Abstract Heterologous expression is the main approach for recombinant protein production ingenetic synthesis, which codon optimization necessary. The existing methods are based on biological indexes. In this paper, we propose a novel method deep learning. First, introduce concept of boxes, via DNA sequences can be recoded into box while ignoring order bases. Then, problem converted to sequence annotation corresponding amino acids with boxes. models Escherichia Coli were trained by...

Cystic echinococcosis (hydatid disease), caused by the tapeworm Echinococcus granulosus (class Cestoda; family Taeniidae), is a neglected tropical disease that results in morbidity and mortality millions of humans, as well huge economic losses livestock industry globally. Proteins from tetraspanin parasites have recently become regarded crucial molecules interaction with hosts parasitism are therefore suitable for development vaccines diagnostic agents. However, no information available to...

Neuraminidase is suggested as an important component for developing a universal influenza vaccine. Targeted induction of neuraminidase-specific broadly protective antibodies by vaccinations challenging. To overcome this, we rationally select the highly conserved peptides from consensus amino acid sequence globular head domains neuraminidase. Inspired B cell receptor evolution process, reliable sequential immunization regimen designed to result in immuno-focusing steering bulk immune...

Background Dermatomyositis (DM) is prone to nasopharyngeal carcinoma (NPC), but the mechanism unclear. This study aimed explore potential pathogenesis of DM and NPC. Methods The datasets GSE46239, GSE142807, GSE12452, GSE53819 were downloaded from GEO dataset. disease co-expression module was obtained by R-package WGCNA. We built PPI networks for key modules. ClueGO used analyze functional enrichment DEG analysis performed with "limma". “pROC” applied assess diagnostic performance hub genes....

Echinococcus granulosus is the causative agent of cystic echinococcosis with medical and veterinary importance in China. Our main objective was to discuss genotypes genetic diversity E. present domestic animals humans western A total 45 hydatid cyst samples were collected from sheep, humans, a yak subjected an analysis sequences mitochondrial cytochrome b (cytb) gene. The amplified PCR product for all 1,068 bp band. phylogenetic showed that identified as (genotype G1). Ten haplotypes...

Cystic echinococcosis, caused by Echinococcus granulosus , is a widespread parasitic zoonosis causing economic loss and public health problems. Annexins are important proteins usually present in the plasma membrane, but previous studies have shown that an annexin B33 protein of E. (Eg-ANX) could be detected excretory/secretory products cyst fluid. In this study, we cloned characterized Eg-ANX. silico analysis showed amino acid sequence Eg-ANX was conserved lacked any signal peptides. The...

As a cryptic species complex, the taxonomy of Echinococcus granulosus has long been controversial. The complete mitochondrial (mt) genome E. G3 genotype was sequenced and characterized. mt 13,607 bp in length, contained 2 ribosomal RNAs (rRNAs), 22 transfer (tRNAs), 12 protein-coding genes, with gene order identical to those other reported genotypes. overall nucleotide composition (coding strand) for sequence 19.1% A, 47.8% T, 25.0% G, 8.1% C, T being most common base C least common.

Abstract The field of deep learning has witnessed dramatic and rapid progress in the past several years, largely driven by availability massive datasets increased computational power. Although promising advances have been made, implementation drug design is still vigorously developing stage. Here we summarize frontiers emerging applications design. We provide background architecture important neural networks for discovery, including convolutional networks, recurrent transformer,...

Echinococcus granulosus is the etiological agent of cystic echinococcosis, a major zoonotic disease both humans and animals. In this study, we assessed genetic variability structure E. in Tibet plateau, using complete mitochondrial 16 S ribosomal RNA gene for first time. We collected sequenced 62 isolates from 3 populations plateau. A BLAST analysis indicated that 61 belonged to sensu stricto (genotypes G1–G3), while one isolate canadensis (genotype G6). detected haplotypes with haplotype...

We evaluated genetic diversity and structure of Echinococcus granulosus by analyzing the complete mitochondrial NADH dehydrogenase subunit 2 ( ND2 ) gene in 51 isolates E. sensu stricto metacestodes collected at three locations this region. detected 19 haplotypes, which formed a distinct clade with standard sheep strain (G1). Hence, all were identified as (G1–G3). Genetic relationships among haplotypes not associated geographical divisions, fixation indices (Fst) sampling localities low....

Echinococcus granulosus is a harmful cestode parasite that causes cystic echinococcosis in humans as well various livestock species and wild animals. Calmodulin (CaM), Ca2+ sensor protein, widely expressed eukaryotes mediates variety of cellular signaling activities.In the present study, cDNA encoding CaM (rEgCaM) was successfully cloned molecular biochemical characterizations carried out. The antigenicity immunoreactivity rEgCaM detected preliminary enzyme-linked immunosorbent assay...

Cystic echinococcosis (CE) is an important worldwide zoonotic disease that causes large economic losses and human suffering.Echinococcus granulosus, the causative agent of CE, exhibits different genotypes in locations.In order to identify its analyze genetic structure on Tibetan Plateau, we collected 72 hydatid cysts from intermediate hosts amplified sequenced their mitochondrial cytochrome c oxidase subunit 2 (cox2) genes.Seventy isolates were identified as E. granulosus G1 genotype, while...

Influenza virus infection poses a continual menace to public health. Here, we developed soluble trimeric HA ectodomain vaccines by establishing interprotomer disulfide bonds in the stem region, which effectively preserve native antigenicity of epitopes. The stable H1 proteins exhibited higher thermal stabilities comparison with unmodified HAs and showed strong binding activities towards panel anti-stem cross-reactive antibodies that recognize either or intraprotomer Negative stain...

Abstract Pathogen‐mediated balancing selection is commonly considered to play an important role in the maintenance of genetic diversity, particular immune genes. However, factors that may influence which genes are targets such largely unknown. To address this, here we focus on Pattern Recognition Receptor (PRR) signalling pathways, a key innate immunity. We used whole‐genome resequencing data from population bank voles ( Myodes glareolus ) test for associations between selection, pleiotropy...

Bamboo belongs to subfamily Bambusoideae of the grass family and is one most important non-timber forest resources in world. The AP2/ERF superfamily a group plant specific transcription factors plays roles regulation growth responses biotic abiotic stress. By comprehensively analyzing recently uncovered whole genome sequence full length cDNA moso bamboo (Phyllostachys edulis), 142 were identified 53 among them are supported by cDNA. Compared with recent report on identification 116 from P....

Abstract Differences in immune function between species could be a result of interspecific divergence coding sequence and/or expression genes. Here, we investigate how the degree and differs functional categories genes, if differences occur independently other factors (expression level, pleiotropy). To this end, compared spleen transcriptomes wild-caught yellow-necked mice bank voles. Immune genes expressed were divided into four depending on encoded protein: pattern recognition receptors...

Different host species often differ considerably in susceptibility to a given pathogen, but the causes of such differences are rarely known. The natural hosts tick-transmitted bacterium Borrelia afzelii, which is one causative agents Lyme borreliosis humans, include variety small mammals like voles and mice. Previous studies have shown that B. afzelii-infected bank (Myodes glareolus) about ten times higher bacterial load than infected yellow-necked mice (Apodemus flavicollis), indicating...

An interactive learning environment with logic-based, convenient operation and high efficiency for mobile is in great urgent need. In this paper, a knowledge network based on geometry problem solving processes generated automatically by rule-based automated reasoning engine. Then an model designed students these networks to simulate the real of students. Finally, some experiments are carried out demonstrate effectiveness environment.