Abstract Background Cachexia is a multifactorial metabolic syndrome with high morbidity and mortality in patients advanced cancer. The diagnosis of cancer cachexia depends on objective measures clinical symptoms history weight loss, which lag behind disease progression have limited utility for the early cachexia. In this study, we performed nuclear magnetic resonance‐based metabolomics analysis to reveal profile establish diagnostic model. Methods Eighty‐four patients, 33 pre‐cachectic 105...

As the most important natural raw material for textile industry, cotton fibres are an excellent model studying single-cell development. Although expression profiling and functional genomics have provided some data, mechanism of fibre development is still not well known. A class I TCP transcription factor (designated GbTCP), encoding 344 amino acids, was isolated from normalized cDNA library sea-island (from –2 to 25 days post anthesis). GbTCP preferentially expressed in elongating 5 15...

Summary Cotton provides us the most important natural fibre. High fibre quality is major goal of cotton breeding, and introducing genes conferring longer, finer stronger from G ossypium barbadense to hirsutum an breeding strategy. We previously analysed G. development mechanism by gene expression profiling found two homoeologous fibre‐specific α‐expansins , Gb EXPA 2 EXPATR . (from D T genome) a classical α‐expansin, while its homoeolog, ( A genome), encodes truncated protein lacking normal...

Plants of the genus Taxus have attracted much attention owing to natural product taxol, a successful anti-cancer drug. T. fuana and yunnanensis are two endangered species mainly distributed in Himalayas. In our study, an untargeted metabolomics approach integrated with targeted UPLC-MS/MS method was applied examine metabolic variations between these growing different environments. The level taxol is higher than that fuana, indicating economic value for production. A series specific...

This study aimed to optimize the dark tea pile-fermentation conditions through single factor and orthogonal experiments. The optimal consisted of 38 g/100g moisture content wet green tea, 45 °C temperature, 85 humidity. On this basis, dynamic changes flavor components during were systematically explored by chromatography, mass spectrometry other methods. During pile-fermentation, observed in internal ratio following various substances, with a decrease polyphenols (especially ester...

The effect of immunotherapy is limited by oncometabolite D-2-hydroxyglutarate (D2HG). D2HGDH an inducible enzyme that converts D2HG into the endogenous metabolite 2-oxoglutarate. We aimed to evaluate impairment CD8 T lymphocyte function in high-D2HG environment and explore phenotypic features anti-tumor D2HGDH-modified CAR-T cells. treatment inhibited expansion human lymphocytes cells, increased their glucose uptake, suppressed effector cytokine production, decreased central memory cell...

Flaxseed (Linum usitatissimum L.), one kind of common oilseeds, has many biologically active compounds including α-linolenic acid (ALA), lignan and dietary fiber. At present, the research flaxseed is focused on biological clinical studies its compounds. However, protein obtained generally from cake, are relatively limited. As other kinds plant proteins, presents excellent functionalities which can be used for food applications. Many evidence have showed that hydrolysates provide health...

Taxus species are well-known for paclitaxel, which exhibits antitumor activities and is used treating various cancers. Although most widespread in many areas, few studies have characterized the variation metabolism among different species. Using an integrated approach involving 'tandem mass tag' labeling liquid chromatography–tandem spectrometry (HPLC–MS), proteomes of T. media mairei were investigated 4078 proteins quantified. Screening classification differentially expressed revealed...

Cotton (Gossypium spp.) is an important economic crop and there obvious heterosis in cotton, fertility has played role this heterosis. However, the genes that exhibit critical roles anther development are not well understood. Here, we report acyl-CoA N-acyltransferase (EC2.3; GhACNAT) plays a key fertility. Suppression of GhACNAT by virus-induced gene silencing transgenic cotton (G. hirsutum L. cv. C312) resulted indehiscent anthers were full pollen, diminished filaments stamens, plant...

This study aimed to investigate the association between these Blood pressure (BP) components examined in this study, including systolic blood (SBP), diastolic (DBP), pulse (PP), and mean arterial (MAP), with presence of multiple carotid plaques a low-income rural Chinese population. population-based cross-sectional was conducted from April 2014 January 2015, involving participants Tianjin Brain Study. Participants aged 45 years older diagnosed were included. Data on demographics, medical...

Gibberellic acids (GAs) are plant hormones that play fundamental roles in growth and developmental processes. Previous studies have demonstrated three key enzymes of GA20ox, GA3ox, GA2ox involved GA biosynthesis. In this study, the Arabidopsis DREB1A gene driven by CaMV 35S promoter was introduced into soybean plants Agrobacterium- mediated transformation. The results showed transgenic exhibited a typical phenotype GA-deficient mutants, such as severe dwarfism, small dark-green leaves, late...

Purpose: To investigate the effect of astragaloside IV (AS-IV) on mitochondrial-dependent apoptosis in dorsal root ganglion diabetic peripheral neuropathy (DPN) rats through SIRT1/p53 pathway. Methods: Diabetic rat model was induced by high-carbohydrate/high-fat diet and intraperitoneal injection STZ. were divided into three groups (n =16 per group): DPN group, AS-IV group (60mg/kg/d) α-lipoic acid (ALA) (60mg/kg/d). Weight blood glucose levels monitored every 4 weeks for 12 weeks. evaluated...

As an important abiotic stress factor, ultraviolet-B (UV-B) light can stimulate the accumulation of antioxidants in plants. In this study, possibility enhancing antioxidant capacity pakchoi (Brassica rapa L.) by UV-B supplementation was assessed. Irradiation with 4 µmol·m-2·s-1 for h or 2 24 significantly increased 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and total reductive capacity, as a result inducing greater polyphenols flavonoids without affecting plant biomass. A high...

Accumulated oncometabolites in the tumor microenvironment (TME) suppresses metabolism, expansion, and function of T cells. Immunosuppressive TME also impeded Chimeric Antigen Receptor (CAR)-T cells mediated cytotoxicity since CAR-T had to adapt vivo metabolic characteristics with high levels oncometabolites. We screened for inhibition glucose uptake CD8 + found Kynurenine (Kyn) showed strongest inhibiting effect on uptake. In vitro experiments that 120 μM Kyn treatment resulted expansion...

Primary biliary cholangitis (PBC) is a chronic autoimmune liver disease associated with profound metabolic changes. The purpose of this study was to identify distinctive signature from the training set 29 PBC patients, 30 hepatitis B virus (HBV)-caused cirrhosis (HBC) and 41 healthy controls, validate applicability stability model validation 21 7 (AIH) 9 HBC. sera were investigated using high resolution nuclear magnetic resonance (NMR) datasets analyzed pairwise pattern recognition methods....

The flavonoids in the extracts of Ginkgo leaves have been shown to great medical value: thus, a method increase flavonoid contents these is significant importance for human health. In present study, we investigated changes and corresponding gene expression levels post-harvest after various treatments. We found that both ultraviolet-B NaCl treatment induced accumulation. However, analysis showed increases were achieved by different pathways. Furthermore, responded differently compared with...

TCP transcription factors play important roles in diverse aspects of plant development as transcriptional activators or repressors. However, the functional mechanisms TCPs are not well understood, especially cotton fibers. Here, we identified a total 37 non-redundant proteins from diploid ( Gossypium raimondii ), which showed great diversity expression profile. GrTCP11, an ortholog AtTCP11, was preferentially expressed anthers and during fiber initiation secondary cell wall synthesis stages....

The anti-tumor vindoline and catharanthine alkaloids are naturally existed in Catharanthus roseus (C. roseus), an ornamental plant many tropical countries. Plant-specific TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors play important roles various developmental processes. However, the of C. TCPs (CrTCPs) terpenoid indole alkaloid (TIA) biosynthesis largely unknown. Here, a total 15 CrTCP genes were identified newly updated genome grouped into three major classes (P-type, C-type...

ABSTRACT Tuberculosis (TB), caused by Mycobacterium tuberculosis , is a major public health issue, particularly in developing countries, and thus effective diagnostic methods for TB remain central theme basic clinical research. To evaluate five antigens (38-kDa protein [38kDa], Rv3621c, Rv3618, 38kDa-ESAT-6 [38E6], Ag85B-HBHA [AH]) serological tests patients, we recruited 288 patients 201 healthy controls. The median IgG reactivity to 38kDa, 38E6, AH was higher than that Rv3618 Rv3621c...