A5008038127- Pharmacogenetics and Drug Metabolism
- Phytochemistry and Bioactive Compounds
- Cancer Treatment and Pharmacology
- Pharmacological Effects of Natural Compounds
- Chemotherapy-induced cardiotoxicity and mitigation
- Drug Transport and Resistance Mechanisms
- Nutrition and Health in Aging
- Muscle Physiology and Disorders
- CAR-T cell therapy research
- Computational Drug Discovery Methods
- Adipose Tissue and Metabolism
- Immunotherapy and Immune Responses
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Traditional Chinese Medicine Analysis
- Microbial Natural Products and Biosynthesis
- Cancer Mechanisms and Therapy
- Medicinal Plant Pharmacodynamics Research
- Fibroblast Growth Factor Research
- Metal complexes synthesis and properties
- Cancer Immunotherapy and Biomarkers
- COVID-19 Clinical Research Studies
- Toxin Mechanisms and Immunotoxins
- Metabolomics and Mass Spectrometry Studies
- Chronic Myeloid Leukemia Treatments
Shanghai Jiao Tong University
2014-2024
Shanghai Sixth People's Hospital
2014-2024
Shanghai Ocean University
2024
Shanghai University of Medicine and Health Sciences
2019-2020
Daqing Oilfield General Hospital
2011
Seoul National University
2009
Abstract Background Cachexia is a multifactorial metabolic syndrome with high morbidity and mortality in patients advanced cancer. The diagnosis of cancer cachexia depends on objective measures clinical symptoms history weight loss, which lag behind disease progression have limited utility for the early cachexia. In this study, we performed nuclear magnetic resonance‐based metabolomics analysis to reveal profile establish diagnostic model. Methods Eighty‐four patients, 33 pre‐cachectic 105...
Cancer cachexia is a multifactorial syndrome affecting the skeletal muscle. Previous clinical trials showed that treatment with MEK inhibitor selumetinib resulted in muscle anabolism. However, it conflicting MAPK/ERK pathway controls mass of The current study investigated therapeutic effect and mechanisms amelioration cancer cachexia. classical model was established via transplantation CT26 colon adenocarcinoma cells into BALB/c mice. on body weight, tumor growth, muscle, food intake, serum...
Cardioprotection of dexrazoxane (DZR) against doxorubicin (DOX)-induced cardiotoxicity is contentious and the indicator controversial. A pairwise comparative metabolomics approach was used to delineate potential metabolic processes in present study. Ninety-six BALB/c mice were randomly divided into two supergroups: tumor control groups. Each supergroup control, DOX, DZR, DOX plus DZR treatment resulted a steady increase 5-hydroxylysine, 2-hydroxybutyrate, 2-oxoglutarate, 3-hydroxybutyrate,...
Huanglian ( Rhizoma Coptidis ) as a popular herb has been used for the treatment of various diseases such diarrhea, eye inflammation and women's abdominal ailments. Alkaloids are considered to be responsible its pharmacological effects. In this investigation, six alkaloids (coptisine, epiberberine, berberine, jateorrhizine, palmatine magnoflorine) were systematically evaluated their inhibition cytochrome P450 isoforms (CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6 CYP3A4) in human liver microsomes...
The effects of regular organic solvents on the metabolic activities various human cytochromes P450 (P450s) have been reported. However, very little is known about their influence mediated by P450s in rat liver microsomes (RLM). purpose this study was to investigate such as methanol, acetonitrile, dimethyl sulfoxide (DMSO), acetone, and ethanol CYP1A, CYP2C, CYP2D, CYP2E, CYP3A-mediated metabolism using RLM. results showed that most enzymes appeared be solvent-dependent, tested probes were...
The clinical use of doxorubicin (DOX) is limited by its dose-dependent cardiotoxicity. present study investigated the effects scutellarin against DOX-induced cardiotoxicity in rats using pharmacodynamic and pharmacokinetic approaches. DOX (20 mg/kg) was injected intraperitoneally (i.p.) as a single dose, (5 mg/kg/day) intravenously (i.v.) for 3 days. Rats treated with showed acute indicated elevated serum lactate dehydrogenase (LDH) activity (4057.8 ± 107.2 vs. 2032.7 70.95), tissue...
Inhaled budesonide and salbutamol represent the most important frequently used drugs in asthmatic children during acute exacerbation. However, there is still no consensus about their resulting metabolic derangements; thus, this study was conducted to determine distinct profiles of these two drugs. A total 69 with asthma exacerbation were included, serum urine investigated using high-resolution nuclear magnetic resonance (NMR). metabolomics analysis performed a principal component orthogonal...
Accumulated oncometabolites in the tumor microenvironment (TME) suppresses metabolism, expansion, and function of T cells. Immunosuppressive TME also impeded Chimeric Antigen Receptor (CAR)-T cells mediated cytotoxicity since CAR-T had to adapt vivo metabolic characteristics with high levels oncometabolites. We screened for inhibition glucose uptake CD8 + found Kynurenine (Kyn) showed strongest inhibiting effect on uptake. In vitro experiments that 120 μM Kyn treatment resulted expansion...
The anthracycline antibiotic doxorubicin (DOX) is an effective anticancer agent, but its clinical use limited by dose-dependent cardiotoxicity. Scutellarin (SCU), a natural polyphenolic flavonoid, used as cardioprotective agent for infarction and ischemia-reperfusion injury. This study investigated the beneficial effect of SCU on DOX-induced chronic Rats were injected intraperitoneally (i. p.) with DOX (2.5 mg/kg) twice week four weeks then allowed to rest two establish cardiotoxicity animal...
The BCR-Abl tyrosine kinase inhibitor (TKI), nilotinib, was developed to surmount resistance or intolerance imatinib in patients with Philadelphia-positive chronic myelogenous leukemia. Recent studies have shown that nilotinib induces potent sensitization anticancer agents by blocking the functions of ABCB1/P-glycoprotein (P-gp) multidrug (MDR). However, changes P-gp expression function affect cardiac disposition and prolong presence both doxorubicin (DOX) doxorubicinol (DOXol) tissue, thus,...
Tumor cells have higher rates of glucose uptake and aerobic glycolysis to meet energy demands for proliferation metastasis. The characteristics increased uptake, accompanied with glycolysis, has been exploited the diagnosis cancers. Although much progress made, mechanisms regulating tumor production are still not fully understood. Here, we demonstrate that Pim-2 is required in colorectal cells. Our results show highly expressed cells, may be induced by nutrient stimulation. Activation led...
The activation of hepatic stellate cells (HSCs) is the main cause liver fibrosis. beneficial effects fibroblast growth factor (FGF) 19 on fibrosis were recently reported. S. miltiorrhiza as well derived bioactive chemical components has shown prominent antifibrotic in but mechanism still not fully understood. We aimed to investigate compounds from which exerts HSCs via regulating FGF19. FGF19 level culture media was determined by enzyme-linked immunosorbent assay. Cell proliferation measured...
Altered branched chain amino acids (BCAAs), including leucine, isoleucine, and valine, are frequently observed in patients with advanced cancer. We evaluated the efficacy of chimeric antigen receptor (CAR) T cell-mediated cancer cell lysis potential immune microenvironment BCAA supplementation deletion. increased killing percentage, while accelerating catabolism decreasing transporter decreased efficacy. thus designed BCKDK engineering CAR cells for reprogramming metabolism tumor based on...
The therapeutic response rates of patients to donepezil vary from 20% 60%, one the reasons is their genetic differences in donepezil-metabolizing enzymes, which directly influence liver metabolism. However, mechanism metabolism and that its enantiomers unknown. This study evaluated CYP2D6 polymorphisms elucidate stereoselective confirm association between steady-state plasma concentrations pharmaco-effective S-donepezil responses Han Chinese with Alzheimer's disease. vitro demonstrated...
Inflammatory responses are key contributors to cancer cachexia and foster a complex cascade of biological outcomes. Baicalin is natural compound derived from Scutellaria baicalensis that possesses anti-inflammatory properties in many diseases; therefore, the aim this study was verify whether baicalin could ameliorate CT26 adenocarcinoma-induced model. Tumour-bearing control mice were injected with adenocarcinoma cells phosphate-buffered saline (PBS), respectively, administered...
A sensitive and high-throughput LC-MS/MS method was established validated for the simultaneous quantification of seven probe substrate-derived metabolites (cocktail assay) assessing in vitro inhibition cytochrome P450 (CYP) enzymes pooled human liver microsomes. The acetaminophen (CYP1A2), hydroxy-bupropion (CYP2B6), n-desethyl-amodiaquine (CYP2C8), 4'-hydroxy-diclofenac (CYP2C9), 4'-hydroxy-mephenytoin (CYP2C19), dextrorphan (CYP2D6) 1'-hydroxy-midazolam (CYP3A4/5), together with internal...
Cachexia is common in cancer patients, with profound metabolic abnormalities response to malignant growth of and progressive catabolism host.
Inhibition of cytochrome P450 (CYP) and P-glycoprotein (P-gp) are regarded as the most frequent clinically important pharmacokinetic causes among various possible factors for drug-drug interactions. Scutellarin is a flavonoid which widely used treatment cardiovascular diseases. In this study, in vitro inhibitory effects scutellarin on six major human CYPs (CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP3A4) rat CYP2C7, CYP2C11, CYP2C79, CYP2D4, CYP3A2) activities were examined by using liquid...