6-GFP; likewise, rPDCoV-ΔNS7 was constructed removing ATG start...
Porcine deltacoronavirus (PDCoV) was first detected in Hong Kong and has recently spread to many countries around the world. PDCoV causes acute diarrhea vomiting pigs, resulting significant economic losses global pork industry. In this study, a Chinese strain, designated CHN-HG-2017, isolated from feces of suckling piglet with severe watery on farm located central China. Subsequently, virus identified by an indirect immunofluorescence assay electron microscopy. A nucleotide sequence...
Neuraminidase is suggested as an important component for developing a universal influenza vaccine. Targeted induction of neuraminidase-specific broadly protective antibodies by vaccinations challenging. To overcome this, we rationally select the highly conserved peptides from consensus amino acid sequence globular head domains neuraminidase. Inspired B cell receptor evolution process, reliable sequential immunization regimen designed to result in immuno-focusing steering bulk immune...
It is beneficial to calibrate the period Wesenheit metallicity relation (PWZR) of Delta Cephei stars (DCEPs), i.e., classical Cepheids, using accurate parallaxes associated open clusters (OCs) from Gaia data release 3 (DR3). To this aim, we obtain a total 43 OC-DCEPs (including 33 fundamental mode, 9 first overtone and 1 multimode DCEPs.) PWZR as $W_G=(-3.356 \,\pm\, 0.033) \,(\log{P-1})+(-5.947 0.025)+(-0.285 0.064)[\textrm{Fe/H}]$. The concurrently obtained residual parallax offset in OC,...
Background: To explore the location accuracy and early clinical outcomes of using a 3D-printed individualized navigation template to assist in reconstruction anterior cruciate ligament (ACL). Methods: A single center randomized control study was conducted. Patients with ACL injury were treated conventional operation or an assisted by (the 3D group). The primary endpoint actual compared planned position. Results: There 20 23 participants group group, respectively. no differences bone tunnel...
Influenza virus infection poses a continual menace to public health. Here, we developed soluble trimeric HA ectodomain vaccines by establishing interprotomer disulfide bonds in the stem region, which effectively preserve native antigenicity of epitopes. The stable H1 proteins exhibited higher thermal stabilities comparison with unmodified HAs and showed strong binding activities towards panel anti-stem cross-reactive antibodies that recognize either or intraprotomer Negative stain...
Metal binding sites, antigen epitopes and drug sites are the hotspots in viral proteins that control how viruses interact with their hosts. virusMED ( virus M etal E pitopes D rug sites) is a rich internet application based on database of atomic interactions around 7041 experimentally determined protein structures. 25306 from 805 strains 75 families were characterized, including influenza, HIV-1 SARS-CoV-2 viruses. Just as Google Maps organizes annotates points interest, presents positions...
Abstract Background Animal model of Knee Osteoarthritis (OA) is the primary testing methodology for studies on pathogenic mechanisms and therapies human OA disease. Recent major modeling methods are divided into artificially induced spontaneous. However, these have some disadvantages slow progression, high cost no correlation with pathogenesis OA. Methods Our attempted to find a rapid, easy, consistent natural pathological process method by intra-articular injection stromal cell-derived...
Duck Tembusu virus (DTMUV), an emergent flavivirus, causes domestic waterfowls to suffer from severe egg-drop syndrome and fatal encephalitis, greatly threatens duck production globally. Like other mosquito-borne flaviviruses, the envelope (E) protein of all DTMUV strains was N-glycosylated at amino acid position 154. Thus far, biological roles E glycosylation have remained largely unexplored. Herein, we demonstrated key in replication pathogenicity ducks by characterizing...
Objective To investigate the short-term effectiveness of total knee arthroplasty (TKA) assisted by three-dimensional (3D) printing osteotomy navigation template. Methods A retrospective study was performed on 60 patients with osteoarthritis bewteen January 2016 and June 2017. Thirty cases underwent TKA 3D template (3D group) 30 conventional (conventional group). There no significant difference in gender, age, body mass index, surgical side, disease duration between 2 groups ( P>0.05). The...
Enhancing cross-protections against diverse influenza viruses is desired for vaccinations. Neuraminidase (NA)-specific antibody responses have been found to independently correlate with a broader protection spectrum. Here, we report sequential immunization regimen that includes priming NA protein followed by boosting peptide nanoclusters, which targeted enhancement of in BALB/c mice certain cross-protective B-cell epitopes was achieved. The nanoclusters were fabricated via desolvation...
Background The reaserch of artificial intelligence (AI) model for predicting spinal refracture is limited to bone mineral density, X‐ray and some conventional laboratory indicators, which has its own limitations. Besides, it lacks specific indicators related osteoporosis imaging factors that can better reflect quality, such as computed tomography (CT). Objective To construct a novel based on turn‐over markers CT identify patients who were more inclined suffer spine refracture. Methods images...
The frequent occurrence of mutations in the SARS-CoV-2 Spike (S) protein, with up to dozens mutations, poses a severe threat current efficacy authorized COVID-19 vaccines. Membrane (M) which is most abundant viral structural exhibits high level amino acid sequence conservation. M protein ectodomain could be recognized by specific antibodies; however, extent it immunogenic and provides protection remains unclear.
<title>Abstract</title> The conserved hemagglutinin (HA) stem region is widely regarded as a highly promising target for the development of universal influenza vaccine. Through structure-based design and iterative mutagenesis screening, we successfully engineered soluble trimeric H1 stem-only protein, designated #219, which does not contain heterologous trimerization motifs thus prevents off-target immune responses. As confirmed by 2.97 Å-resolution cryo-EM structure, overall conformation...
Scaffold‑based bone tissue engineering has therapeutic potential in the regeneration of osseous defects. The present study aimed to explore adhesion and cell viability a co‑culture system composed vascular endothelial cells PI<sup>‑</sup>/Annexin V<sup>+</sup> represents early apoptotic cells, PI<sup>+</sup>/Annexin V<sup>+</sup> late cells (VECs) adipose‑derived stem cells (ADSCs) on partially deproteinized biologic bone (PDPBB) <em>in vitro</em>, determine...
Abstract Using young open clusters and O–B2-type stars in Gaia Data Release 3, we investigate the kinematics of local spiral structure. In general, sources outer arms may present larger peculiar motions than those inner arms. The appear to have smaller stars, both Perseus Local Arms show an inward motion toward Galactic center rotate slower rotation. Meanwhile, Carina Arm move opposite direction from Sun marginally faster addition, using improved distance estimations kinematic methods for...
Influenza virus hemagglutinin (HA) stem is currently regarded as an extremely promising immunogen for designing universal influenza vaccines. The appropriate antigen-presenting vaccine vector would be conducive to increasing the immunogenicity of HA antigen. In this study, we generated chimeric virus-like particles (cVLPs) co-displaying truncated C-terminal DnaK from Escherichia coli and H1 or full-length antigen using baculovirus expression system. Transmission electronic micrography...
Aggressive rehabilitation after anterior cruciate ligament (ACL) reconstruction may result in better clinical outcomes and fewer complications such as knee stiffness weakness. We explored the effect of Chinese knotting technique (CKT) for aggressive ACL reconstruction. Ninety-one anatomical cases from 2016 to 2020 were retrospectively reviewed. All patients operated by same senior physician his team. According with or without CKT, divided into 2 groups. Both groups received rehabilitation....