A5035787308- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- thermodynamics and calorimetric analyses
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Exercise and Physiological Responses
- DNA Repair Mechanisms
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Insect and Pesticide Research
- Viral gastroenteritis research and epidemiology
- Genomics and Chromatin Dynamics
- Sperm and Testicular Function
- Ubiquitin and proteasome pathways
- Spaceflight effects on biology
- Protein Structure and Dynamics
- Muscle metabolism and nutrition
- Liver Disease Diagnosis and Treatment
- Vitamin C and Antioxidants Research
- Nitric Oxide and Endothelin Effects
- PARP inhibition in cancer therapy
- Viral Infectious Diseases and Gene Expression in Insects
- Muscle Physiology and Disorders
- RNA and protein synthesis mechanisms
- Connexins and lens biology
Yamaguchi University
2015-2024
Takeda Science Foundation
2016
University of Helsinki
2005
Åbo Akademi University
2005
University of Turku
2005
Turku Centre for Biotechnology
2005
University of Virginia
2004
Morehouse School of Medicine
2004
Emory University
2002-2004
Duke Medical Center
2004
SUMO (small ubiquitin-like modifier) modification regulates many cellular processes, including transcription. Although sumoylation often occurs on specific lysines within the consensus tetrapeptide ΨKxE, other modifications, such as phosphorylation, may regulate of a substrate. We have discovered PDSM (phosphorylation-dependent motif), composed site and an adjacent proline-directed phosphorylation (ΨKxExxSP). The highly conserved motif phosphorylation-dependent multiple substrates,...
Vascular endothelial growth factor (VEGF) induces angiogenesis by stimulating cell proliferation and migration, primarily through the receptor tyrosine kinase VEGF receptor2 (Flk1/KDR). Reactive oxygen species (ROS) derived from NAD(P)H oxidase are critically important in many aspects of vascular regulation, both small GTPase Rac1 gp91 phox critical components complex. A role VEGF-induced angiogenesis, however, has not been defined. In present study, electron spin resonance spectroscopy is...
Endothelial cell (EC) proliferation and migration are important for reendothelialization angiogenesis. We have demonstrated that reactive oxygen species (ROS) derived from the small GTPase Rac1-dependent NAD(P)H oxidase involved in vascular endothelial growth factor (VEGF)-mediated responses mainly through VEGF type2 receptor (VEGFR2). Little is known about underlying molecular mechanisms. IQGAP1 a scaffolding protein controls cellular motility morphogenesis by interacting directly with...
Abstract Heat-shock response is an adaptive to proteotoxic stresses including heat shock, and regulated by heat-shock factor 1 (HSF1) in mammals. Proteotoxic challenge all subcellular compartments the mitochondria. Therefore, there must be close connections between mitochondrial signals activity of HSF1. Here, we show that shock triggers nuclear translocation SSBP1, which involved replication DNA, a manner dependent on permeability transition pore ANT–VDAC1 complex direct interaction with...
Inflammatory bowel disease (IBD) involves infiltration of leukocytes into intestinal tissue, resulting in damage induced by reactive oxygen species (ROS). Pro-inflammatory cytokines and cell adhesion molecules (CAMs) play important roles this leukocytes. The heat shock factor 1 (HSF1) proteins (HSPs) the development IBD are unclear. In study, we examined HSF1 HSPs an animal model IBD, dextran sulfate sodium (DSS)-induced colitis. colitis worsened or was ameliorated HSF1-null mice transgenic...
Heat shock transcription factor 1 (HSF1) not only regulates expression of heat genes in response to elevated temperature, but is also involved developmental processes by regulating such as cytokine genes. However, we did know how HSF1 non-heat Here, show that constitutive binding the interleukin (IL)-6 promoter necessary for its maximal induction lipopolysaccharide (LPS) stimulation mouse embryo fibroblasts and peritoneal macrophages. Lack inhibited LPS-induced vivo an activator NF-kappaB a...
Abstract The febrile response is a complex physiological reaction to disease, including cytokine-mediated increase in body temperature and the activation of inflammatory systems. Fever has beneficial roles terms disease prognosis, partly by suppressing expression cytokines. However, molecular mechanisms underlining fever-mediated suppression gene have not been clarified. In this study, we showed that heat shock suppresses LPS-induced IL-6, major pyrogenic cytokine, mouse embryonic...
The heat-shock response is characterized by the expression of a set classical genes, and regulated transcription factor 1 (HSF1) in mammals. However, comprehensive analyses gene have revealed very large numbers inducible genes cells exposed to heat shock. It believed that HSF1 required for heat-inducible these although HSF2 HSF4 modulate some expression. Here, we identified novel mouse HSF3 (mHSF3) translocated into nucleus during mHSF3 did not activate such as Hsp70. Remarkably,...
Aberrant transcriptional regulation in the brain is thought to be one of key components pathogenesis and pathophysiology neuropsychiatric disorders. Heat shock factors (HSFs) modulate cellular homeostasis through control gene expression. However, roles HSFs function have yet elucidated fully. In present study, we attempted clarify role HSF1-mediated neuronal behavioral development using HSF1-deficient (HSF1 −/− ) mice. We found granule neurons aberrant morphology impaired neurogenesis...
Poly(ADP-ribose) polymerase 1 (PARP1) is involved in DNA repair, chromatin structure, and transcription. However, the mechanisms that regulate PARP1 distribution on are poorly understood. Here, we show heat shock transcription factor (HSF1) recruits through scaffold protein PARP13. In response to damage, activated auto-poly-ADP-ribosylated dissociates from HSF1-PARP13, redistributes lesions damage-inducible gene loci. Histone deacetylase maintains ternary complex by inactivating...
The mitochondrial unfolded protein response (UPR mt ) is characterized by the transcriptional induction of chaperone and protease genes in to impaired proteostasis regulated ATF5 CHOP mammalian cells. However, detailed mechanisms underlying UPR are currently unclear. Here, we show that HSF1 required for activation genes, including HSP60, HSP10, mtHSP70, mouse embryonic fibroblasts during inhibition matrix TRAP1, Lon, or electron transfer complex 1 activity. bound constitutively gene...
Quality-control mechanisms in spermatogenesis are important to eliminate injured or abnormal cells, thereby protecting the organism from development next generation. The processes of highly sensitive high temperatures; however, by which germ cells eliminated remain unclear. Here, we found that heat shock proteins not induced male response thermal stress, although transcription factor 1 (HSF1) is activated. Using HSF1-null mice, showed apoptosis pachytene spermatocytes was markedly inhibited...
Heat shock factor 1 (HSF1) is a major transactivator of heat proteins in response to shock, and it also involved oogenesis, spermatogenesis, placental development. However, we do not know the molecular mechanisms controlling developmental processes. In this study, found that HSF1-null mice exhibited significant decrease T cell-dependent B cell response. When were immunized intraperitoneally with sheep red blood cells, cell-specific IgG production, especially IgG2a was about 50% lower than...
The extracellular superoxide dismutase (ecSOD) plays an important role in atherosclerosis and endothelial function by modulating levels of the anion (O2*-) space. Although heparan sulfate proteoglycan is ligand for ecSOD, little known about other biological binding partners ecSOD. goal this study was to identify novel proteins that interact with A yeast two-hybrid screening a human aorta cDNA library using ecSOD as bait identified fibulin-5 predominant protein Further analysis showed domain...
Heat shock response is characterized by the induction of heat proteins (HSPs), which facilitate protein folding, and non-HSP with diverse functions, including degradation, regulated factors (HSFs). HSF1 a master regulator HSP expression during in mammals, as HSF3 avians. HSF2 plays roles development brain reproductive organs. However, fundamental vertebrate cells have not been identified. Here we find that activated physiological range. deficiency reduces threshold for chicken or mouse...
The heat shock response is an evolutionally conserved adaptive to high temperatures that controls proteostasis capacity and regulated mainly by ancient factor (HSF). However, the regulation of target genes stress-inducible HSF1 transcription complex has not yet been examined in detail mammalian cells. In present study, we demonstrated interacted with members ATF1/CREB family involved metabolic homeostasis recruited them on HSP70 promoter shock. complex, including chromatin-remodeling BRG1...
Heat shock response, which is characterized by the induction of a set heat proteins, essential for induced thermotolerance and regulated transcription factors (HSFs). Curiously, HSF1 response in mammals, whereas avian HSF3, an avian-specific factor required burst activation genes. Amino acid sequences chicken are highly conserved with human HSF1, but those HSF3 diverge significantly. Here, we demonstrated that lost ability to activate genes through amino-terminal domain containing...